EC Number | Cloned (Comment) | Organism |
---|---|---|
2.5.1.19 | overexpression of wild-type, His-tagged wild-type and several mutant enzymes in Escherichia coli | Escherichia coli |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.5.1.19 | D313A | 0.42% of wild-type activity | Escherichia coli |
2.5.1.19 | D313N | 5% of wild-type activity | Escherichia coli |
2.5.1.19 | D49A | 41% of wild-type activity | Escherichia coli |
2.5.1.19 | E341A | 0.3% of wild-type activity | Escherichia coli |
2.5.1.19 | E341Q | 10% of wild-type activity | Escherichia coli |
2.5.1.19 | H385A | 0.08% of wild-type activity | Escherichia coli |
2.5.1.19 | K22A | 0.7% of wild-type activity | Escherichia coli |
2.5.1.19 | K22R | 3% of wild-type activity | Escherichia coli |
2.5.1.19 | K340A | 2.4% of wild-type activity | Escherichia coli |
2.5.1.19 | K411A | 10.4% of wild-type activity | Escherichia coli |
2.5.1.19 | N94A | 50% of wild-type activity | Escherichia coli |
2.5.1.19 | Q171A | 1.7% of wild-type activity | Escherichia coli |
2.5.1.19 | R100M | 0.2% of wild-type activity | Escherichia coli |
2.5.1.19 | R124A | 19.6% of wild-type activity | Escherichia coli |
2.5.1.19 | R344K | 31.7% of wild-type activity | Escherichia coli |
2.5.1.19 | R344M | 16.3% of wild-type activity | Escherichia coli |
2.5.1.19 | R386M | 15.8% of wild-type activity | Escherichia coli |
2.5.1.19 | Y200F | 1% of wild-type activity | Escherichia coli |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.5.1.19 | Escherichia coli | P0A6D3 | - |
- |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.5.1.19 | 20 | - |
recombinant His-tagged wild-type enzyme | Escherichia coli |
2.5.1.19 | 24 | - |
recombinant wild-type enzyme | Escherichia coli |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.5.1.19 | phosphoenolpyruvate + 3-phosphoshikimate | - |
Escherichia coli | phosphate + 5-enolpyruvylshikimate 3-phosphate | - |
r |