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Literature summary extracted from

  • Ju, T.; Cummings, R.D.; Canfield, W.M.
    Purification, characterization, and subunit structure of rat core 1 beta1,3-galactosyltransferase (2002), J. Biol. Chem., 277, 169-177.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.122 UDP slight inhibition Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.122 0.63
-
 UDP-galactose with N-acetyl-D-galactosaminyl-alpha-phenyl Rattus norvegicus
2.4.1.122 0.76
-
 N-acetyl-alpha-D-galactosaminyl-phenyl
-
 Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.122 membrane associated Rattus norvegicus 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.122 Mn2+ required Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.122 84000 86000 gel filtration Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.122 UDP-galactose + glycoprotein N-acetyl-D-galactosamine Rattus norvegicus key enzyme in biosynthesis of core 1 O-glycans UDP + glycoprotein D-galactosyl-1,3-N-acetyl-D-galactosamine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.122 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.122 affinity chromatography on immobilized asialo-bovine submaxillary gland mucin Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.122 liver high content Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.122 0.000042
-
 liver Rattus norvegicus
2.4.1.122 3.43
-
 purified enzyme Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.122 UDP-galactose + asialo bovine submaxillary mucin
-
 Rattus norvegicus UDP + beta-D-galactosyl-1,3-N-acetylgalactosyl-asialo bovine submaxillary mucin
-
 ?
2.4.1.122 UDP-galactose + glycoprotein N-acetyl-D-galactosamine linked to serine or threonine of the peptide/protein Rattus norvegicus UDP + glycoprotein D-galactosyl-1,3-N-acetyl-D-galactosamine i.e. core 1 structure ?
2.4.1.122 UDP-galactose + glycoprotein N-acetyl-D-galactosamine key enzyme in biosynthesis of core 1 O-glycans Rattus norvegicus UDP + glycoprotein D-galactosyl-1,3-N-acetyl-D-galactosamine
-
 ?
2.4.1.122 UDP-galactose + N-acetyl-alpha-D-galactosaminyl-phenyl
-
 Rattus norvegicus UDP + beta-D-galactosyl-1,3-N-acetyl-alpha-D-galactosaminyl-phenyl
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.122 monomer 1 * 42000-43000, SDS-PAGE and amino acid sequence determination Rattus norvegicus

Synonyms

EC Number Synonyms Comment Organism
2.4.1.122 core 1 beta3-Gal-T
-
 Rattus norvegicus
2.4.1.122 UDP-Gal:GalNAc-alpha-Ser/Thr beta3-galactosyltransferase
-
 Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.122 37
-
 assay at Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.122 6.5
-
 assay at Rattus norvegicus