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Literature summary extracted from

  • Peterbauer, T.; Richter, A.
    Galactosylononitol and stachyose synthesis in seeds of adzuki bean. Purification and characterization of stachyose synthase (1998), Plant Physiol., 117, 165-172.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.67 Cu2+
-
 Vigna angularis
2.4.1.67 D-ononitol competitive inhibition when the enzyme is incubated with both raffinose and D-ononitol simultaneously Vigna angularis
2.4.1.67 Fe2+
-
 Vigna angularis
2.4.1.67 Mn2+
-
 Vigna angularis
2.4.1.67 raffinose competitive inhibition when the enzyme is incubated with both raffinose and D-ononitol simultaneously Vigna angularis
2.4.1.67 Zn2+
-
 Vigna angularis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.67 1.8
-
 1-alpha-D-galactosyl-myo-inositol with raffinose Vigna angularis
2.4.1.67 2.4
-
 raffinose with 1-alpha-D-galactosyl-myo-inositol at 1.1 mM Vigna angularis
2.4.1.67 5.5
-
 myo-inositol with 10 mM 1-alpha-D-galactosyl-myo-inositol in the exchange reaction Vigna angularis
2.4.1.67 13.2
-
 raffinose with galactosylononitol at nonsaturating level Vigna angularis
2.4.1.67 31.3
-
 alpha-D-galactosyl-D-ononitol
-
 Vigna angularis
2.4.1.67 38.6
-
 raffinose with 1-alpha-D-galactosyl-myo-inositol at nonsaturating level Vigna angularis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.67 dithiothreitol activation Vigna angularis
2.4.1.67 EDTA slight stimulation Vigna angularis
2.4.1.67 Mg2+ slight stimulation Vigna angularis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.67 additional information
-
 enzyme forms a 283 kDa aggregate during gel filtration step Vigna angularis
2.4.1.67 90100
-
 1 * 90100, SDS-PAGE Vigna angularis
2.4.1.67 110000
-
 gel filtration Vigna angularis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.67 1-alpha-D-galactosyl-myo-inositol + D-ononitol Vigna angularis biosynthesis of galactosylononitol myo-inositol + alpha-D-galactosyl-D-ononitol
-
 ?
2.4.1.67 1-alpha-D-galactosyl-myo-inositol + raffinose Vigna angularis
-
 myo-inositol + stachyose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.67 Vigna angularis
-
 adzuki bean
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.67
-
 Vigna angularis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.67 seed
-
 Vigna angularis
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.67 0.67
-
 purified enzyme Vigna angularis
2.4.1.67 1.3
-
 purified enzyme, substrates: 1-alpha-D-galactosyl-myo-inositol + D-ononitol Vigna angularis

Storage Stability

EC Number Storage Stability Organism
2.4.1.67 -196°C, stored in liquid N2, stable for at least 4 months Vigna angularis
2.4.1.67 -20°C, loss of 85% activity within 4 months Vigna angularis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.67 1-alpha-D-galactosyl-myo-inositol + D-ononitol ononitol is 1-D-4-O-methyl-myo-inositol Vigna angularis myo-inositol + alpha-D-galactosyl-D-ononitol
-
 r
2.4.1.67 1-alpha-D-galactosyl-myo-inositol + D-ononitol biosynthesis of galactosylononitol Vigna angularis myo-inositol + alpha-D-galactosyl-D-ononitol
-
 ?
2.4.1.67 1-alpha-D-galactosyl-myo-inositol + myo-inositol exchange reaction Vigna angularis myo-inositol + 1-alpha-D-galactosyl-myo-inositol
-
 r
2.4.1.67 1-alpha-D-galactosyl-myo-inositol + myo-inositol several isomeric inositols and inositol O-methyl-ethers are substrates, some are inactive, overview Vigna angularis myo-inositol + 1-alpha-D-galactosyl-myo-inositol
-
 r
2.4.1.67 1-alpha-D-galactosyl-myo-inositol + myo-inositol 2.25fold increased activity compared to raffinose Vigna angularis myo-inositol + 1-alpha-D-galactosyl-myo-inositol
-
 r
2.4.1.67 1-alpha-D-galactosyl-myo-inositol + raffinose
-
 Vigna angularis myo-inositol + stachyose
-
 ?
2.4.1.67 1-alpha-D-galactosyl-myo-inositol + raffinose i.e. galactinol Vigna angularis myo-inositol + stachyose
-
 ?
2.4.1.67 1-alpha-D-galactosyl-myo-inositol + sequoyitol sequoyitol is 5-O-methyl-myo-inositol Vigna angularis myo-inositol + alpha-D-galactosyl-sequoyitol
-
 ?
2.4.1.67 1-alpha-D-galactosyl-myo-inositol + sequoyitol 1.3fold increased activity compared to raffinose Vigna angularis myo-inositol + alpha-D-galactosyl-sequoyitol
-
 ?
2.4.1.67 alpha-D-galactosyl-D-ononitol + raffinose
-
 Vigna angularis D-ononitol + stachyose
-
 ?
2.4.1.67 additional information substrate specificity, overview Vigna angularis ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.67 monomer 1 * 90100, SDS-PAGE Vigna angularis

Synonyms

EC Number Synonyms Comment Organism
2.4.1.67 stachyose synthase
-
 Vigna angularis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.67 35
-
-
 Vigna angularis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.4.1.67 23 35 half-maximal activity at 23°C Vigna angularis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.67 additional information
-
 several active forms with pI values between 4.7 and 5.0 Vigna angularis
2.4.1.67 6.5 7
-
 Vigna angularis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.4.1.67 additional information
-
 unstable at low pH-values Vigna angularis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.4.1.67 17.9
-
 raffinose competitive against D-ononitol Vigna angularis
2.4.1.67 18.7
-
 D-ononitol competitive against raffinose Vigna angularis