Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Ikeda, Y.; Taniguchi, N.
    Enzymatic properties and biological functions of beta1,4-N-acetylglucosaminyltransferase III (2001), Trends Glycosci. Glycotechnol., 13, 167-176.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.144
-
 Rattus norvegicus
2.4.1.144 construction of transgenic mice, disruption of certain functions of apolipoprotein B leading to generation of a aftty liver Rattus norvegicus
2.4.1.144 expression in erythroleukemia cell line K562, leading to increased resistance to lysis by natural killer cells and enhanced spleen colonization ability Homo sapiens
2.4.1.144 expression of soluble His-tagged protein in Spodoptera frugiperda Sf 21 insect cells via baculovirus infection, secretion into the medium Rattus norvegicus
2.4.1.144 expression of wild-type and mutants in COS-1 cells Rattus norvegicus
2.4.1.144 overexpression in murine lung melanoma cell line B16-hm, leading to suppression of the formation of beta-1,6-tri- and tetraantennary N-linked oligosaccharides by inhibiting the responsible enzymes, e.g. N-acetylglucosamine transferase V, EC 2.4.1.155, decrease of the metastatic potential of B16-hm melanoma in vivo, changed phenotype Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.144 N243Q
-
 Rattus norvegicus
2.4.1.144 N243Q/N261Q
-
 Rattus norvegicus
2.4.1.144 N243Q/N261Q/N399Q
-
 Rattus norvegicus
2.4.1.144 N243Q/N399Q
-
 Rattus norvegicus
2.4.1.144 N261Q
-
 Rattus norvegicus
2.4.1.144 N261Q/N399Q
-
 Rattus norvegicus
2.4.1.144 N399Q
-
 Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.144 0.42
-
 UDP-N-acetyl-D-glucosamine recombinant enzyme Rattus norvegicus
2.4.1.144 1
-
 UDP-D-glucose recombinant enzyme Rattus norvegicus
2.4.1.144 3.6
-
 UDP-N-acetyl-D-galactosamine recombinant enzyme Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.144 Golgi apparatus
-
 Homo sapiens 5794
-
2.4.1.144 Golgi apparatus deglycosylated mutants are not localized in the Golgi apparatus Rattus norvegicus 5794
-
2.4.1.144 membrane
-
 Gallus gallus 16020
-
2.4.1.144 membrane
-
 Homo sapiens 16020
-
2.4.1.144 membrane
-
 Rattus norvegicus 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.144 Mn2+
-
 Gallus gallus
2.4.1.144 Mn2+
-
 Homo sapiens
2.4.1.144 Mn2+
-
 Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.144 additional information
-
 amino acid and DNA sequence Rattus norvegicus
2.4.1.144 additional information
-
 type II transmembrane protein Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.144 additional information Homo sapiens biosynthetic pathway of the core structures of Asn-linked sugar chains, overview ?
-
 ?
2.4.1.144 additional information Rattus norvegicus biosynthetic pathway of the core structures of Asn-linked sugar chains, overview ?
-
 ?
2.4.1.144 UDP-N-acetyl-D-glucosamine + (N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6)-beta-D-mannosyl-1,4-N-acetyl-beta-D-glucosaminyl-R Homo sapiens key regulatory role in N-glycan biosynthesis, responsible for cancer-associated alterations UDP + N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6)-(N-acetyl-beta-D-glucosaminyl-1,4)-beta-D-mannosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
-
 ?
2.4.1.144 UDP-N-acetyl-D-glucosamine + (N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6)-beta-D-mannosyl-1,4-N-acetyl-beta-D-glucosaminyl-R Rattus norvegicus key regulatory role in N-glycan biosynthesis, responsible for cancer-associated alterations UDP + N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6)-(N-acetyl-beta-D-glucosaminyl-1,4)-beta-D-mannosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
-
 ?
2.4.1.144 UDP-N-acetyl-D-glucosamine + (N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6)-beta-D-mannosyl-1,4-N-acetyl-beta-D-glucosaminyl-R Homo sapiens important role in carcinogenesis and cancer metastasis UDP + N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6)-(N-acetyl-beta-D-glucosaminyl-1,4)-beta-D-mannosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
-
 ?
2.4.1.144 UDP-N-acetyl-D-glucosamine + (N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6)-beta-D-mannosyl-1,4-N-acetyl-beta-D-glucosaminyl-R Rattus norvegicus important role in carcinogenesis and cancer metastasis UDP + N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6)-(N-acetyl-beta-D-glucosaminyl-1,4)-beta-D-mannosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
-
 ?
2.4.1.144 UDP-N-acetyl-D-glucosamine + (N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6)-beta-D-mannosyl-1,4-N-acetyl-beta-D-glucosaminyl-R Homo sapiens key enzyme in the biosynthesis of N-glycans since it inhibits the other involved glycosyltransferases by adding the GlcNAc-residue in an beta-1,4- linkage UDP + N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6)-(N-acetyl-beta-D-glucosaminyl-1,4)-beta-D-mannosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
-
 ?
2.4.1.144 UDP-N-acetyl-D-glucosamine + (N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6)-beta-D-mannosyl-1,4-N-acetyl-beta-D-glucosaminyl-R Rattus norvegicus key enzyme in the biosynthesis of N-glycans since it inhibits the other involved glycosyltransferases by adding the GlcNAc-residue in an beta-1,4- linkage UDP + N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6)-(N-acetyl-beta-D-glucosaminyl-1,4)-beta-D-mannosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
-
 ?
2.4.1.144 UDP-N-acetyl-D-glucosamine + (N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6)-beta-D-mannosyl-1,4-N-acetyl-beta-D-glucosaminyl-R Gallus gallus R represents the remainder of the N-oligosaccharide core (+/-fucose), essential for biosynthesis of complex N-linked oligosaccharides UDP + N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6)-(N-acetyl-beta-D-glucosaminyl-1,4)-beta-D-mannosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
-
 ?
2.4.1.144 UDP-N-acetyl-D-glucosamine + (N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6)-beta-D-mannosyl-1,4-N-acetyl-beta-D-glucosaminyl-R Homo sapiens R represents the remainder of the N-oligosaccharide core (+/-fucose), essential for biosynthesis of complex N-linked oligosaccharides UDP + N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6)-(N-acetyl-beta-D-glucosaminyl-1,4)-beta-D-mannosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
-
 ?
2.4.1.144 UDP-N-acetyl-D-glucosamine + (N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6)-beta-D-mannosyl-1,4-N-acetyl-beta-D-glucosaminyl-R Rattus norvegicus R represents the remainder of the N-oligosaccharide core (+/-fucose), essential for biosynthesis of complex N-linked oligosaccharides UDP + N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6)-(N-acetyl-beta-D-glucosaminyl-1,4)-beta-D-mannosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
-
 ?
2.4.1.144 UDP-N-acetyl-D-glucosamine + (N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6)-beta-D-mannosyl-1,4-N-acetyl-beta-D-glucosaminyl-R Homo sapiens numerous target proteins, multifacial glycosyltransferase UDP + N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6)-(N-acetyl-beta-D-glucosaminyl-1,4)-beta-D-mannosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
-
 ?
2.4.1.144 UDP-N-acetyl-D-glucosamine + (N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6)-beta-D-mannosyl-1,4-N-acetyl-beta-D-glucosaminyl-R Rattus norvegicus numerous target proteins, multifacial glycosyltransferase UDP + N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6)-(N-acetyl-beta-D-glucosaminyl-1,4)-beta-D-mannosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
-
 ?
2.4.1.144 UDP-N-acetyl-D-glucosamine + E-cadherin Homo sapiens recombinant enzyme expressed in murine lung melanoma cells UDP + E-cadherin with bisecting N-acetyl-D-glucosamine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.144 Gallus gallus
-
-
-
2.4.1.144 Homo sapiens
-
 single copy gene, no isoforms
-
2.4.1.144 Rattus norvegicus
-
-
-
2.4.1.144 Rattus norvegicus Q02527
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.4.1.144 glycoprotein 3 N-glycosylation sites: Asn243, Asn261, Asn399, essential for activity, tunicamycin-treated enzyme, which is not glycosylated, shows no activity Rattus norvegicus

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.144
-
 Rattus norvegicus
2.4.1.144 primary structure Rattus norvegicus

Reaction

EC Number Reaction Comment Organism Reaction ID
2.4.1.144 UDP-N-acetyl-alpha-D-glucosamine + beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-N-Asn-[protein] = UDP + beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->6)]-[beta-D-GlcNAc-(1->4)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-N-Asn-[protein] sequential, random or partially random mechanism Rattus norvegicus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.144 brain
-
 Homo sapiens
-
2.4.1.144 brain
-
 Rattus norvegicus
-
2.4.1.144 kidney
-
 Homo sapiens
-
2.4.1.144 kidney
-
 Rattus norvegicus
-
2.4.1.144 liver
-
 Gallus gallus
-
2.4.1.144 liver very low amount Homo sapiens
-
2.4.1.144 liver very low amount Rattus norvegicus
-
2.4.1.144 additional information
-
 Rattus norvegicus
-
2.4.1.144 additional information multiple promotors allow tissue-specific expression Homo sapiens
-
2.4.1.144 oviduct
-
 Gallus gallus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.144 additional information UDP-galactose is no substrate Rattus norvegicus ?
-
 ?
2.4.1.144 additional information specificity towards the sugar moiety of the donor substrate appears to be critically determined during the catalytic process but not during binding to the enzyme in ground state Rattus norvegicus ?
-
 ?
2.4.1.144 additional information biosynthetic pathway of the core structures of Asn-linked sugar chains, overview Homo sapiens ?
-
 ?
2.4.1.144 additional information biosynthetic pathway of the core structures of Asn-linked sugar chains, overview Rattus norvegicus ?
-
 ?
2.4.1.144 UDP-D-glucose + (N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6)-beta-D-mannosyl-1,4-N-acetyl-beta-D-glucosaminyl-R 0.2% activity compared to donor substrate UDP-N-acetyl-D-glucosamine Rattus norvegicus UDP + N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6)-(beta-D-glucosyl-1,4)-beta-D-mannosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
-
 ?
2.4.1.144 UDP-N-acetyl-D-galactosamine + (N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6)-beta-D-mannosyl-1,4-N-acetyl-beta-D-glucosaminyl-R 0.1% activity compared to donor substrate UDP-N-acetyl-D-glucosamine Rattus norvegicus UDP + N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6)-(N-acetyl-beta-D-galactosaminyl-1,4)-beta-D-mannosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
-
 ?
2.4.1.144 UDP-N-acetyl-D-glucosamine + (N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6)-beta-D-mannosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
-
 Rattus norvegicus UDP + N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6)-(N-acetyl-beta-D-glucosaminyl-1,4)-beta-D-mannosyl-1,4-N-acetyl-beta-D-glucosaminyl-R incorporates a N-acetylglucosamine residue in beta-1,4-linkage to beta-linked mannosyl, i.e. bisecting N-acetylglucosamine ir
2.4.1.144 UDP-N-acetyl-D-glucosamine + (N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6)-beta-D-mannosyl-1,4-N-acetyl-beta-D-glucosaminyl-R substrate specificity Gallus gallus UDP + N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6)-(N-acetyl-beta-D-glucosaminyl-1,4)-beta-D-mannosyl-1,4-N-acetyl-beta-D-glucosaminyl-R incorporates a N-acetylglucosamine residue in beta-1,4-linkage to beta-linked mannosyl, i.e. bisecting N-acetylglucosamine ir
2.4.1.144 UDP-N-acetyl-D-glucosamine + (N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6)-beta-D-mannosyl-1,4-N-acetyl-beta-D-glucosaminyl-R substrate specificity Homo sapiens UDP + N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6)-(N-acetyl-beta-D-glucosaminyl-1,4)-beta-D-mannosyl-1,4-N-acetyl-beta-D-glucosaminyl-R incorporates a N-acetylglucosamine residue in beta-1,4-linkage to beta-linked mannosyl, i.e. bisecting N-acetylglucosamine ir
2.4.1.144 UDP-N-acetyl-D-glucosamine + (N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6)-beta-D-mannosyl-1,4-N-acetyl-beta-D-glucosaminyl-R substrate specificity Rattus norvegicus UDP + N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6)-(N-acetyl-beta-D-glucosaminyl-1,4)-beta-D-mannosyl-1,4-N-acetyl-beta-D-glucosaminyl-R incorporates a N-acetylglucosamine residue in beta-1,4-linkage to beta-linked mannosyl, i.e. bisecting N-acetylglucosamine ir
2.4.1.144 UDP-N-acetyl-D-glucosamine + (N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6)-beta-D-mannosyl-1,4-N-acetyl-beta-D-glucosaminyl-R structural requirements, stereochemistry Homo sapiens UDP + N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6)-(N-acetyl-beta-D-glucosaminyl-1,4)-beta-D-mannosyl-1,4-N-acetyl-beta-D-glucosaminyl-R incorporates a N-acetylglucosamine residue in beta-1,4-linkage to beta-linked mannosyl, i.e. bisecting N-acetylglucosamine ir
2.4.1.144 UDP-N-acetyl-D-glucosamine + (N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6)-beta-D-mannosyl-1,4-N-acetyl-beta-D-glucosaminyl-R structural requirements, stereochemistry Rattus norvegicus UDP + N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6)-(N-acetyl-beta-D-glucosaminyl-1,4)-beta-D-mannosyl-1,4-N-acetyl-beta-D-glucosaminyl-R incorporates a N-acetylglucosamine residue in beta-1,4-linkage to beta-linked mannosyl, i.e. bisecting N-acetylglucosamine ir
2.4.1.144 UDP-N-acetyl-D-glucosamine + (N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6)-beta-D-mannosyl-1,4-N-acetyl-beta-D-glucosaminyl-R beta1,2-GlcNAc linked to alpha1,3-Man is required for activity, beta1,2-GlcNAc linked to alpha1,6-Man is not required Gallus gallus UDP + N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6)-(N-acetyl-beta-D-glucosaminyl-1,4)-beta-D-mannosyl-1,4-N-acetyl-beta-D-glucosaminyl-R incorporates a N-acetylglucosamine residue in beta-1,4-linkage to beta-linked mannosyl, i.e. bisecting N-acetylglucosamine ir
2.4.1.144 UDP-N-acetyl-D-glucosamine + (N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6)-beta-D-mannosyl-1,4-N-acetyl-beta-D-glucosaminyl-R beta1,2-GlcNAc linked to alpha1,3-Man is required for activity, beta1,2-GlcNAc linked to alpha1,6-Man is not required Homo sapiens UDP + N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6)-(N-acetyl-beta-D-glucosaminyl-1,4)-beta-D-mannosyl-1,4-N-acetyl-beta-D-glucosaminyl-R incorporates a N-acetylglucosamine residue in beta-1,4-linkage to beta-linked mannosyl, i.e. bisecting N-acetylglucosamine ir
2.4.1.144 UDP-N-acetyl-D-glucosamine + (N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6)-beta-D-mannosyl-1,4-N-acetyl-beta-D-glucosaminyl-R beta1,2-GlcNAc linked to alpha1,3-Man is required for activity, beta1,2-GlcNAc linked to alpha1,6-Man is not required Rattus norvegicus UDP + N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6)-(N-acetyl-beta-D-glucosaminyl-1,4)-beta-D-mannosyl-1,4-N-acetyl-beta-D-glucosaminyl-R incorporates a N-acetylglucosamine residue in beta-1,4-linkage to beta-linked mannosyl, i.e. bisecting N-acetylglucosamine ir
2.4.1.144 UDP-N-acetyl-D-glucosamine + (N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6)-beta-D-mannosyl-1,4-N-acetyl-beta-D-glucosaminyl-R any agalacto form of the bi-, tri- and tetraantennary sugar chains is capable of serving as ana cceptor substrate Gallus gallus UDP + N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6)-(N-acetyl-beta-D-glucosaminyl-1,4)-beta-D-mannosyl-1,4-N-acetyl-beta-D-glucosaminyl-R incorporates a N-acetylglucosamine residue in beta-1,4-linkage to beta-linked mannosyl, i.e. bisecting N-acetylglucosamine ir
2.4.1.144 UDP-N-acetyl-D-glucosamine + (N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6)-beta-D-mannosyl-1,4-N-acetyl-beta-D-glucosaminyl-R any agalacto form of the bi-, tri- and tetraantennary sugar chains is capable of serving as ana cceptor substrate Homo sapiens UDP + N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6)-(N-acetyl-beta-D-glucosaminyl-1,4)-beta-D-mannosyl-1,4-N-acetyl-beta-D-glucosaminyl-R incorporates a N-acetylglucosamine residue in beta-1,4-linkage to beta-linked mannosyl, i.e. bisecting N-acetylglucosamine ir
2.4.1.144 UDP-N-acetyl-D-glucosamine + (N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6)-beta-D-mannosyl-1,4-N-acetyl-beta-D-glucosaminyl-R any agalacto form of the bi-, tri- and tetraantennary sugar chains is capable of serving as ana cceptor substrate Rattus norvegicus UDP + N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6)-(N-acetyl-beta-D-glucosaminyl-1,4)-beta-D-mannosyl-1,4-N-acetyl-beta-D-glucosaminyl-R incorporates a N-acetylglucosamine residue in beta-1,4-linkage to beta-linked mannosyl, i.e. bisecting N-acetylglucosamine ir
2.4.1.144 UDP-N-acetyl-D-glucosamine + (N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6)-beta-D-mannosyl-1,4-N-acetyl-beta-D-glucosaminyl-R beta1,4-galactosylation of N-acetylglucosaminyl-1,2-alpha-mannosyl-1,3-beta-mannosyl moiety prevents transferase III action Homo sapiens UDP + N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6)-(N-acetyl-beta-D-glucosaminyl-1,4)-beta-D-mannosyl-1,4-N-acetyl-beta-D-glucosaminyl-R incorporates a N-acetylglucosamine residue in beta-1,4-linkage to beta-linked mannosyl, i.e. bisecting N-acetylglucosamine ir
2.4.1.144 UDP-N-acetyl-D-glucosamine + (N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6)-beta-D-mannosyl-1,4-N-acetyl-beta-D-glucosaminyl-R beta1,4-galactosylation of N-acetylglucosaminyl-1,2-alpha-mannosyl-1,3-beta-mannosyl moiety prevents transferase III action Rattus norvegicus UDP + N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6)-(N-acetyl-beta-D-glucosaminyl-1,4)-beta-D-mannosyl-1,4-N-acetyl-beta-D-glucosaminyl-R incorporates a N-acetylglucosamine residue in beta-1,4-linkage to beta-linked mannosyl, i.e. bisecting N-acetylglucosamine ir
2.4.1.144 UDP-N-acetyl-D-glucosamine + (N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6)-beta-D-mannosyl-1,4-N-acetyl-beta-D-glucosaminyl-R R represents the remainder of the N-oligosaccharide core, (+/-)-fucose, favourite substrate Homo sapiens UDP + N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6)-(N-acetyl-beta-D-glucosaminyl-1,4)-beta-D-mannosyl-1,4-N-acetyl-beta-D-glucosaminyl-R incorporates a N-acetylglucosamine residue in beta-1,4-linkage to beta-linked mannosyl, i.e. bisecting N-acetylglucosamine ir
2.4.1.144 UDP-N-acetyl-D-glucosamine + (N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6)-beta-D-mannosyl-1,4-N-acetyl-beta-D-glucosaminyl-R R represents the remainder of the N-oligosaccharide core, (+/-)-fucose, favourite substrate Rattus norvegicus UDP + N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6)-(N-acetyl-beta-D-glucosaminyl-1,4)-beta-D-mannosyl-1,4-N-acetyl-beta-D-glucosaminyl-R incorporates a N-acetylglucosamine residue in beta-1,4-linkage to beta-linked mannosyl, i.e. bisecting N-acetylglucosamine ir
2.4.1.144 UDP-N-acetyl-D-glucosamine + (N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6)-beta-D-mannosyl-1,4-N-acetyl-beta-D-glucosaminyl-R key regulatory role in N-glycan biosynthesis, responsible for cancer-associated alterations Homo sapiens UDP + N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6)-(N-acetyl-beta-D-glucosaminyl-1,4)-beta-D-mannosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
-
 ?
2.4.1.144 UDP-N-acetyl-D-glucosamine + (N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6)-beta-D-mannosyl-1,4-N-acetyl-beta-D-glucosaminyl-R key regulatory role in N-glycan biosynthesis, responsible for cancer-associated alterations Rattus norvegicus UDP + N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6)-(N-acetyl-beta-D-glucosaminyl-1,4)-beta-D-mannosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
-
 ?
2.4.1.144 UDP-N-acetyl-D-glucosamine + (N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6)-beta-D-mannosyl-1,4-N-acetyl-beta-D-glucosaminyl-R important role in carcinogenesis and cancer metastasis Homo sapiens UDP + N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6)-(N-acetyl-beta-D-glucosaminyl-1,4)-beta-D-mannosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
-
 ?
2.4.1.144 UDP-N-acetyl-D-glucosamine + (N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6)-beta-D-mannosyl-1,4-N-acetyl-beta-D-glucosaminyl-R important role in carcinogenesis and cancer metastasis Rattus norvegicus UDP + N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6)-(N-acetyl-beta-D-glucosaminyl-1,4)-beta-D-mannosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
-
 ?
2.4.1.144 UDP-N-acetyl-D-glucosamine + (N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6)-beta-D-mannosyl-1,4-N-acetyl-beta-D-glucosaminyl-R key enzyme in the biosynthesis of N-glycans since it inhibits the other involved glycosyltransferases by adding the GlcNAc-residue in an beta-1,4- linkage Homo sapiens UDP + N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6)-(N-acetyl-beta-D-glucosaminyl-1,4)-beta-D-mannosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
-
 ?
2.4.1.144 UDP-N-acetyl-D-glucosamine + (N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6)-beta-D-mannosyl-1,4-N-acetyl-beta-D-glucosaminyl-R key enzyme in the biosynthesis of N-glycans since it inhibits the other involved glycosyltransferases by adding the GlcNAc-residue in an beta-1,4- linkage Rattus norvegicus UDP + N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6)-(N-acetyl-beta-D-glucosaminyl-1,4)-beta-D-mannosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
-
 ?
2.4.1.144 UDP-N-acetyl-D-glucosamine + (N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6)-beta-D-mannosyl-1,4-N-acetyl-beta-D-glucosaminyl-R R represents the remainder of the N-oligosaccharide core (+/-fucose), essential for biosynthesis of complex N-linked oligosaccharides Gallus gallus UDP + N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6)-(N-acetyl-beta-D-glucosaminyl-1,4)-beta-D-mannosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
-
 ?
2.4.1.144 UDP-N-acetyl-D-glucosamine + (N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6)-beta-D-mannosyl-1,4-N-acetyl-beta-D-glucosaminyl-R R represents the remainder of the N-oligosaccharide core (+/-fucose), essential for biosynthesis of complex N-linked oligosaccharides Homo sapiens UDP + N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6)-(N-acetyl-beta-D-glucosaminyl-1,4)-beta-D-mannosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
-
 ?
2.4.1.144 UDP-N-acetyl-D-glucosamine + (N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6)-beta-D-mannosyl-1,4-N-acetyl-beta-D-glucosaminyl-R R represents the remainder of the N-oligosaccharide core (+/-fucose), essential for biosynthesis of complex N-linked oligosaccharides Rattus norvegicus UDP + N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6)-(N-acetyl-beta-D-glucosaminyl-1,4)-beta-D-mannosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
-
 ?
2.4.1.144 UDP-N-acetyl-D-glucosamine + (N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6)-beta-D-mannosyl-1,4-N-acetyl-beta-D-glucosaminyl-R numerous target proteins, multifacial glycosyltransferase Homo sapiens UDP + N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6)-(N-acetyl-beta-D-glucosaminyl-1,4)-beta-D-mannosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
-
 ?
2.4.1.144 UDP-N-acetyl-D-glucosamine + (N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6)-beta-D-mannosyl-1,4-N-acetyl-beta-D-glucosaminyl-R numerous target proteins, multifacial glycosyltransferase Rattus norvegicus UDP + N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6)-(N-acetyl-beta-D-glucosaminyl-1,4)-beta-D-mannosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
-
 ?
2.4.1.144 UDP-N-acetyl-D-glucosamine + E-cadherin recombinant enzyme expressed in murine lung melanoma cells Homo sapiens UDP + E-cadherin with bisecting N-acetyl-D-glucosamine
-
 ?
2.4.1.144 UDP-N-acetyl-D-glucosamine + transferrin
-
 Rattus norvegicus UDP + transferrin with bisecting N-acetyl-D-glucosamine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.144 beta1,4-N-acetylglucosaminyltransferase III
-
 Gallus gallus
2.4.1.144 beta1,4-N-acetylglucosaminyltransferase III
-
 Homo sapiens
2.4.1.144 beta1,4-N-acetylglucosaminyltransferase III
-
 Rattus norvegicus
2.4.1.144 GnT-III
-
 Gallus gallus
2.4.1.144 GnT-III
-
 Homo sapiens
2.4.1.144 GnT-III
-
 Rattus norvegicus