Literature summary extracted from

Eschenburg, S.; Healy, M.L.; Priestman, M.A.; Lushington, G.H.; Schonbrunn, E.
How the mutation glycine96 to alanine confers glyphosate insensitivity to 5-enolpyruvyl shikimate-3-phosphate synthase from Escherichia coli (2002), Planta, 216, 129-135.

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
2.5.1.19	expression of wild-type and G96A mutant enzyme in Escherichia coli	Escherichia coli

Protein Variants

EC Number	Protein Variants	Comment	Organism
2.5.1.19	G96A	glyphosate-insensitive	Escherichia coli
2.5.1.19	G96A	glyphosate-insensitive	Klebsiella pneumoniae

Inhibitors

EC Number	Inhibitors	Comment	Organism	Structure
2.5.1.19	N-phosphonomethylglycine	-	Escherichia coli

KM Value [mM]

EC Number	KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
2.5.1.19	0.088	-	phosphoenolpyruvate	wild-type enzyme	Escherichia coli
2.5.1.19	0.12	-	shikimate 3-phosphate	wild-type and G96A mutant enzyme	Escherichia coli
2.5.1.19	2.8	-	phosphoenolpyruvate	G96A mutant enzyme	Escherichia coli

Organism

EC Number	Organism	UniProt	Comment	Textmining
2.5.1.19	Escherichia coli	-	-	-
2.5.1.19	Klebsiella pneumoniae	-	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
2.5.1.19	recombinant wild-type and G96A mutant enzyme	Escherichia coli

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2.5.1.19	phosphoenolpyruvate + 3-phosphoshikimate	-	Escherichia coli	phosphate + 5-enolpyruvylshikimate 3-phosphate	-	r
2.5.1.19	phosphoenolpyruvate + 3-phosphoshikimate	-	Klebsiella pneumoniae	phosphate + 5-enolpyruvylshikimate 3-phosphate	-	r

Ki Value [mM]

EC Number	Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
2.5.1.19	0.01	-	N-phosphonomethylglycine	-	Escherichia coli

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Release 2023.1 (January 2023)