BRENDA - Enzyme Database

Protein O-glycosylation in Saccharomyces cerevisiae. Purification and characterization of the dolichyl-phosphate-D-mannose-protein O-D-mannosyltransferase

Strahl-Bolsinger, S.; Tanner, W.; Eur. J. Biochem. 196, 185-190 (1991)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.4.1.109
additional information
no inhibition by EDTA
Saccharomyces cerevisiae
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.4.1.109
0.4
-
dolichyl phosphate D-mannose
-
Saccharomyces cerevisiae
2.4.1.109
3.3
-
Tyr-Asn-Pro-Thr-Ser-Val
-
Saccharomyces cerevisiae
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.4.1.109
endoplasmic reticulum
-
Saccharomyces cerevisiae
5783
-
2.4.1.109
membrane
-
Saccharomyces cerevisiae
16020
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.4.1.109
Mg2+
2fold activation
Saccharomyces cerevisiae
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.4.1.109
92000
-
x * 92000, PMT1, SDS-PAGE; x * 92000, SDS-PAGE
Saccharomyces cerevisiae
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.4.1.109
dolichyl phosphate D-mannose + protein
Saccharomyces cerevisiae
production of cell-wall mannoproteins
dolichyl phosphate + O-D-mannosylprotein
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.4.1.109
Saccharomyces cerevisiae
-
-
-
Posttranslational Modification
EC Number
Posttranslational Modification
Commentary
Organism
2.4.1.109
glycoprotein
may contain 4 carbohydrate chains
Saccharomyces cerevisiae
Purification (Commentary)
EC Number
Commentary
Organism
2.4.1.109
partial
Saccharomyces cerevisiae
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
2.4.1.109
additional information
-
-
Saccharomyces cerevisiae
2.4.1.109
0.0000677
-
partially purified enzyme
Saccharomyces cerevisiae
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.1.109
dolichyl phosphate D-mannose + Lys-Pro-Ser-Gly-Tyr
-
638331
Saccharomyces cerevisiae
dolichyl phosphate + O-D-mannosyl-Lys-Pro-Ser-Gly-Tyr
-
638331
Saccharomyces cerevisiae
?
2.4.1.109
dolichyl phosphate D-mannose + Lys-Pro-Thr-Gly-Tyr
-
638331
Saccharomyces cerevisiae
dolichyl phosphate + O-D-mannosyl-Lys-Pro-Thr-Gly-Tyr
-
638331
Saccharomyces cerevisiae
?
2.4.1.109
dolichyl phosphate D-mannose + Lys-Pro-Thr-Pro-Tyr
-
638331
Saccharomyces cerevisiae
dolichyl phosphate + O-D-mannosyl-Lys-Pro-Thr-Pro-Tyr
-
638331
Saccharomyces cerevisiae
?
2.4.1.109
dolichyl phosphate D-mannose + Pro-Thr-Val
-
638331
Saccharomyces cerevisiae
dolichyl phosphate + O-D-mannosyl-Pro-Thr-Val
-
638331
Saccharomyces cerevisiae
?
2.4.1.109
dolichyl phosphate D-mannose + Pro-Tyr-Thr-Val
-
638331
Saccharomyces cerevisiae
dolichyl phosphate + O-D-mannosyl-Pro-Tyr-Thr-Val
-
638331
Saccharomyces cerevisiae
?
2.4.1.109
dolichyl phosphate D-mannose + protein
-
638331
Saccharomyces cerevisiae
dolichyl phosphate + O-D-mannosylprotein
-
638331
Saccharomyces cerevisiae
?
2.4.1.109
dolichyl phosphate D-mannose + protein
the enzyme transfers mannosyl residues to the hydroxyl of serine or threonine residues
638331
Saccharomyces cerevisiae
dolichyl phosphate + O-D-mannosylprotein
-
638331
Saccharomyces cerevisiae
?
2.4.1.109
dolichyl phosphate D-mannose + protein
acidic amino acids strongly inhibit acceptor activity, as do glycine and proline residues as amino-terminal and carboxy-terminal neighbours
638331
Saccharomyces cerevisiae
dolichyl phosphate + O-D-mannosylprotein
-
638331
Saccharomyces cerevisiae
?
2.4.1.109
dolichyl phosphate D-mannose + protein
production of cell-wall mannoproteins
638331
Saccharomyces cerevisiae
dolichyl phosphate + O-D-mannosylprotein
-
-
-
-
2.4.1.109
dolichyl phosphate D-mannose + Tyr-Asn-Leu-Thr-Ser-Val
-
638331
Saccharomyces cerevisiae
dolichyl phosphate + O-D-mannosyl-Tyr-Asn-Leu-Thr-Ser-Val
-
638331
Saccharomyces cerevisiae
?
2.4.1.109
dolichyl phosphate D-mannose + Tyr-Asn-Pro-Thr-Ser-Val
-
638331
Saccharomyces cerevisiae
dolichyl phosphate + O-D-mannosyl-Tyr-Asn-Pro-Thr-Ser-Val
-
638331
Saccharomyces cerevisiae
?
Subunits
EC Number
Subunits
Commentary
Organism
2.4.1.109
?
x * 92000, PMT1, SDS-PAGE; x * 92000, SDS-PAGE
Saccharomyces cerevisiae
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.4.1.109
7.5
-
-
Saccharomyces cerevisiae
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.4.1.109
additional information
no inhibition by EDTA
Saccharomyces cerevisiae
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.4.1.109
0.4
-
dolichyl phosphate D-mannose
-
Saccharomyces cerevisiae
2.4.1.109
3.3
-
Tyr-Asn-Pro-Thr-Ser-Val
-
Saccharomyces cerevisiae
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.4.1.109
endoplasmic reticulum
-
Saccharomyces cerevisiae
5783
-
2.4.1.109
membrane
-
Saccharomyces cerevisiae
16020
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.4.1.109
Mg2+
2fold activation
Saccharomyces cerevisiae
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.4.1.109
92000
-
x * 92000, PMT1, SDS-PAGE; x * 92000, SDS-PAGE
Saccharomyces cerevisiae
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.4.1.109
dolichyl phosphate D-mannose + protein
Saccharomyces cerevisiae
production of cell-wall mannoproteins
dolichyl phosphate + O-D-mannosylprotein
-
-
-
Posttranslational Modification (protein specific)
EC Number
Posttranslational Modification
Commentary
Organism
2.4.1.109
glycoprotein
may contain 4 carbohydrate chains
Saccharomyces cerevisiae
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.4.1.109
partial
Saccharomyces cerevisiae
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
2.4.1.109
additional information
-
-
Saccharomyces cerevisiae
2.4.1.109
0.0000677
-
partially purified enzyme
Saccharomyces cerevisiae
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.1.109
dolichyl phosphate D-mannose + Lys-Pro-Ser-Gly-Tyr
-
638331
Saccharomyces cerevisiae
dolichyl phosphate + O-D-mannosyl-Lys-Pro-Ser-Gly-Tyr
-
638331
Saccharomyces cerevisiae
?
2.4.1.109
dolichyl phosphate D-mannose + Lys-Pro-Thr-Gly-Tyr
-
638331
Saccharomyces cerevisiae
dolichyl phosphate + O-D-mannosyl-Lys-Pro-Thr-Gly-Tyr
-
638331
Saccharomyces cerevisiae
?
2.4.1.109
dolichyl phosphate D-mannose + Lys-Pro-Thr-Pro-Tyr
-
638331
Saccharomyces cerevisiae
dolichyl phosphate + O-D-mannosyl-Lys-Pro-Thr-Pro-Tyr
-
638331
Saccharomyces cerevisiae
?
2.4.1.109
dolichyl phosphate D-mannose + Pro-Thr-Val
-
638331
Saccharomyces cerevisiae
dolichyl phosphate + O-D-mannosyl-Pro-Thr-Val
-
638331
Saccharomyces cerevisiae
?
2.4.1.109
dolichyl phosphate D-mannose + Pro-Tyr-Thr-Val
-
638331
Saccharomyces cerevisiae
dolichyl phosphate + O-D-mannosyl-Pro-Tyr-Thr-Val
-
638331
Saccharomyces cerevisiae
?
2.4.1.109
dolichyl phosphate D-mannose + protein
-
638331
Saccharomyces cerevisiae
dolichyl phosphate + O-D-mannosylprotein
-
638331
Saccharomyces cerevisiae
?
2.4.1.109
dolichyl phosphate D-mannose + protein
the enzyme transfers mannosyl residues to the hydroxyl of serine or threonine residues
638331
Saccharomyces cerevisiae
dolichyl phosphate + O-D-mannosylprotein
-
638331
Saccharomyces cerevisiae
?
2.4.1.109
dolichyl phosphate D-mannose + protein
acidic amino acids strongly inhibit acceptor activity, as do glycine and proline residues as amino-terminal and carboxy-terminal neighbours
638331
Saccharomyces cerevisiae
dolichyl phosphate + O-D-mannosylprotein
-
638331
Saccharomyces cerevisiae
?
2.4.1.109
dolichyl phosphate D-mannose + protein
production of cell-wall mannoproteins
638331
Saccharomyces cerevisiae
dolichyl phosphate + O-D-mannosylprotein
-
-
-
-
2.4.1.109
dolichyl phosphate D-mannose + Tyr-Asn-Leu-Thr-Ser-Val
-
638331
Saccharomyces cerevisiae
dolichyl phosphate + O-D-mannosyl-Tyr-Asn-Leu-Thr-Ser-Val
-
638331
Saccharomyces cerevisiae
?
2.4.1.109
dolichyl phosphate D-mannose + Tyr-Asn-Pro-Thr-Ser-Val
-
638331
Saccharomyces cerevisiae
dolichyl phosphate + O-D-mannosyl-Tyr-Asn-Pro-Thr-Ser-Val
-
638331
Saccharomyces cerevisiae
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.4.1.109
?
x * 92000, PMT1, SDS-PAGE; x * 92000, SDS-PAGE
Saccharomyces cerevisiae
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.4.1.109
7.5
-
-
Saccharomyces cerevisiae