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Literature summary extracted from

  • Toki, D.; Sarkar, M.; Yip, B.; Reck, F.; Joziasse, D.; Fukuda, M.; Schachter, H.; Brockhausen, I.
    Expression of stable human O-glycan core 2 beta-1,6-N-acetylglycosaminyltransferase in Sf9 insect cells (1997), Biochem. J., 325, 63-69.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.102 expression in Spodoptera frugiperda Sf9 insect cells via baculovirus infection, truncated enzyme form Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.102 T60A site-directed mutagenesis, highly reduced activity Homo sapiens
2.4.1.102 T60A/T97A site-directed mutagenesis, no activity, no secretion of Sf9 cells Homo sapiens
2.4.1.102 T97A site-directed mutagenesis, 50% reduced activity Homo sapiens

General Stability

EC Number General Stability Organism
2.4.1.102 freeze-drying and resuspension in 10 mM Tris-HCl, pH 8.0, 1 mM EDTA, 95% remaining activity Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.102 tunicamycin inactivation; prevents secretion of recombinant enzyme from Sf9 cells Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.102 extracellular secretion into medium of the recombinant enzyme from Sf9 cells Homo sapiens
-
-

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.102 Homo sapiens
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.4.1.102 glycoprotein N-glycans at both glycosylation sites Homo sapiens
2.4.1.102 glycoprotein binds concanavalin A Homo sapiens

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.102 partially, recombinant from Sf9 insect cells Homo sapiens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.102 additional information
-
-
 Homo sapiens

Storage Stability

EC Number Storage Stability Organism
2.4.1.102 -70°C, crude enzyme extract, stable up to 1 year Homo sapiens
2.4.1.102 4°C, stable in solution or after lyophilization for several months Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.102 UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,3-N-acetyl-D-galactosaminyl-R
-
 Homo sapiens UDP + beta-D-galactosyl-1,3-(N-acetyl-beta-D-glucosaminyl-1,6)-N-acetyl-D-galactosaminyl-R i.e. core class 2 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.102 37
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.102 7
-
-
 Homo sapiens