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Literature summary extracted from

  • Dotson, S.B.; Rush, J.S.; Ricketts, A.D.; Waechter, C.J.
    Mannosylphosphoryldolichol-mediated O-mannosylation of yeast glycoproteins: stereospecificity and recognition of the alpha-isoprene unit by a purified mannosyltransferase (1995), Arch. Biochem. Biophys., 316, 773-779.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.4.1.109 stable in solution with deoxycholate and CHAPS Saccharomyces cerevisiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.109 glycerol at 20% inhibitory Saccharomyces cerevisiae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.109 membrane
-
Saccharomyces cerevisiae 16020
-
2.4.1.109 microsome
-
Saccharomyces cerevisiae
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.109 dolichyl phosphate D-mannose + protein Saccharomyces cerevisiae
-
dolichyl phosphate + O-D-mannosylprotein
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.109 Saccharomyces cerevisiae
-
gene PMT1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.109 partial Saccharomyces cerevisiae
2.4.1.109 solubilization with 0.5% deoxycholate and 1.2% CHAPS Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.109 0.0035
-
partially purified enzyme Saccharomyces cerevisiae

Storage Stability

EC Number Storage Stability Organism
2.4.1.109 -80°C, partially purified enzyme, indefinitely stable Saccharomyces cerevisiae
2.4.1.109 23°C, partially purified enzyme, 2% glycerol, at least 12 h stable Saccharomyces cerevisiae
2.4.1.109 4-23°C, partially purified enzyme, stable for at least 1 week Saccharomyces cerevisiae
2.4.1.109 4°C, solubilized with 0.5% Triton X-100, loss of 80% activity within 5 days Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.109 dolichyl phosphate D-mannose + biotin-Tyr-Ala-Thr-Ala-Val-NH2
-
Saccharomyces cerevisiae dolichyl phosphate + O-D-mannosyl-N-biotinyl-Tyr-Ala-Thr-Ala-Val-NH2
-
?
2.4.1.109 dolichyl phosphate D-mannose + protein
-
Saccharomyces cerevisiae dolichyl phosphate + O-D-mannosylprotein
-
?
2.4.1.109 dolichyl phosphate D-mannose + protein the enzyme transfers mannosyl residues to the hydroxyl of serine or threonine residues Saccharomyces cerevisiae dolichyl phosphate + O-D-mannosylprotein
-
?
2.4.1.109 dolichyl phosphate D-mannose + protein preferred chain lengthin decreasing order: C100, C80, C55, C35 Saccharomyces cerevisiae dolichyl phosphate + O-D-mannosylprotein
-
?
2.4.1.109 dolichyl phosphate D-mannose + protein strictly stereospecific for the anomeric configuration of phosphoryl-linkage of the donor substrate, a saturated alpha-isoprene unit in the dolichyl moiety is required Saccharomyces cerevisiae dolichyl phosphate + O-D-mannosylprotein
-
?
2.4.1.109 dolichyl phosphate D-mannose + Tyr-Asn-Pro-Thr-Ser-Val-NH2
-
Saccharomyces cerevisiae dolichyl phosphate + O-alpha-D-mannosyl-Tyr-Asn-Pro-Thr-Ser-Val-NH2
-
?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.109 PMT
-
Saccharomyces cerevisiae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.109 30
-
assay at Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.109 7
-
assay at Saccharomyces cerevisiae

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.4.1.109 4.9
-
inactivation below pH 4.9 Saccharomyces cerevisiae