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Literature summary extracted from

  • Mendicino, J.; Sivakami, S.; Davila, M.; Chandrasekaran, E.V.
    Purification and properties of UDP-Gal:N-acetylgalactosaminide mucin: beta 1,3-galactosyltransferase from swine trachea mucosa (1982), J. Biol. Chem., 257, 3987-3994.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.122 Triton X-100 activation and stabilization, unstable without Sus scrofa

General Stability

EC Number General Stability Organism
2.4.1.122 stabilization by Triton X-100 or Nonidet P-40 required, unstable without Sus scrofa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.122 0.00035
-
 asialo-mucin of Cowper's gland
-
 Sus scrofa
2.4.1.122 0.02
-
 UDP-galactose
-
 Sus scrofa
2.4.1.122 0.09
-
 asialo-mucin of Cowper's gland calculated on the basis of terminal N-galactosaminyl residues Sus scrofa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.122 endoplasmic reticulum
-
 Sus scrofa 5783
-
2.4.1.122 Golgi apparatus major part Sus scrofa 5794
-
2.4.1.122 membrane inner surface Sus scrofa 16020
-
2.4.1.122 microsome
-
 Sus scrofa
-
-
2.4.1.122 additional information subcellular distribution Sus scrofa
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.122 Mn2+
-
 Sus scrofa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.122 82000
-
 1 * 82000, SDS-PAGE Sus scrofa
2.4.1.122 90000
-
 gel filtration in the presence of Triton X-100 Sus scrofa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.122 UDP-galactose + N-acetylgalactosaminide mucin Sus scrofa participates in synthesis of the core portion of O-serine- and O-threonine-linked oligosaccharides in respiratory acidic mucins UDP + beta-D-galactosyl-1,3-N-acetylgalactosaminide mucin
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.122 Sus scrofa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.122 affinity chromatography on Sepharose 4B containing covalently bound mucin substrate Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.122 tracheal mucosa
-
 Sus scrofa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.122 0.0194
-
 Golgi membranes Sus scrofa
2.4.1.122 0.81
-
 purified enzyme Sus scrofa

Storage Stability

EC Number Storage Stability Organism
2.4.1.122 3°C, 0.1% Triton X-100 and 0.1 mg/ml albumin, at least 3 months Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.122 additional information poor substrates are low-molecular weight acceptors, e.g. N-acetylgalactosamine Sus scrofa ?
-
 ?
2.4.1.122 additional information no acceptor substrates: type A and type 0 blood group glycoprotein, ovomucoid, ovalbumin, and fetuin devoid of sialic acid and galactose Sus scrofa ?
-
 ?
2.4.1.122 additional information terminal alpha-2,6-linked sialic acid residues inhibit the enzyme Sus scrofa ?
-
 ?
2.4.1.122 UDP-galactose + asialo-mucin of Cowper's gland
-
 Sus scrofa UDP + beta-D-galactosyl-1,3-N-acetylgalactosyl-asialo-mucin of Cowper's gland
-
 ?
2.4.1.122 UDP-galactose + glycoprotein N-acetyl-D-galactosamine high specificity for N-acetylgalactosaminyl residues linked to serine or threonine Sus scrofa UDP + glycoprotein D-galactosyl-1,3-N-acetyl-D-galactosamine
-
 ?
2.4.1.122 UDP-galactose + glycoprotein N-acetyl-D-galactosamine most active acceptor: macromolecular mucin glycopeptides with free N-acetylgalactosyl residues linked to polypeptide chain Sus scrofa UDP + glycoprotein D-galactosyl-1,3-N-acetyl-D-galactosamine
-
 ?
2.4.1.122 UDP-galactose + glycoprotein N-acetyl-D-galactosamine glycopeptides with less than 5 amino acids containing terminal N-acetylgalactosaminyl residues Sus scrofa UDP + glycoprotein D-galactosyl-1,3-N-acetyl-D-galactosamine
-
 ?
2.4.1.122 UDP-galactose + glycoprotein N-acetyl-D-galactosamine glycosyl acceptor specificity study Sus scrofa UDP + glycoprotein D-galactosyl-1,3-N-acetyl-D-galactosamine
-
 ?
2.4.1.122 UDP-galactose + N-acetyl-D-galactosamine
-
 Sus scrofa UDP + beta-D-galactosyl-1,3-N-acetyl-D-galactosamine
-
 ?
2.4.1.122 UDP-galactose + N-acetylgalactosaminide mucin
-
 Sus scrofa UDP + beta-D-galactosyl-1,3-N-acetylgalactosaminide mucin
-
 ?
2.4.1.122 UDP-galactose + N-acetylgalactosaminide mucin participates in synthesis of the core portion of O-serine- and O-threonine-linked oligosaccharides in respiratory acidic mucins Sus scrofa UDP + beta-D-galactosyl-1,3-N-acetylgalactosaminide mucin
-
 ?
2.4.1.122 UDP-galactose + N-acetylgalactosaminitol
-
 Sus scrofa UDP + beta-D-galactosyl-(1,3)-N-acetylgalactosaminitol
-
 ?
2.4.1.122 UDP-galactose + p-nitrophenyl-beta-N-acetylgalactosamide
-
 Sus scrofa UDP + beta-D-galactosyl-1,3-N-acetylgalactosyl-p-nitrophenyl
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.122 monomer 1 * 82000, SDS-PAGE Sus scrofa

Synonyms

EC Number Synonyms Comment Organism
2.4.1.122 UDP-Gal:N-acetylgalactosaminide mucin:beta1,3-galactosyltransferase
-
 Sus scrofa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.122 37
-
 assay at Sus scrofa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.122 6.9
-
-
 Sus scrofa

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.122 5.5 7.9
-
 Sus scrofa