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Literature summary extracted from

  • Shuttleworth, W.A.; Evans, J.N.S.
    The H385N mutant of 5-enolpyruvylshikimate-3-phosphate synthase: kinetics, fluorescence, and nuclear magnetic resonance studies (1996), Arch. Biochem. Biophys., 334, 37-42.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.19
-
 Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.19 N-phosphonomethylglycine
-
 Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.19 0.007
-
 5-enolpyruvylshikimate 3-phosphate H385N mutant enzyme Escherichia coli
2.5.1.19 0.011
-
 5-enolpyruvylshikimate 3-phosphate wild-type enzyme Escherichia coli
2.5.1.19 0.07
-
 shikimate 3-phosphate H385 mutant enzyme Escherichia coli
2.5.1.19 0.1
-
 phosphoenolpyruvate wild-type enzyme Escherichia coli
2.5.1.19 0.106
-
 phosphoenolpyruvate H385N mutant enzyme Escherichia coli
2.5.1.19 0.135
-
 shikimate 3-phosphate wild-type enzyme Escherichia coli
2.5.1.19 1.2
-
 phosphate H385N mutant enzyme Escherichia coli
2.5.1.19 4.6
-
 phosphate wild-type enzyme Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.19 Escherichia coli
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.19 10
-
-
 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.19 phosphoenolpyruvate + 3-phosphoshikimate
-
 Escherichia coli phosphate + 5-enolpyruvylshikimate 3-phosphate
-
 r

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.19 7
-
 H385N mutant enzyme Escherichia coli
2.5.1.19 7.8
-
 wild-type enzyme Escherichia coli

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.5.1.19 5.6 8.4 H385N mutantt enzyme Escherichia coli
2.5.1.19 6 8.4 wild-type enzyme Escherichia coli

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.5.1.19 0.0008
-
 N-phosphonomethylglycine H385N mutant enzyme Escherichia coli
2.5.1.19 0.0012
-
 N-phosphonomethylglycine wild-type enzyme Escherichia coli