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Literature summary extracted from

  • Hendershot, L.L.; Aeed, P.A.; Kezdy, F.J.; Elhammer, A.P.
    An efficient assay for dolichyl phosphate-mannose: protein O-mannosyltransferase (2002), Anal. Biochem., 307, 273-279.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.109 CuSO4 10 mM, complete inhibition Saccharomyces cerevisiae
2.4.1.109 glycerol at 50% inhibitory Saccharomyces cerevisiae
2.4.1.109 additional information no inhibition by EDTA Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.109 1
-
 Tyr-Asn-Pro-Thr-Ser-Val
-
 Saccharomyces cerevisiae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.109 membrane
-
 Saccharomyces cerevisiae 16020
-
2.4.1.109 spheroplast
-
 Saccharomyces cerevisiae
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.109 Mg2+ required, 10 mM Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.109 Saccharomyces cerevisiae
-
-
-
2.4.1.109 Saccharomyces cerevisiae BY4743
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.109 additional information
-
 assay development on microtiter plates Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.109 dolichyl phosphate D-mannose + Tyr-Asn-Pro-Thr-Ser-Val
-
 Saccharomyces cerevisiae dolichyl phosphate + O-D-mannosyl-Tyr-Asn-Pro-Thr-Ser-Val
-
 ?
2.4.1.109 dolichyl phosphate D-mannose + Tyr-Asn-Pro-Thr-Ser-Val
-
 Saccharomyces cerevisiae BY4743 dolichyl phosphate + O-D-mannosyl-Tyr-Asn-Pro-Thr-Ser-Val
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.109 PMT
-
 Saccharomyces cerevisiae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.109 20
-
 assay at Saccharomyces cerevisiae

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.4.1.109 additional information
-
 40% remaining activity at 0°C Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.109 7.5
-
 assay at Saccharomyces cerevisiae