BRENDA - Enzyme Database

The PMT gene family: protein O-glycosylation in Saccharomyces cerevisiae is vital

Gentzsch, M.; Tanner, W.; EMBO J. 15, 5752-5759 (1996)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
2.4.1.109
;
Saccharomyces cerevisiae
Engineering
EC Number
Amino acid exchange
Commentary
Organism
2.4.1.109
additional information
construction of all single, double and triple mutants of the genes PMT1-4 by gene disruption and crosses, characterization concerning growth, morphology, and their sensitivity to killer toxin K1, sorbitol dependence, caffeine and calcofluor white, overview; construction of all single, double and triple mutants of the genes PMT1-4 by gene disruption and crosses, characterization concerning growth, morphology, and their sensitivity to killer toxin K1, sorbitol dependence, caffeine and calcofluor white, overview; construction of all single, double and triple mutants of the genes PMT1-4 by gene disruption and crosses, characterization concerning growth, morphology, and their sensitivity to killer toxin K1, sorbitol dependence, caffeine and calcofluor white, overview; construction of all single, double and triple mutants of the genes PMT1-4 by gene disruption and crosses, characterization concerning growth, morphology, and their sensitivity to killer toxin K1, sorbitol dependence, caffeine and calcofluor white, overview
Saccharomyces cerevisiae
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.4.1.109
dolichyl phosphate D-mannose + protein
Saccharomyces cerevisiae
acceptor substrates: secretory proteins
dolichyl phosphate + O-D-mannosylprotein
-
-
-
2.4.1.109
dolichyl phosphate D-mannose + protein
Saccharomyces cerevisiae
production of cell-wall mannoproteins
dolichyl phosphate + O-D-mannosylprotein
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.4.1.109
Saccharomyces cerevisiae
P31382
PMT2
-
2.4.1.109
Saccharomyces cerevisiae
P33775
gene PMT1
-
2.4.1.109
Saccharomyces cerevisiae
P42934
gene PMT6
-
2.4.1.109
Saccharomyces cerevisiae
P46971
gene PMT4
-
2.4.1.109
Saccharomyces cerevisiae
P47190
gene PMT3
-
2.4.1.109
Saccharomyces cerevisiae
P52867
gene PMT5
-
2.4.1.109
Saccharomyces cerevisiae
Q06644
gene PMT7
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.1.109
dolichyl phosphate D-mannose + protein
the enzyme transfers mannosyl residues to the hydroxyl of serine or threonine residues
638339
Saccharomyces cerevisiae
dolichyl phosphate + O-D-mannosylprotein
-
638339
Saccharomyces cerevisiae
?
2.4.1.109
dolichyl phosphate D-mannose + protein
acceptor substrates: secretory proteins
638339
Saccharomyces cerevisiae
dolichyl phosphate + O-D-mannosylprotein
-
-
-
-
2.4.1.109
dolichyl phosphate D-mannose + protein
acceptor substrates: secretory proteins
638339
Saccharomyces cerevisiae
dolichyl phosphate + O-D-mannosylprotein
-
638339
Saccharomyces cerevisiae
?
2.4.1.109
dolichyl phosphate D-mannose + protein
production of cell-wall mannoproteins
638339
Saccharomyces cerevisiae
dolichyl phosphate + O-D-mannosylprotein
-
-
-
-
2.4.1.109
dolichyl phosphate D-mannose + Tyr-Ala-Thr-Ala-Val
wild-type and mutants
638339
Saccharomyces cerevisiae
dolichyl phosphate + O-D-mannosyl-Tyr-Ala-Thr-Ala-Val
-
638339
Saccharomyces cerevisiae
?
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.4.1.109
-
Saccharomyces cerevisiae
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
2.4.1.109
additional information
construction of all single, double and triple mutants of the genes PMT1-4 by gene disruption and crosses, characterization concerning growth, morphology, and their sensitivity to killer toxin K1, sorbitol dependence, caffeine and calcofluor white, overview
Saccharomyces cerevisiae
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.4.1.109
dolichyl phosphate D-mannose + protein
Saccharomyces cerevisiae
acceptor substrates: secretory proteins
dolichyl phosphate + O-D-mannosylprotein
-
-
-
2.4.1.109
dolichyl phosphate D-mannose + protein
Saccharomyces cerevisiae
production of cell-wall mannoproteins
dolichyl phosphate + O-D-mannosylprotein
-
-
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.1.109
dolichyl phosphate D-mannose + protein
the enzyme transfers mannosyl residues to the hydroxyl of serine or threonine residues
638339
Saccharomyces cerevisiae
dolichyl phosphate + O-D-mannosylprotein
-
638339
Saccharomyces cerevisiae
?
2.4.1.109
dolichyl phosphate D-mannose + protein
acceptor substrates: secretory proteins
638339
Saccharomyces cerevisiae
dolichyl phosphate + O-D-mannosylprotein
-
-
-
-
2.4.1.109
dolichyl phosphate D-mannose + protein
acceptor substrates: secretory proteins
638339
Saccharomyces cerevisiae
dolichyl phosphate + O-D-mannosylprotein
-
638339
Saccharomyces cerevisiae
?
2.4.1.109
dolichyl phosphate D-mannose + protein
production of cell-wall mannoproteins
638339
Saccharomyces cerevisiae
dolichyl phosphate + O-D-mannosylprotein
-
-
-
-
2.4.1.109
dolichyl phosphate D-mannose + Tyr-Ala-Thr-Ala-Val
wild-type and mutants
638339
Saccharomyces cerevisiae
dolichyl phosphate + O-D-mannosyl-Tyr-Ala-Thr-Ala-Val
-
638339
Saccharomyces cerevisiae
?