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Literature summary extracted from

  • Ninomiya, T.; Sugiura, N.; Tawada, A.; Sugimoto, K.; Watanabe, H.; Kimata, K.
    Molecular cloning and characterization of chondroitin polymerase from Escherichia coli strain K4 (2002), J. Biol. Chem., 277, 21567-21575.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.175 DNA and amino acid sequence determination and analysis, expression as soluble His-tagged protein in Escherichia coli strain TOP10 Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.175 Ca2+ complete inhibition Escherichia coli
2.4.1.175 Cu2+ complete inhibition Escherichia coli
2.4.1.175 EDTA complete inhibition Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.175 0.0316
-
 UDP-N-acetyl-beta-D-galactosamine
-
 Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.175 Fe2+ can partly substitute for Mn2+, 30.6% activity compared to Mn2+ Escherichia coli
2.4.1.175 Mg2+ can partly substitute for Mn2+, 30.7% activity compared to Mn2+ Escherichia coli
2.4.1.175 Mn2+ required, 20 mM Escherichia coli
2.4.1.175 Mn2+ can partly be substituted by Fe2+ and Mg2+ Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.175 UDP-N-acetyl-alpha-D-galactosamine + chondroitin oligosaccharide Escherichia coli biosynthesis of K4 antigen, a capsule polysaccharide consisting of a chondroitin backbone to which beta-fructose is linked at position C-3 of the GlcNAc residue UDP + N-acetyl-beta-D-galactosaminyl chondroitin oligosaccharide
-
 ?
2.4.1.175 UDP-N-acetyl-alpha-D-galactosamine + chondroitin sulfate oligosaccharide Escherichia coli
-
 UDP + N-acetyl-beta-D-galactosaminyl chondroitin sulfate oligosaccharide
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.175 Escherichia coli Q8L0V4
-
-
2.4.1.175 Escherichia coli K4 Q8L0V4
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.175 recombinant His-tagged enzyme expressed in E. coli strain TOP10 Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.175 0.00053
-
 purified rcombinant enzyme, substrate chondroitin polysaccharide with MW 10 kDa Escherichia coli
2.4.1.175 0.00124
-
 purified rcombinant enzyme, substrate chondroitin hexasaccharide Escherichia coli
2.4.1.175 0.00331
-
 purified rcombinant enzyme, substrate chondroitin sulfate polysaccharide with MW 20 kDa Escherichia coli
2.4.1.175 0.00334
-
 purified rcombinant enzyme, substrate chondroitin sulfate hexasaccharide Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.175 additional information the enzyme also has the 3-beta-glucuronosyltransferase, EC 2.4.1.226, activity Escherichia coli ?
-
 ?
2.4.1.175 additional information the enzyme also has the 3-beta-glucuronosyltransferase, EC 2.4.1.226, activity Escherichia coli K4 ?
-
 ?
2.4.1.175 UDP-N-acetyl-alpha-D-galactosamine + (glucuronyl-N-acetyl-1,3-beta-D-galactosamine)2 minimal required acceptor substrate chain length Escherichia coli UDP + N-acetyl-beta-D-galactosaminyl-1,4-glucuronyl-N-acetyl-1,3-beta-D-galactosaminyl-glucuronyl-N-acetyl-1,3-beta-D-galactosamine
-
 ?
2.4.1.175 UDP-N-acetyl-alpha-D-galactosamine + (glucuronyl-N-acetyl-1,3-beta-D-galactosamine)3
-
 Escherichia coli UDP + N-acetyl-beta-D-galactosaminyl-1,4-glucuronyl-N-acetyl-1,3-beta-D-galactosaminyl-(glucuronyl-N-acetyl-1,3-beta-D-galactosamine)2
-
 ?
2.4.1.175 UDP-N-acetyl-alpha-D-galactosamine + (glucuronyl-N-acetyl-1,3-beta-D-galactosamine)3
-
 Escherichia coli K4 UDP + N-acetyl-beta-D-galactosaminyl-1,4-glucuronyl-N-acetyl-1,3-beta-D-galactosaminyl-(glucuronyl-N-acetyl-1,3-beta-D-galactosamine)2
-
 ?
2.4.1.175 UDP-N-acetyl-alpha-D-galactosamine + chondroitin oligosaccharide biosynthesis of K4 antigen, a capsule polysaccharide consisting of a chondroitin backbone to which beta-fructose is linked at position C-3 of the GlcNAc residue Escherichia coli UDP + N-acetyl-beta-D-galactosaminyl chondroitin oligosaccharide
-
 ?
2.4.1.175 UDP-N-acetyl-alpha-D-galactosamine + chondroitin sulfate oligosaccharide
-
 Escherichia coli UDP + N-acetyl-beta-D-galactosaminyl chondroitin sulfate oligosaccharide
-
 ?
2.4.1.175 UDP-N-acetyl-beta-D-galactosamine + chondroitin sulfate hexasaccharide
-
 Escherichia coli ?
-
 ?
2.4.1.175 UDP-N-acetyl-D-galactosamine + chondroitin oligosaccharide acceptors are even-numbered chondroitin oligosaccharides with terminal glucuronyl-N-acetyl-1,3-beta-D-galactosamine sequence at non-reducing end, transfers N-acetyl-galactosamine to non-reducing glucuronic acid residues in beta-glycosidic linkage Escherichia coli UDP + N-acetyl-D-galactosaminyl chondroitin oligosaccharide transfer in beta-1,4-linkage ?
2.4.1.175 UDP-N-acetyl-D-galactosamine + chondroitin oligosaccharide acceptor substrates longer than a tetrasaccharide are absolutely required Escherichia coli UDP + N-acetyl-D-galactosaminyl chondroitin oligosaccharide transfer in beta-1,4-linkage ?
2.4.1.175 UDP-N-acetyl-D-galactosamine + chondroitin oligosaccharide acceptors are even-numbered chondroitin oligosaccharides with terminal glucuronyl-N-acetyl-1,3-beta-D-galactosamine sequence at non-reducing end, transfers N-acetyl-galactosamine to non-reducing glucuronic acid residues in beta-glycosidic linkage Escherichia coli K4 UDP + N-acetyl-D-galactosaminyl chondroitin oligosaccharide transfer in beta-1,4-linkage ?
2.4.1.175 UDP-N-acetyl-D-galactosamine + chondroitin oligosaccharide acceptor substrates longer than a tetrasaccharide are absolutely required Escherichia coli K4 UDP + N-acetyl-D-galactosaminyl chondroitin oligosaccharide transfer in beta-1,4-linkage ?
2.4.1.175 UDP-N-acetyl-D-galactosamine + chondroitin sulfate oligosaccharide acceptor substrates longer than a tetrasaccharide are absolutely required Escherichia coli UDP + N-acetyl-D-galactosaminyl chondroitin sulfate oligosaccharide
-
 ?
2.4.1.175 UDP-N-acetyl-D-galactosamine + chondroitin sulfate oligosaccharide acceptor substrates longer than a tetrasaccharide are absolutely required Escherichia coli K4 UDP + N-acetyl-D-galactosaminyl chondroitin sulfate oligosaccharide
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.175 chondroitin polymerase
-
 Escherichia coli
2.4.1.175 More the human form of this enzyme is a bifunctional glycosyltransferase, which also has the 3-beta-glucuronosyltransferase, EC 2.4.1.226, activity required for the synthesis of the chondroitin sulfate disaccharide repeats. Similar chondroitin synthase co-polymerases can be found in Pasteurella multocida and Escherichia coli. Escherichia coli
2.4.1.175 More the enzyme also has the 3-beta-glucuronosyltransferase, EC 2.4.1.226, activity Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.175 25
-
 highest product chain length Escherichia coli
2.4.1.175 30
-
 highest reaction rate Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.175 7 7.5
-
 Escherichia coli