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Literature summary extracted from

  • Tsuchida, K.; Lind, T.; Kitagawa, H.; Lindahl, U.; Sugahara, K.; Lidholt, K.
    Purification and characterization of fetal bovine serum beta-N-acetyl-D-galactosaminyltransferase and beta-D-glucuronyltransferase involved in chondroitin sulfate biosynthesis (1999), Eur. J. Biochem., 264, 461-467.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.4.1.175 stable against freeze-thawing in liquid N2 several times Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.175 160000
-
 gel filtration Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.175 UDP-N-acetyl-alpha-D-galactosamine + chondroitin oligosaccharide Bos taurus involved in chondroitin biosynthesis UDP + N-acetyl-beta-D-galactosaminyl chondroitin oligosaccharide
-
 ?
2.4.1.175 UDP-N-acetyl-alpha-D-galactosamine + chondroitin sulfate oligosaccharide Bos taurus involved in chondroitin sulfate biosynthesis: elongation of growing chondroitin sulfate chain UDP + N-acetyl-beta-D-galactosaminyl chondroitin sulfate oligosaccharide
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.175 Bos taurus
-
 fetal
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.175
-
 Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.175 serum
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.175 0.0425
-
 purified enzyme Bos taurus

Storage Stability

EC Number Storage Stability Organism
2.4.1.175 -80°C, MES/NaOH, 50 mM, pH 6.5, 1 mM dithiothreitol, stable for at least a few months Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.175 additional information the enzyme also has the 3-beta-glucuronosyltransferase, EC 2.4.1.226, activity Bos taurus ?
-
 ?
2.4.1.175 UDP-N-acetyl-alpha-D-galactosamine + (glucuronyl-N-acetyl-1,3-beta-D-galactosamine)3 minimal chain length required for activity Bos taurus UDP + N-acetyl-beta-D-galactosaminyl-1,4-glucuronyl-N-acetyl-1,3-beta-D-galactosaminyl-(glucuronyl-N-acetyl-1,3-beta-D-galactosamine)2
-
 ?
2.4.1.175 UDP-N-acetyl-alpha-D-galactosamine + chondroitin oligosaccharide involved in chondroitin biosynthesis Bos taurus UDP + N-acetyl-beta-D-galactosaminyl chondroitin oligosaccharide
-
 ?
2.4.1.175 UDP-N-acetyl-alpha-D-galactosamine + chondroitin sulfate oligosaccharide involved in chondroitin sulfate biosynthesis: elongation of growing chondroitin sulfate chain Bos taurus UDP + N-acetyl-beta-D-galactosaminyl chondroitin sulfate oligosaccharide
-
 ?
2.4.1.175 UDP-N-acetyl-D-galactosamine + chondroitin oligosaccharide acceptors are even-numbered chondroitin oligosaccharides with terminal glucuronyl-N-acetyl-1,3-beta-D-galactosamine sequence at non-reducing end, transfers N-acetyl-galactosamine to non-reducing glucuronic acid residues in beta-glycosidic linkage Bos taurus UDP + N-acetyl-D-galactosaminyl chondroitin oligosaccharide
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.175 More the human form of this enzyme is a bifunctional glycosyltransferase, which also has the 3-beta-glucuronosyltransferase, EC 2.4.1.226, activity required for the synthesis of the chondroitin sulfate disaccharide repeats. Similar chondroitin synthase co-polymerases can be found in Pasteurella multocida and Escherichia coli. Bos taurus
2.4.1.175 More the enzyme also has the 3-beta-glucuronosyltransferase, EC 2.4.1.226, activity Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.175 6.5
-
 assay at Bos taurus