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Literature summary extracted from

  • Du, W.; Wallis, N.G.; Mazzulla, M.J.; Chalker, A.F.; Zhang, L.; Liu, W.S.; Kallender, H.; Payne, D.J.
    Characterization of Streptococcus pneumoniae 5-enolpyruvylshikimate 3-phosphate synthase and its activation by univalent cations (2000), Eur. J. Biochem., 267, 222-227.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.5.1.19 NH4Cl strong activation of forward reaction, effectiveness of activation in descending order: NH4Cl, Rb+, K+ Streptococcus pneumoniae

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.19 expression in Escherichia coli Streptococcus pneumoniae

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.19 N-phosphonomethylglycine
-
 Streptococcus pneumoniae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.19 0.00195
-
 5-enolpyruvylshikimate 3-phosphate reverse reaction, in the presence of 50 mM KCl Streptococcus pneumoniae
2.5.1.19 0.022
-
 phosphoenolpyruvate in the presence of 100 mM NH4Cl Streptococcus pneumoniae
2.5.1.19 0.023
-
 phosphoenolpyruvate in the presence of 1 mM NH4Cl and 100 mM KCl Streptococcus pneumoniae
2.5.1.19 0.031
-
 shikimate 3-phosphate in the presence of 100 mM NH4Cl Streptococcus pneumoniae
2.5.1.19 0.049
-
 shikimate 3-phosphate in the presence of 1 mM NH4Cl and 10 mM KCl Streptococcus pneumoniae
2.5.1.19 0.085
-
 phosphoenolpyruvate in the presence of 1 mM NH4Cl and 10 mM KCl Streptococcus pneumoniae
2.5.1.19 0.091
-
 phosphoenolpyruvate in the presence of 10 mM NH4Cl Streptococcus pneumoniae
2.5.1.19 0.1
-
 phosphoenolpyruvate in the presence of 1 mM NH4Cl Streptococcus pneumoniae
2.5.1.19 0.109
-
 shikimate 3-phosphate in the presence of 1 mM NH4Cl and 100 mM KCl Streptococcus pneumoniae
2.5.1.19 0.118
-
 shikimate 3-phosphate in the presence of 10 mM NH4Cl Streptococcus pneumoniae
2.5.1.19 0.145
-
 shikimate 3-phosphate in the presence of 1 mM NH4Cl Streptococcus pneumoniae
2.5.1.19 0.343
-
 phosphate reverse reaction, in the presence of 50 mM KCl Streptococcus pneumoniae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.19 K+ strong activation of forward reaction, effectiveness of activation in descending order: NH4Cl, Rb+, K+ Streptococcus pneumoniae
2.5.1.19 Rb+ strong activation of forward reaction, effectiveness of activation in descending order: NH4Cl, Rb+, K+ Streptococcus pneumoniae

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.19 Streptococcus pneumoniae Q9S400
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.19 recombinant enzyme, ammonium sulfate, hydrophobic interaction chromatography, anion-exchange chromatography Streptococcus pneumoniae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.19 phosphoenolpyruvate + 3-phosphoshikimate
-
 Streptococcus pneumoniae phosphate + 5-enolpyruvylshikimate 3-phosphate
-
 r

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5.1.19 0.22
-
 phosphate reverse reaction, in the presence of 50 mM KCl Streptococcus pneumoniae
2.5.1.19 0.23
-
 5-enolpyruvylshikimate 3-phosphate reverse reaction, in the presence of 50 mM KCl Streptococcus pneumoniae
2.5.1.19 2
-
 phosphoenolpyruvate in the presence of 1 mM NH4Cl and 10 mM KCl Streptococcus pneumoniae
2.5.1.19 2.1
-
 phosphoenolpyruvate in the presence of 1 mM NH4Cl Streptococcus pneumoniae
2.5.1.19 2.8
-
 phosphoenolpyruvate in the presence of 10 mM NH4Cl Streptococcus pneumoniae
2.5.1.19 3
-
 phosphoenolpyruvate in the presence of 100 mM NH4Cl Streptococcus pneumoniae
2.5.1.19 12.8
-
 phosphoenolpyruvate in the presence of 1 mM NH4Cl and 100 mM KCl Streptococcus pneumoniae

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.5.1.19 0.0003
-
 N-phosphonomethylglycine in the presence of 100 mM NH4Cl Streptococcus pneumoniae
2.5.1.19 0.005
-
 N-phosphonomethylglycine in the presence of 1 mM NH4Cl and 100 mM K+ Streptococcus pneumoniae
2.5.1.19 0.098
-
 N-phosphonomethylglycine in the presence of 1 mM NH4Cl and 10 mM K+ Streptococcus pneumoniae
2.5.1.19 0.185
-
 N-phosphonomethylglycine in the presence of 1 mM NH4Cl Streptococcus pneumoniae