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Literature summary extracted from

  • Gotoh, M.; Sato, T.; Akashima, T.; Iwasaki, H.; Kameyama, A.; Mochizuki, H.; Yada, T.; Inaba, N.; Zhang, Y.; Kikuchi, N.; Kwon, Y.D.; Togayachi, A.; Kudo, T.; Nishihara, S.; Watanabe, H.; Kimata, K.; Narimatsu, H.
    Enzymatic synthesis of chondroitin with a novel chondroitin sulfate N-acetylgalactosaminyltransferase that transfers N-acetylgalactosamine to glucuronic acid in initiation and elongation of chondroitin sulfate synthesis (2002), J. Biol. Chem., 277, 38189-38196.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.174 DNA sequence determination and analysis, expression of a truncated form, consisting of the putative catalytic domain, in Spodoptera frugiperda Sf21 insect cells as a soluble FLAG-tagged protein Homo sapiens
2.4.1.175 DNA sequence determination and analysis, expression of a truncated form, consisting of the putative catalytic domain, in Spodoptera frugiperda Sf21 insect cells as a soluble FLAG-tagged protein Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.174 UDP-N-acetyl-D-galactosamine + glucuronylgalactosyl glycosides Homo sapiens involved in chondroitin initiation and elongation UDP + N-acetyl-D-galactosaminylglucuronylgalactosyl glycosides
-
 ?
2.4.1.174 UDP-N-acetyl-D-galactosamine + glucuronylgalactosyl glycosides Homo sapiens involved in the biosynthesis of chodroitin sulfate. Key enzyme activity for the initiation of chondroitin and dermatan sulfates, transferring GalNAc to the GlcA-Gal-Gal-Xyl-Ser core. UDP + N-acetyl-D-galactosaminylglucuronylgalactosyl glycosides
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.174 Homo sapiens Q8TDX6 chondroitin sulfate N-acetylgalactosaminyltransferase
-
2.4.1.175 Homo sapiens Q8TDX6 chondroitin sulfate N-acetylgalactosaminyltransferase
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.174 adrenal gland low content Homo sapiens
-
2.4.1.174 bone marrow
-
 Homo sapiens
-
2.4.1.174 brain
-
 Homo sapiens
-
2.4.1.174 cerebral cortex
-
 Homo sapiens
-
2.4.1.174 colon low content Homo sapiens
-
2.4.1.174 G-361 cell melanoma cell line Homo sapiens
-
2.4.1.174 kidney very low content Homo sapiens
-
2.4.1.174 leukocyte low content Homo sapiens
-
2.4.1.174 liver very low content Homo sapiens
-
2.4.1.174 lung low content Homo sapiens
-
2.4.1.174 ovary
-
 Homo sapiens
-
2.4.1.174 pancreas
-
 Homo sapiens
-
2.4.1.174 placenta very high content Homo sapiens
-
2.4.1.174 small intestine high content Homo sapiens
-
2.4.1.174 spleen
-
 Homo sapiens
-
2.4.1.174 SW-1736 cell thyroid cancer cell line Homo sapiens
-
2.4.1.174 testis low content Homo sapiens
-
2.4.1.174 thymus low content Homo sapiens
-
2.4.1.174 thyroid gland very high content Homo sapiens
-
2.4.1.174 U266 cell myeloma cell line Homo sapiens
-
2.4.1.174 uterus
-
 Homo sapiens
-
2.4.1.175 adrenal gland low content Homo sapiens
-
2.4.1.175 bone marrow
-
 Homo sapiens
-
2.4.1.175 brain
-
 Homo sapiens
-
2.4.1.175 cerebral cortex
-
 Homo sapiens
-
2.4.1.175 colon low content Homo sapiens
-
2.4.1.175 G-361 cell melanoma cell line Homo sapiens
-
2.4.1.175 kidney very low content Homo sapiens
-
2.4.1.175 leukocyte low content Homo sapiens
-
2.4.1.175 liver very low content Homo sapiens
-
2.4.1.175 lung low content Homo sapiens
-
2.4.1.175 ovary
-
 Homo sapiens
-
2.4.1.175 pancreas
-
 Homo sapiens
-
2.4.1.175 placenta very high content Homo sapiens
-
2.4.1.175 small intestine high content Homo sapiens
-
2.4.1.175 spleen
-
 Homo sapiens
-
2.4.1.175 SW-1736 cell thyroid cancer cell line Homo sapiens
-
2.4.1.175 testis low content Homo sapiens
-
2.4.1.175 thymus low content Homo sapiens
-
2.4.1.175 thyroid gland very high content Homo sapiens
-
2.4.1.175 U266 cell myeloma cell line Homo sapiens
-
2.4.1.175 uterus
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.174 additional information
-
-
 Homo sapiens
2.4.1.175 additional information
-
-
 Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.174 UDP-N-acetyl-D-galactosamine + beta-D-glucuronyl-1,3-beta-D-galactosyl-1,3-beta-D-galactosyl-beta-D-xylose-O-methoxyphenyl i.e. linkage tetrasaccharide, synthetic substrate Homo sapiens UDP + N-acetyl-D-galactosamine-1,4-beta-D-glucuronyl-1,3-beta-D-galactosyl-1,3-beta-D-galactosyl-beta-D-xylose-O-methoxyphenyl
-
 ?
2.4.1.174 UDP-N-acetyl-D-galactosamine + beta-D-glucuronyl-1,3-beta-D-galactosyl-1,3-beta-D-galactosyl-beta-D-xylose-O-methoxyphenyl best acceptor substrate together with chondroitin polysaccharide Homo sapiens UDP + N-acetyl-D-galactosamine-1,4-beta-D-glucuronyl-1,3-beta-D-galactosyl-1,3-beta-D-galactosyl-beta-D-xylose-O-methoxyphenyl
-
 ?
2.4.1.174 UDP-N-acetyl-D-galactosamine + beta-D-glucuronyl-1,3-beta-D-galactosyl-1,3-beta-D-galactosyl-beta-D-xylose-O-methoxyphenyl transfers GalNAc to GlcA at the non-reducing terminus of the linkage tetrasacharide Homo sapiens UDP + N-acetyl-D-galactosamine-1,4-beta-D-glucuronyl-1,3-beta-D-galactosyl-1,3-beta-D-galactosyl-beta-D-xylose-O-methoxyphenyl
-
 ?
2.4.1.174 UDP-N-acetyl-D-galactosamine + glucuronylgalactosyl glycosides
-
 Homo sapiens UDP + N-acetyl-D-galactosaminyl-1,4-beta-D-glucuronylgalactosyl glycosides
-
 ?
2.4.1.174 UDP-N-acetyl-D-galactosamine + glucuronylgalactosyl glycosides involved in chondroitin initiation and elongation Homo sapiens UDP + N-acetyl-D-galactosaminylglucuronylgalactosyl glycosides
-
 ?
2.4.1.174 UDP-N-acetyl-D-galactosamine + glucuronylgalactosyl glycosides involved in the biosynthesis of chodroitin sulfate. Key enzyme activity for the initiation of chondroitin and dermatan sulfates, transferring GalNAc to the GlcA-Gal-Gal-Xyl-Ser core. Homo sapiens UDP + N-acetyl-D-galactosaminylglucuronylgalactosyl glycosides
-
 ?
2.4.1.175 additional information low activity with sulfated oligosaccharide substrates Homo sapiens ?
-
 ?
2.4.1.175 additional information chondroitin sulfates A, C, D, and E, and dermatan sulfate are poor acceptor substrates Homo sapiens ?
-
 ?
2.4.1.175 UDP-N-acetyl-alpha-D-galactosamine + (glucuronyl-N-acetyl-1,3-beta-D-galactosamine)3
-
 Homo sapiens UDP + N-acetyl-beta-D-galactosaminyl-1,4-glucuronyl-N-acetyl-1,3-beta-D-galactosaminyl-(glucuronyl-N-acetyl-1,3-beta-D-galactosamine)2
-
 ?
2.4.1.175 UDP-N-acetyl-alpha-D-galactosamine + (glucuronyl-N-acetyl-1,3-beta-D-galactosamine)4
-
 Homo sapiens UDP + N-acetyl-beta-D-galactosaminyl-1,4-glucuronyl-N-acetyl-1,3-beta-D-galactosaminyl-(glucuronyl-N-acetyl-1,3-beta-D-galactosamine)3
-
 ?
2.4.1.175 UDP-N-acetyl-alpha-D-galactosamine + (glucuronyl-N-acetyl-1,3-beta-D-galactosamine)5
-
 Homo sapiens UDP + N-acetyl-beta-D-galactosaminyl-1,4-glucuronyl-N-acetyl-1,3-beta-D-galactosaminyl-(glucuronyl-N-acetyl-1,3-beta-D-galactosamine)4
-
 ?
2.4.1.175 UDP-N-acetyl-alpha-D-galactosamine + (glucuronyl-N-acetyl-1,3-beta-D-galactosamine)6
-
 Homo sapiens UDP + N-acetyl-beta-D-galactosaminyl-1,4-glucuronyl-N-acetyl-1,3-beta-D-galactosaminyl-(glucuronyl-N-acetyl-1,3-beta-D-galactosamine)5
-
 ?
2.4.1.175 UDP-N-acetyl-alpha-D-galactosamine + (glucuronyl-N-acetyl-1,3-beta-D-galactosamine)7
-
 Homo sapiens UDP + N-acetyl-beta-D-galactosaminyl-1,4-glucuronyl-N-acetyl-1,3-beta-D-galactosaminyl-(glucuronyl-N-acetyl-1,3-beta-D-galactosamine)6
-
 ?
2.4.1.175 UDP-N-acetyl-D-galactosamine + chondroitin oligosaccharide low activity Homo sapiens UDP + N-acetyl-D-galactosaminyl chondroitin oligosaccharide
-
 ?
2.4.1.175 UDP-N-acetyl-D-galactosamine + chondroitin oligosaccharide acceptors are even-numbered chondroitin oligosaccharides with terminal glucuronyl-N-acetyl-1,3-beta-D-galactosamine sequence at non-reducing end, transfers N-acetyl-galactosamine to non-reducing glucuronic acid residues in beta-glycosidic linkage Homo sapiens UDP + N-acetyl-D-galactosaminyl chondroitin oligosaccharide
-
 ?
2.4.1.175 UDP-N-acetyl-D-galactosamine + chondroitin polysaccharide best acceptor substrate Homo sapiens UDP + N-acetyl-D-galactosaminyl chondroitin polysaccharide
-
 ?
2.4.1.175 UDP-N-acetyl-D-galactosamine + chondroitin sulfate oligosaccharide
-
 Homo sapiens UDP + N-acetyl-D-galactosaminyl chondroitin sulfate oligosaccharide
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.174 More the enzyme has also N-acetylgalactosaminyltransferase II activity, EC 2.4.1.175, substrates chondroitin oligo- and polysaccharides Homo sapiens
2.4.1.175 More the enzyme also has N-acetylgalactosaminyltransferase I activity, EC 2.4.1.174 Homo sapiens
2.4.1.175 More the human form of this enzyme is a bifunctional glycosyltransferase, which also has the 3-beta-glucuronosyltransferase, EC 2.4.1.226, activity required for the synthesis of the chondroitin sulfate disaccharide repeats. Similar chondroitin synthase co-polymerases can be found in Pasteurella multocida and Escherichia coli. Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.174 37
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.174 6.5
-
 assay at Homo sapiens