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Literature summary extracted from

  • Togayachi, A.; Akashima, T.; Ookubo, R.; Kudo, T.; Nishihara, S.; Iwasaki, H.; Natsume, A.; Mio, H.; Inokuchi, J.i.; Irimura, T.; Sasaki, K.; Narimatsu, H.
    Molecular cloning and characterization of UDP-GlcNAc:lactosylceramide beta1,3-N-acetylglucosaminyltransferase (beta3Gn-T5), an essential enzyme for the expression of HNK-1 and lewis X epitopes on glycolipids (2001), J. Biol. Chem., 276, 22032-22040.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.206 retinoic acid up-regulation of enzyme expression Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.206
-
 Rattus norvegicus
2.4.1.206 DNA sequence determination and analysis Homo sapiens
2.4.1.206 expression in Nawalma cells, membrane-bound Homo sapiens
2.4.1.206 partial sequence, not a full length clone, DNA sequence determination Rattus norvegicus
2.4.1.206 soluble expression as FLAG-tagged protein in Spodoptera frugiperda Sf21 cells via baculovirus infection Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.206 12-O-tetradecanoylphorbol-13-acetate down-regulation of enzyme expression Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.206 additional information Rattus norvegicus essential enzyme for the expression of HNK-1 and Lewis X epitopes on glycolipids ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.206 Homo sapiens Q9BYG0
-
-
2.4.1.206 Rattus norvegicus
-
-
-
2.4.1.206 Rattus norvegicus Q99NB2 not a full length clone
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.206 recombinant FLAG-tagged fusion protein from Sf21 insect cells Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.206 brain
-
 Rattus norvegicus
-
2.4.1.206 brain low content Homo sapiens
-
2.4.1.206 cerebellum
-
 Homo sapiens
-
2.4.1.206 cerebellum
-
 Rattus norvegicus
-
2.4.1.206 cerebral cortex fetal Rattus norvegicus
-
2.4.1.206 cerebral cortex not adult Rattus norvegicus
-
2.4.1.206 colon
-
 Homo sapiens
-
2.4.1.206 colonic adenocarcinoma cell
-
 Homo sapiens
-
2.4.1.206 hypophysis
-
 Homo sapiens
-
2.4.1.206 liver very low content Homo sapiens
-
2.4.1.206 lung
-
 Homo sapiens
-
2.4.1.206 lymph node low content Homo sapiens
-
2.4.1.206 additional information transcript content and activity in diverse tumour cell lines, overview, nearly undetectable content in leukemic cell lines Homo sapiens
-
2.4.1.206 additional information enzyme levels in brain regions during pre- and postnatal brain development Rattus norvegicus
-
2.4.1.206 placenta
-
 Homo sapiens
-
2.4.1.206 spleen low content Homo sapiens
-
2.4.1.206 testis
-
 Homo sapiens
-
2.4.1.206 thymus low content Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.206 additional information
-
 activity in diverse tumour cell lines, overview Homo sapiens
2.4.1.206 additional information
-
 activity during brain development Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.206 additional information essential enzyme for the expression of HNK-1 and Lewis X epitopes on glycolipids Rattus norvegicus ?
-
 ?
2.4.1.206 UDP-N-acetyl-D-glucosamine + D-galactosyl-1,4-beta-D-glucosylceramide
-
 Rattus norvegicus UDP + N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide
-
 ?
2.4.1.206 UDP-N-acetyl-D-glucosamine + D-galactosyl-1,4-beta-D-glucosylceramide i.e. lactosylceramide Homo sapiens UDP + N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide
-
 ?
2.4.1.206 UDP-N-acetyl-D-glucosamine + D-galactosyl-1,4-beta-D-glucosylceramide acceptor substrate is fluorescently labeled with 2-aminobenzamid or 2-pyridylamine Homo sapiens UDP + N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide
-
 ?
2.4.1.206 UDP-N-acetyl-D-glucosamine + D-galactosyl-1,4-beta-N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide acceptor substrate is fluorescently labeled with 2-aminobenzamid or 2-pyridylamine Homo sapiens UDP + N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-beta-N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.206 beta3Gn-T5
-
 Homo sapiens
2.4.1.206 beta3Gn-T5
-
 Rattus norvegicus
2.4.1.206 lactotriaosylceramide synthase
-
 Homo sapiens
2.4.1.206 lactotriaosylceramide synthase
-
 Rattus norvegicus
2.4.1.206 UDP-GlcNAc:lactosylceramide beta1,3-N-acetylglucosaminyltransferase
-
 Homo sapiens
2.4.1.206 UDP-GlcNAc:lactosylceramide beta1,3-N-acetylglucosaminyltransferase
-
 Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.206 37
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.206 7.2
-
 assay at Homo sapiens