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Literature summary extracted from

  • Martin, R.C.; Mok, M.C.; Mok, D.W.S.
    Protein processing and auxin response in transgenic tobacco harboring a putative cDNA of zeatin O-xylosyltransferase from Phaseolus vulgaris (1997), Plant J., 12, 305-312.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.2.40 expression in Escherichia coli and in Spodoptera frugiperda Sf21 cells, via baculovirus infection, produced an insoluble protein, not successful Phaseolus vulgaris
2.4.2.40 in vitro transcription and translation, posttranslational processing by bean endosperm extract Phaseolus vulgaris

Protein Variants

EC Number Protein Variants Comment Organism
2.4.2.40 additional information construction of transgenic tobacco plants via Agrobacterium tumefaciens infection, functional expression under control of the CaMV35S promotor in seedlings and leaves, transgenic plants are more sensitive to auxin than wild-type plants, phenotypic alterations are leaf chlorosis, restriction of root elongation and eventual cessation of growth Phaseolus vulgaris

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.2.40 cytosol
-
 Phaseolus vulgaris 5829
-
2.4.2.40 nucleus
-
 Phaseolus vulgaris 5634
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.2.40 additional information
-
 an inactive form of MW about 70 kDa is detected by immunostaining in vegetative tissue prior to posttranslational processing of the protein Phaseolus vulgaris
2.4.2.40 50000
-
-
 Phaseolus vulgaris

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.40 Phaseolus vulgaris
-
 cv. Great Northern
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.2.40 endosperm
-
 Phaseolus vulgaris
-
2.4.2.40 seed
-
 Phaseolus vulgaris
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.40 UDP-D-xylose + zeatin
-
 Phaseolus vulgaris UDP + O-beta-D-xylosylzeatin
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.2.40 monomer 1 * 50000, SDS-PAGE Phaseolus vulgaris

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.2.40 27
-
 assay at Phaseolus vulgaris