BRENDA - Enzyme Database

PMT3 and PMT4, two new members of the protein-O-mannosyltransferase gene family of Saccharomyces cerevisiae

Immervoll, T.; Gentzsch, M.; Tanner, W.; Yeast 11, 1345-1351 (1995)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
2.4.1.109
; DNA sequence determination
Saccharomyces cerevisiae
Engineering
EC Number
Amino acid exchange
Commentary
Organism
2.4.1.109
additional information
homozygous diploid double disruption mutants of PMT1 and PMT2 lead to reduced enzyme activity and a severe defect in sporulation, lethal phenotype when combined with a disruption mutation in dolichyl-phosphate-mannose-glycolipid alpha-mannosyltransferase, EC 2.4.1.130, genes; PMT3 and 4 gene disruption does not alter enzyme activity; PMT3 and 4 gene disruption does not alter enzyme activity
Saccharomyces cerevisiae
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.4.1.109
membrane
-
Saccharomyces cerevisiae
16020
-
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.4.1.109
dolichyl phosphate D-mannose + protein
Saccharomyces cerevisiae
production of cell-wall mannoproteins
dolichyl phosphate + O-D-mannosylprotein
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.4.1.109
Saccharomyces cerevisiae
P46971
gene PMT4
-
2.4.1.109
Saccharomyces cerevisiae
P47190
gene PMT3
-
2.4.1.109
Saccharomyces cerevisiae
-
gene PMT1; gene PMT2
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.1.109
dolichyl phosphate D-mannose + protein
-
638312
Saccharomyces cerevisiae
dolichyl phosphate + O-D-mannosylprotein
-
638312
Saccharomyces cerevisiae
?
2.4.1.109
dolichyl phosphate D-mannose + protein
the enzyme transfers mannosyl residues to the hydroxyl of serine or threonine residues
638312
Saccharomyces cerevisiae
dolichyl phosphate + O-D-mannosylprotein
-
638312
Saccharomyces cerevisiae
?
2.4.1.109
dolichyl phosphate D-mannose + protein
production of cell-wall mannoproteins
638312
Saccharomyces cerevisiae
dolichyl phosphate + O-D-mannosylprotein
-
-
-
-
2.4.1.109
additional information
-
638312
Saccharomyces cerevisiae
?
-
-
-
-
2.4.1.109
additional information
PMT3 behaves as a dolichyl-phosphate-mannose-glycolipid alpha-mannosyltransferase, EC 2.4.1.130
638312
Saccharomyces cerevisiae
?
-
-
-
-
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.4.1.109
-
Saccharomyces cerevisiae
2.4.1.109
DNA sequence determination
Saccharomyces cerevisiae
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
2.4.1.109
additional information
homozygous diploid double disruption mutants of PMT1 and PMT2 lead to reduced enzyme activity and a severe defect in sporulation, lethal phenotype when combined with a disruption mutation in dolichyl-phosphate-mannose-glycolipid alpha-mannosyltransferase, EC 2.4.1.130, genes
Saccharomyces cerevisiae
2.4.1.109
additional information
PMT3 and 4 gene disruption does not alter enzyme activity
Saccharomyces cerevisiae
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.4.1.109
membrane
-
Saccharomyces cerevisiae
16020
-
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.4.1.109
dolichyl phosphate D-mannose + protein
Saccharomyces cerevisiae
production of cell-wall mannoproteins
dolichyl phosphate + O-D-mannosylprotein
-
-
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.1.109
dolichyl phosphate D-mannose + protein
-
638312
Saccharomyces cerevisiae
dolichyl phosphate + O-D-mannosylprotein
-
638312
Saccharomyces cerevisiae
?
2.4.1.109
dolichyl phosphate D-mannose + protein
the enzyme transfers mannosyl residues to the hydroxyl of serine or threonine residues
638312
Saccharomyces cerevisiae
dolichyl phosphate + O-D-mannosylprotein
-
638312
Saccharomyces cerevisiae
?
2.4.1.109
dolichyl phosphate D-mannose + protein
production of cell-wall mannoproteins
638312
Saccharomyces cerevisiae
dolichyl phosphate + O-D-mannosylprotein
-
-
-
-
2.4.1.109
additional information
-
638312
Saccharomyces cerevisiae
?
-
-
-
-
2.4.1.109
additional information
PMT3 behaves as a dolichyl-phosphate-mannose-glycolipid alpha-mannosyltransferase, EC 2.4.1.130
638312
Saccharomyces cerevisiae
?
-
-
-
-