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Literature summary extracted from

  • Gaudreault, P.R.; Webb, J.A.
    Stachyose synthesis in leaves of Cucurbita pepo (1981), Phytochemistry, 20, 2629-2633.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.67 additional information no effect of EDTA Cucurbita pepo

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.67 Ag+ weak Cucurbita pepo
2.4.1.67 Ca2+ weak Cucurbita pepo
2.4.1.67 Co2+
-
 Cucurbita pepo
2.4.1.67 Cu2+ weak Cucurbita pepo
2.4.1.67 Hg2+
-
 Cucurbita pepo
2.4.1.67 melibiose i.e. 6-O-alpha-D-galactosyl-alpha-D-glucose, non-competitive to raffinose, weak Cucurbita pepo
2.4.1.67 Mg2+ weak Cucurbita pepo
2.4.1.67 Mn2+
-
 Cucurbita pepo
2.4.1.67 additional information no inhibitors: 5 mM EDTA, 3 mM MoO42-, K+, Na+, Fe3+, stachyose, galactose Cucurbita pepo
2.4.1.67 myo-inositol strong, competitive Cucurbita pepo
2.4.1.67 Ni2+
-
 Cucurbita pepo
2.4.1.67 Tris buffer 0.1 M Cucurbita pepo
2.4.1.67 Zn2+
-
 Cucurbita pepo

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.67 0.33
-
 1-alpha-D-galactosyl-myo-inositol hydrolysis Cucurbita pepo
2.4.1.67 4.6
-
 raffinose + 1-alpha-D-galactosyl-myo-inositol Cucurbita pepo
2.4.1.67 5.2
-
 melibiose + 1-alpha-D-galactosyl-myo-inositol Cucurbita pepo
2.4.1.67 7.7
-
 1-alpha-D-galactosyl-myo-inositol + raffinose Cucurbita pepo

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.67 cytoplasm
-
 Cucurbita pepo 5737
-

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.67 Cucurbita pepo
-
 var. melopepo f. torticolis Bailey
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.67 partial Cucurbita pepo

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.67 leaf mature Cucurbita pepo
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.67 0.0185
-
 partially purified enzyme Cucurbita pepo

Storage Stability

EC Number Storage Stability Organism
2.4.1.67 4°C, 0.1 M sodium phosphate buffer, 20 mM 2-mercaptoethanol, up to 45 days Cucurbita pepo

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.67 1-alpha-D-galactosyl-myo-inositol + H2O hydrolysis Cucurbita pepo myo-inositol + D-galactose
-
 ?
2.4.1.67 1-alpha-D-galactosyl-myo-inositol + melibiose 10% activity compared to raffinose Cucurbita pepo myo-inositol + alpha-D-galactosyl-6-O-D-galactosyl-alpha-D-glucose i.e. manninotriose ir
2.4.1.67 1-alpha-D-galactosyl-myo-inositol + raffinose i.e. galactinol Cucurbita pepo myo-inositol + stachyose
-
 r
2.4.1.67 additional information high donor specificity: UDP-galactose or melibiose are no galactosyl-donors Cucurbita pepo ?
-
 ?
2.4.1.67 additional information no substrates are sucrose, maltose, fructose, cellobiose, gentiobiose, melizitose, trehalose Cucurbita pepo ?
-
 ?
2.4.1.67 additional information no substrates: lactose, glucose, maltotriose, manninotriose Cucurbita pepo ?
-
 ?
2.4.1.67 p-nitrophenyl-alpha-D-galactopyranoside + raffinose about 10% activity compared to 1-alpha-D-galactosyl-myo-inositol Cucurbita pepo p-nitrophenol + stachyose
-
 r

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.67 30
-
 assay at Cucurbita pepo

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.67 6.5 6.9 plateau Cucurbita pepo

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.67 5.4 7.6 about half-maximal activity at pH 5.4 and pH 7.6 Cucurbita pepo

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.4.1.67 2
-
 myo-inositol
-
 Cucurbita pepo