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Literature summary extracted from

  • Uyama, T.; Kitagawa, H.; Tanaka, J.; Tamura, J.i.; Ogawa, T.; Sugahara, K.
    Molecular cloning and expression of a second chondroitin N-acetylgalactosaminyltransferase involved in the initiation and elongation of chondroitin/dermatan sulfate (2003), J. Biol. Chem., 278, 3072-3078.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.174 DNA sequence determination, genomic organization and chromosome localization, functional expression as soluble protein fused to protein A IgG-binding domain in COS-1 cells, secretion into the medium Homo sapiens
2.4.1.175 DNA sequence determination, genomic organization and chromosome localization, functional expression as soluble protein fused to protein A IgG-binding domain in COS-1 cells, secretion into the medium Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.174 membrane type II membrane protein topology Homo sapiens 16020
-
2.4.1.175 membrane type II membrane protein topology Homo sapiens 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.174 Mn2+
-
 Homo sapiens
2.4.1.175 Mn2+
-
 Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.174 95000
-
 x * 95000, recombinant enzyme, SDS-PAGE Homo sapiens
2.4.1.175 95000
-
 x * 95000, recombinant enzyme, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.175 UDP-N-acetyl-alpha-D-galactosamine + chondroitin oligosaccharide Homo sapiens involved in chondroitin biosynthesis UDP + N-acetyl-beta-D-galactosaminyl chondroitin oligosaccharide
-
 ?
2.4.1.175 UDP-N-acetyl-alpha-D-galactosamine + chondroitin sulfate oligosaccharide Homo sapiens involved in chondroitin sulfate biosynthesis: elongation of growing chondroitin sulfate chain UDP + N-acetyl-beta-D-galactosaminyl chondroitin sulfate oligosaccharide
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.174 Homo sapiens Q8N6G5
-
-
2.4.1.174 Homo sapiens Q8TDX6
-
-
2.4.1.175 Homo sapiens Q8N6G5 chondroitin N-acetylgalactosaminyltransferase II
-
2.4.1.175 Homo sapiens Q8TDX6 chondroitin N-acetylgalactosaminyltransferase I, not N-acetylgalactosaminyltransferase I EC 2.4.1.174
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.4.1.174 glycoprotein 2 potential N-glycosylation sites, either one or both of which are used in COS-1 cells during expression of the recombinant fusion protein Homo sapiens
2.4.1.175 glycoprotein 2 potential N-glycosylation sites, either one or both of which are used in COS-1 cells during expression of the recombinant fusion protein Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.174 leukocyte peripheral blood Homo sapiens
-
2.4.1.174 lung
-
 Homo sapiens
-
2.4.1.174 peripheral blood
-
 Homo sapiens
-
2.4.1.174 skeletal muscle
-
 Homo sapiens
-
2.4.1.175 leukocyte
-
 Homo sapiens
-
2.4.1.175 lung
-
 Homo sapiens
-
2.4.1.175 peripheral blood
-
 Homo sapiens
-
2.4.1.175 skeletal muscle
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.174 UDP-N-acetyl-D-galactosamine + beta-D-glucuronyl-1,3-beta-D-galactosyl-1,3-beta-D-galactosyl-1,4-beta-D-xylosyl-1-O-(Gly)Ser-(Gly-Glu)
-
 Homo sapiens UDP + N-acetyl-D-galactosamine-1,4-beta-D-glucuronyl-1,3-beta-D-galactosyl-1,3-beta-D-galactosyl-1,4-beta-D-xylosyl-1-O-(Gly)Ser-(Gly-Glu)
-
 ?
2.4.1.174 UDP-N-acetyl-D-galactosamine + beta-D-glucuronyl-1,3-beta-D-galactosyl-1,3-beta-D-galactosyl-1,4-beta-D-xylosyl-1-O-Ser
-
 Homo sapiens UDP + N-acetyl-D-galactosamine-1,4-beta-D-glucuronyl-1,3-beta-D-galactosyl-1,3-beta-D-galactosyl-1,4-beta-D-xylosyl-1-O-Ser
-
 ?
2.4.1.174 UDP-N-acetyl-D-galactosamine + beta-D-glucuronyl-1,3-beta-D-galactosyl-1,3-beta-D-galactosyl-1,4-beta-D-xylosyl-1-O-Ser-peptide
-
 Homo sapiens UDP + N-acetyl-D-galactosamine-1,4-beta-D-glucuronyl-1,3-beta-D-galactosyl-1,3-beta-D-galactosyl-1,4-beta-D-xylosyl-1-O-Ser-peptide
-
 ?
2.4.1.174 UDP-N-acetyl-D-galactosamine + beta-D-glucuronyl-1,3-beta-D-galactosyl-1-O-C2H4-NH-benzyloxycarbonyl
-
 Homo sapiens UDP + N-acetyl-D-galactosamine-1,4-beta-D-glucuronyl-1,3-beta-D-galactosyl-1-O-C2H4-NH-benzyloxycarbonyl
-
 ?
2.4.1.174 UDP-N-acetyl-D-galactosamine + glucuronylgalactosyl glycosides
-
 Homo sapiens UDP + N-acetyl-D-galactosaminyl-1,4-beta-D-glucuronylgalactosyl glycosides
-
 ?
2.4.1.175 additional information the enzyme shows no 3-beta-glucuronosyltransferase activity, EC 2.4.1.226 Homo sapiens ?
-
 ?
2.4.1.175 additional information the enzyme also has N-acetylgalactosaminyltransferase I activity, EC 2.4.1.174 Homo sapiens ?
-
 ?
2.4.1.175 UDP-N-acetyl-alpha-D-galactosamine + chondroitin oligosaccharide involved in chondroitin biosynthesis Homo sapiens UDP + N-acetyl-beta-D-galactosaminyl chondroitin oligosaccharide
-
 ?
2.4.1.175 UDP-N-acetyl-alpha-D-galactosamine + chondroitin sulfate oligosaccharide involved in chondroitin sulfate biosynthesis: elongation of growing chondroitin sulfate chain Homo sapiens UDP + N-acetyl-beta-D-galactosaminyl chondroitin sulfate oligosaccharide
-
 ?
2.4.1.175 UDP-N-acetyl-D-galactosamine + chondroitin oligosaccharide
-
 Homo sapiens UDP + N-acetyl-D-galactosaminyl chondroitin oligosaccharide
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.174 ? x * 95000, recombinant enzyme, SDS-PAGE Homo sapiens
2.4.1.175 ? x * 95000, recombinant enzyme, SDS-PAGE Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
2.4.1.174 chondroitin galactosaminyltransferase
-
 Homo sapiens
2.4.1.174 More
-
 Homo sapiens
2.4.1.174 More the enzyme has also N-acetylgalactosaminyltransferase II activity, EC 2.4.1.175, substrates sulfated and unsulfated chondroitin oligo- and polysaccharides Homo sapiens
2.4.1.175 chondroitin galactosaminyltransferase
-
 Homo sapiens
2.4.1.175 More the enzyme also has N-acetylgalactosaminyltransferase I activity, EC 2.4.1.174 Homo sapiens
2.4.1.175 More the human form of this enzyme is a bifunctional glycosyltransferase, which also has the 3-beta-glucuronosyltransferase, EC 2.4.1.226, activity required for the synthesis of the chondroitin sulfate disaccharide repeats. Similar chondroitin synthase co-polymerases can be found in Pasteurella multocida and Escherichia coli. Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.174 37
-
 assay at Homo sapiens
2.4.1.175 37
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.175 6.5
-
 assay at Homo sapiens