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Literature summary extracted from

  • Jensen, K.F.; Mygind, B.
    Different oligomeric states are involved in the allosteric behavior of uracil phosphoribosyltransferase from Escherichia coli (1996), Eur. J. Biochem., 240, 637-645.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.2.9 GTP kinetics Escherichia coli
2.4.2.9 GTP highly activating at low 5-phosphoribose 1-diphosphate concentration, e.g. 0.2 mM, by modification of the structure from dimer/trimer to pentamer/hexamer Escherichia coli
2.4.2.9 GTP about 5-7fold increase of Km for 5-phosphoribose 1-diphosphate, unaltered Vmax Escherichia coli
2.4.2.9 guanosine-3',5'-diphosphate activates in the same range as GTP Escherichia coli

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.2.9 overexpressing from plasmid in Escherichia coli strain NF1815 Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.2.9 5-phosphoribose 1-diphosphate inhibitory when higher concentrated as Mg2+ Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.9 additional information
-
 additional information kinetics Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.2.9 Co2+ poor activator Escherichia coli
2.4.2.9 Mg2+ required Escherichia coli
2.4.2.9 Mn2+ can partly substitute for Mg2+ at 5 mM, less effective, inhibitory at higher concentration Escherichia coli
2.4.2.9 Zn2+ can partly substitute for Mg2+ at 5 mM, less effective, inhibitory at higher concentration Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.2.9 additional information
-
 gel filtration and sedimentation analyses: a smaller oligomeric, less active form, dimeric or trimeric, predominates in absence of substrates, a larger aggregated, more active form, pentameric or hexameric, predominates in presence of substrates Escherichia coli
2.4.2.9 22500
-
 x * 22500, SDS-PAGE Escherichia coli
2.4.2.9 63000
-
 sucrose density gradient centrifugation and gel filtration in absence of substrates Escherichia coli
2.4.2.9 111000
-
 sucrose density gradient centrifugation and gel filtration in presence of substrates Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.9 Escherichia coli
-
 gene upp
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.9 recombinant from overexpressing strain Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.2.9 7.5
-
 purified recombinant enzyme Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.9 5-fluorouracil + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Escherichia coli 5-fluoro-UMP + diphosphate
-
 ?
2.4.2.9 uracil + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Escherichia coli UMP + diphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.2.9 ? x * 22500, SDS-PAGE Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.2.9 37
-
 assay at Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.2.9 8.5
-
 assay at Escherichia coli