Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Alloush, H.M.; Kerridge, D.
    Characterization of a partially purified uracil phosphoribosyltransferase from the opportunistic pathogen Candida albicans (1994), Mycopathologia, 125, 129-141.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.4.2.9 enzyme is extremly unstable, UMP stabilizes during storage Candida albicans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.9 0.0705
-
 Uracil
-
 Candida albicans
2.4.2.9 0.186
-
 5-phospho-alpha-D-ribose 1-diphosphate
-
 Candida albicans

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.2.9 Mg2+ required Candida albicans
2.4.2.9 Mg2+ optimal concentration : 2 mM Candida albicans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.2.9 38000
-
 x * 47000 + x * 38000, SDS-PAGE Candida albicans
2.4.2.9 47000
-
 x * 47000 + x * 38000, SDS-PAGE Candida albicans

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.9 Candida albicans
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.9 only partial, due to the labile nature of the enzyme further purification is unsuccessful Candida albicans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.2.9 0.068
-
 partially purified enzyme Candida albicans

Storage Stability

EC Number Storage Stability Organism
2.4.2.9 4°C, Tris-HCl, pH 8.0, 1 mM EDTA, 2 mM DTT, 1 mM UMP, loss of 40% activity in 1 week Candida albicans
2.4.2.9 4°C, Tris-HCl, pH 8.0, 1 mM EDTA, 2 mM DTT, loss of 70% activity in 4 days Candida albicans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.9 5-fluorouracil + 5-phospho-alpha-D-ribose 1-diphosphate 1.6fold higher specific activity compared to uracil Candida albicans 5-fluoro-UMP + diphosphate
-
 ?
2.4.2.9 uracil + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Candida albicans UMP + diphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.2.9 ? x * 47000 + x * 38000, SDS-PAGE Candida albicans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.2.9 55
-
 4 min, complete loss of activity Candida albicans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.2.9 7.5
-
-
 Candida albicans