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Literature summary extracted from

  • Goldraij, A.; Curtino, J.A.
    M-glycogenin, the protein moiety of Neurospora crassa proteoglycogen, is an auto- and transglucosylating enzyme (1996), Biochem. Biophys. Res. Commun., 227, 909-914.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.4.1.186 loss of activity on Q-Sepharose during ion exchange chromatography Neurospora crassa

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.186 EDTA
-
 Neurospora crassa
2.4.1.186 phosphate slight inhibition at 100 mM Neurospora crassa
2.4.1.186 UDP inhibits both autoglucosylation and glucosylation of exogenous acceptor Neurospora crassa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.186 Mg2+ can not substitute Mn2+ Neurospora crassa
2.4.1.186 Mn2+ dependent on, also required for transglucosylation Neurospora crassa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.186 31000
-
 SDS-PAGE Neurospora crassa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.186 UDP-glucose + glycogenin Escherichia coli enzyme forms the protein part of proteoglycogen UDP + glucosylated glycogenin
-
 ?
2.4.1.186 UDP-glucose + glycogenin Neurospora crassa enzyme forms the protein part of proteoglycogen UDP + glucosylated glycogenin
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.186 Escherichia coli
-
-
-
2.4.1.186 Neurospora crassa
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.4.1.186 glycoprotein autoglycosylation Escherichia coli
2.4.1.186 glycoprotein autoglycosylation Neurospora crassa

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.186 separation from proteoglycogen Neurospora crassa

Reaction

EC Number Reaction Comment Organism Reaction ID
2.4.1.186 UDP-alpha-D-glucose + glycogenin = UDP + alpha-D-glucosylglycogenin independent active sites for glucosylation of exogenous and self-acceptors Neurospora crassa
a
2.4.1.186 UDP-alpha-D-glucose + glycogenin = UDP + alpha-D-glucosylglycogenin highly conserved protein Escherichia coli
a
2.4.1.186 UDP-alpha-D-glucose + glycogenin = UDP + alpha-D-glucosylglycogenin highly conserved protein Neurospora crassa
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.186 mycelium
-
 Neurospora crassa
-

Storage Stability

EC Number Storage Stability Organism
2.4.1.186 -20°C, amylolyzed M-glycogenin preparation, at least 1 month without appreciable loss of activity Neurospora crassa
2.4.1.186 4°C, amylolyzed M-glycogenin preparation, at least 1 week without appreciable loss of activity Neurospora crassa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.186 UDP-glucose + glycogenin autoglycosylation reaction Escherichia coli UDP + glucosylated glycogenin forms glucosyl-alpha1,4-glucosyl linkage ?
2.4.1.186 UDP-glucose + glycogenin autoglycosylation reaction Neurospora crassa UDP + glucosylated glycogenin forms glucosyl-alpha1,4-glucosyl linkage ?
2.4.1.186 UDP-glucose + glycogenin enzyme forms the protein part of proteoglycogen Escherichia coli UDP + glucosylated glycogenin
-
 ?
2.4.1.186 UDP-glucose + glycogenin enzyme forms the protein part of proteoglycogen Neurospora crassa UDP + glucosylated glycogenin
-
 ?
2.4.1.186 UDP-glucose + glycogenin enzyme forms the protein part of proteoglycogen Escherichia coli UDP + glucosylated glycogenin forms glucosyl-alpha1,4-glucosyl linkage ?
2.4.1.186 UDP-glucose + glycogenin enzyme forms the protein part of proteoglycogen Neurospora crassa UDP + glucosylated glycogenin forms glucosyl-alpha1,4-glucosyl linkage ?
2.4.1.186 UDP-glucose + glycogenin UDP-glucose can not be replaced by ADP- or GDP-glucose Neurospora crassa UDP + glucosylated glycogenin forms glucosyl-alpha1,4-glucosyl linkage ?
2.4.1.186 UDP-glucose + n-dodecyl-beta-D-maltoside transglucosylation reaction Escherichia coli UDP + n-dodecyl-beta-D-maltotriose
-
 ?
2.4.1.186 UDP-glucose + n-dodecyl-beta-D-maltoside transglucosylation reaction Neurospora crassa UDP + n-dodecyl-beta-D-maltotriose
-
 ?
2.4.1.186 UDP-glucose + n-dodecyl-beta-D-maltoside simultaneously and independently of the autoglycosylation reaction Escherichia coli UDP + n-dodecyl-beta-D-maltotriose
-
 ?
2.4.1.186 UDP-glucose + n-dodecyl-beta-D-maltoside simultaneously and independently of the autoglycosylation reaction Neurospora crassa UDP + n-dodecyl-beta-D-maltotriose
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.186 ? x * 31000, SDS-PAGE Neurospora crassa
2.4.1.186 More enzyme forms the protein part of proteoglycogen Escherichia coli
2.4.1.186 More enzyme forms the protein part of proteoglycogen Neurospora crassa

Synonyms

EC Number Synonyms Comment Organism
2.4.1.186 M-glycogenin
-
 Escherichia coli
2.4.1.186 M-glycogenin
-
 Neurospora crassa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.186 30
-
 assay at Neurospora crassa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.186 7
-
 assay at Neurospora crassa