BRENDA - Enzyme Database

Protein C-mannosylation is enzyme-catalysed and uses dolichyl-phosphate-mannose as a precursor

Doucey, M.A.; Hess, D.; Cacan, R.; Hofsteenge, J.; Mol. Biol. Cell 9, 291-300 (1998)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.4.1.109
chymotrypsin
inactivation
Rattus norvegicus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.4.1.109
membrane
-
Rattus norvegicus
16020
-
2.4.1.109
microsome
-
Rattus norvegicus
-
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.4.1.109
Mg2+
-
Rattus norvegicus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.4.1.109
dolichyl phosphate D-mannose + ribonuclease 2
Rattus norvegicus
C-mannosylation activity
dolichyl phosphate + ribonuclease 2-D-mannose
-
-
-
2.4.1.109
dolichyl phosphate D-mannose + ribonuclease 2
Rattus norvegicus
biosynthetic pathway
dolichyl phosphate + ribonuclease 2-D-mannose
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.4.1.109
Rattus norvegicus
-
C-mannosylating enzyme
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.4.1.109
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
2.4.1.109
additional information
-
-
Rattus norvegicus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.1.109
dolichyl phosphate D-mannose + human ribonuclease 2
whole protein or N-terminal dodecapeptide containing Trp7
638341
Rattus norvegicus
dolichyl phosphate + human ribonuclease 2-D-mannose
D-mannose is bound at Trp7 forming a C-C linkage
638341
Rattus norvegicus
?
2.4.1.109
dolichyl phosphate D-mannose + human ribonuclease 2
C-mannosylation activity
638341
Rattus norvegicus
dolichyl phosphate + human ribonuclease 2-D-mannose
D-mannose is bound at Trp7 forming a C-C linkage
638341
Rattus norvegicus
?
2.4.1.109
dolichyl phosphate D-mannose + human ribonuclease 2
no C-mannosylation activity of Trp7 when Trp10 is exchanged for Ala in the acceptor substrate
638341
Rattus norvegicus
dolichyl phosphate + human ribonuclease 2-D-mannose
D-mannose is bound at Trp7 forming a C-C linkage
638341
Rattus norvegicus
?
2.4.1.109
dolichyl phosphate D-mannose + human ribonuclease 2
recombinant wild-type RNase from E. coli is no substrate
638341
Rattus norvegicus
dolichyl phosphate + human ribonuclease 2-D-mannose
D-mannose is bound at Trp7 forming a C-C linkage
638341
Rattus norvegicus
?
2.4.1.109
dolichyl phosphate D-mannose + ribonuclease 2
C-mannosylation activity
638341
Rattus norvegicus
dolichyl phosphate + ribonuclease 2-D-mannose
-
-
-
-
2.4.1.109
dolichyl phosphate D-mannose + ribonuclease 2
C-mannosylation activity
638341
Rattus norvegicus
dolichyl phosphate + ribonuclease 2-D-mannose
D-mannose is bound at Trp7 forming a C-C linkage
638341
Rattus norvegicus
?
2.4.1.109
dolichyl phosphate D-mannose + ribonuclease 2
biosynthetic pathway
638341
Rattus norvegicus
dolichyl phosphate + ribonuclease 2-D-mannose
-
-
-
-
Temperature Optimum [C]
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
2.4.1.109
26
-
about
Rattus norvegicus
2.4.1.109
37
-
assay at
Rattus norvegicus
Temperature Stability [C]
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
2.4.1.109
26
-
22 h, stable
Rattus norvegicus
2.4.1.109
37
-
60 min, stable
Rattus norvegicus
2.4.1.109
95
-
inactivation
Rattus norvegicus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.4.1.109
7.2
-
assay at
Rattus norvegicus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.4.1.109
chymotrypsin
inactivation
Rattus norvegicus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.4.1.109
membrane
-
Rattus norvegicus
16020
-
2.4.1.109
microsome
-
Rattus norvegicus
-
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.4.1.109
Mg2+
-
Rattus norvegicus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.4.1.109
dolichyl phosphate D-mannose + ribonuclease 2
Rattus norvegicus
C-mannosylation activity
dolichyl phosphate + ribonuclease 2-D-mannose
-
-
-
2.4.1.109
dolichyl phosphate D-mannose + ribonuclease 2
Rattus norvegicus
biosynthetic pathway
dolichyl phosphate + ribonuclease 2-D-mannose
-
-
-
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.4.1.109
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
2.4.1.109
additional information
-
-
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.1.109
dolichyl phosphate D-mannose + human ribonuclease 2
whole protein or N-terminal dodecapeptide containing Trp7
638341
Rattus norvegicus
dolichyl phosphate + human ribonuclease 2-D-mannose
D-mannose is bound at Trp7 forming a C-C linkage
638341
Rattus norvegicus
?
2.4.1.109
dolichyl phosphate D-mannose + human ribonuclease 2
C-mannosylation activity
638341
Rattus norvegicus
dolichyl phosphate + human ribonuclease 2-D-mannose
D-mannose is bound at Trp7 forming a C-C linkage
638341
Rattus norvegicus
?
2.4.1.109
dolichyl phosphate D-mannose + human ribonuclease 2
no C-mannosylation activity of Trp7 when Trp10 is exchanged for Ala in the acceptor substrate
638341
Rattus norvegicus
dolichyl phosphate + human ribonuclease 2-D-mannose
D-mannose is bound at Trp7 forming a C-C linkage
638341
Rattus norvegicus
?
2.4.1.109
dolichyl phosphate D-mannose + human ribonuclease 2
recombinant wild-type RNase from E. coli is no substrate
638341
Rattus norvegicus
dolichyl phosphate + human ribonuclease 2-D-mannose
D-mannose is bound at Trp7 forming a C-C linkage
638341
Rattus norvegicus
?
2.4.1.109
dolichyl phosphate D-mannose + ribonuclease 2
C-mannosylation activity
638341
Rattus norvegicus
dolichyl phosphate + ribonuclease 2-D-mannose
-
-
-
-
2.4.1.109
dolichyl phosphate D-mannose + ribonuclease 2
C-mannosylation activity
638341
Rattus norvegicus
dolichyl phosphate + ribonuclease 2-D-mannose
D-mannose is bound at Trp7 forming a C-C linkage
638341
Rattus norvegicus
?
2.4.1.109
dolichyl phosphate D-mannose + ribonuclease 2
biosynthetic pathway
638341
Rattus norvegicus
dolichyl phosphate + ribonuclease 2-D-mannose
-
-
-
-
Temperature Optimum [C] (protein specific)
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
2.4.1.109
26
-
about
Rattus norvegicus
2.4.1.109
37
-
assay at
Rattus norvegicus
Temperature Stability [C] (protein specific)
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
2.4.1.109
26
-
22 h, stable
Rattus norvegicus
2.4.1.109
37
-
60 min, stable
Rattus norvegicus
2.4.1.109
95
-
inactivation
Rattus norvegicus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.4.1.109
7.2
-
assay at
Rattus norvegicus