BRENDA - Enzyme Database

An efficient assay for dolichyl phosphate-mannose: protein O-mannosyltransferase

Hendershot, L.L.; Aeed, P.A.; Kezdy, F.J.; Elhammer, A.P.; Anal. Biochem. 307, 273-279 (2002)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.4.1.109
CuSO4
10 mM, complete inhibition
Saccharomyces cerevisiae
2.4.1.109
glycerol
at 50% inhibitory
Saccharomyces cerevisiae
2.4.1.109
additional information
no inhibition by EDTA
Saccharomyces cerevisiae
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.4.1.109
1
-
Tyr-Asn-Pro-Thr-Ser-Val
-
Saccharomyces cerevisiae
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.4.1.109
membrane
-
Saccharomyces cerevisiae
16020
-
2.4.1.109
spheroplast
-
Saccharomyces cerevisiae
-
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.4.1.109
Mg2+
required, 10 mM
Saccharomyces cerevisiae
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.4.1.109
Saccharomyces cerevisiae
-
-
-
2.4.1.109
Saccharomyces cerevisiae BY4743
-
-
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
2.4.1.109
additional information
-
assay development on microtiter plates
Saccharomyces cerevisiae
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.1.109
dolichyl phosphate D-mannose + Tyr-Asn-Pro-Thr-Ser-Val
-
638343
Saccharomyces cerevisiae
dolichyl phosphate + O-D-mannosyl-Tyr-Asn-Pro-Thr-Ser-Val
-
638343
Saccharomyces cerevisiae
?
2.4.1.109
dolichyl phosphate D-mannose + Tyr-Asn-Pro-Thr-Ser-Val
-
638343
Saccharomyces cerevisiae BY4743
dolichyl phosphate + O-D-mannosyl-Tyr-Asn-Pro-Thr-Ser-Val
-
638343
Saccharomyces cerevisiae BY4743
?
Temperature Optimum [C]
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
2.4.1.109
20
-
assay at
Saccharomyces cerevisiae
Temperature Range [C]
EC Number
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
2.4.1.109
additional information
-
40% remaining activity at 0C
Saccharomyces cerevisiae
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.4.1.109
7.5
-
assay at
Saccharomyces cerevisiae
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.4.1.109
CuSO4
10 mM, complete inhibition
Saccharomyces cerevisiae
2.4.1.109
glycerol
at 50% inhibitory
Saccharomyces cerevisiae
2.4.1.109
additional information
no inhibition by EDTA
Saccharomyces cerevisiae
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.4.1.109
1
-
Tyr-Asn-Pro-Thr-Ser-Val
-
Saccharomyces cerevisiae
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.4.1.109
membrane
-
Saccharomyces cerevisiae
16020
-
2.4.1.109
spheroplast
-
Saccharomyces cerevisiae
-
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.4.1.109
Mg2+
required, 10 mM
Saccharomyces cerevisiae
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
2.4.1.109
additional information
-
assay development on microtiter plates
Saccharomyces cerevisiae
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.1.109
dolichyl phosphate D-mannose + Tyr-Asn-Pro-Thr-Ser-Val
-
638343
Saccharomyces cerevisiae
dolichyl phosphate + O-D-mannosyl-Tyr-Asn-Pro-Thr-Ser-Val
-
638343
Saccharomyces cerevisiae
?
2.4.1.109
dolichyl phosphate D-mannose + Tyr-Asn-Pro-Thr-Ser-Val
-
638343
Saccharomyces cerevisiae BY4743
dolichyl phosphate + O-D-mannosyl-Tyr-Asn-Pro-Thr-Ser-Val
-
638343
Saccharomyces cerevisiae BY4743
?
Temperature Optimum [C] (protein specific)
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
2.4.1.109
20
-
assay at
Saccharomyces cerevisiae
Temperature Range [C] (protein specific)
EC Number
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
2.4.1.109
additional information
-
40% remaining activity at 0C
Saccharomyces cerevisiae
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.4.1.109
7.5
-
assay at
Saccharomyces cerevisiae