EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
2.5.1.19 | NH4Cl | maximal activation at 100 mM | Bacillus subtilis |
EC Number | Cloned (Comment) | Organism |
---|---|---|
2.5.1.19 | expression of wild-type and R24D, P105S, H385L mutant enzymes in Escherichia coli | Bacillus subtilis |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.5.1.19 | H385L | 0.2% of wild-type activity, 2fold activation at 100 mM NH4Cl | Bacillus subtilis |
2.5.1.19 | H385N | 6% of wild-type activity | Bacillus subtilis |
2.5.1.19 | P105S | 69% of wild-type activity, 8fold activation at 100 mM NH4Cl | Bacillus subtilis |
2.5.1.19 | R24D | 0.8% of wild-type activity, 2fold activation at 100 mM NH4Cl | Bacillus subtilis |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.5.1.19 | N-phosphonomethylglycine | 0.25 mM, 50% inhibition of NH4Cl activated enzyme, 75 mM, 50% inhibition of nonactivated enzyme | Bacillus subtilis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.5.1.19 | 0.09 | - |
phosphoenolpyruvate | wild-type enzyme | Bacillus subtilis | |
2.5.1.19 | 0.16 | - |
shikimate 3-phosphate | H385 mutant enzyme | Bacillus subtilis | |
2.5.1.19 | 0.2 | - |
phosphoenolpyruvate | R24D mutant enzyme | Bacillus subtilis | |
2.5.1.19 | 0.2 | - |
shikimate 3-phosphate | R24D mutant enzyme | Bacillus subtilis | |
2.5.1.19 | 0.33 | - |
shikimate 3-phosphate | wild-type enzyme | Bacillus subtilis | |
2.5.1.19 | 0.43 | - |
shikimate 3-phosphate | P105S mutant enzyme | Bacillus subtilis | |
2.5.1.19 | 0.59 | - |
phosphoenolpyruvate | P105S mutant enzyme | Bacillus subtilis | |
2.5.1.19 | 1 | - |
phosphoenolpyruvate | H385L mutant enzyme | Bacillus subtilis |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.5.1.19 | 48000 | - |
mixture of monomers and dimers in the absence of NH4Cl, only monomers in the presence of NH4Cl, gel filtration | Bacillus subtilis |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.5.1.19 | phosphoenolpyruvate + 3-phosphoshikimate | Bacillus subtilis | involved in aromatic acid biosynthesis | phosphate + 5-enolpyruvylshikimate 3-phosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.5.1.19 | Bacillus subtilis | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.5.1.19 | recombinant enzyme, ammonium sulfate | Bacillus subtilis |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.5.1.19 | 10.4 | - |
- |
Bacillus subtilis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.5.1.19 | phosphoenolpyruvate + 3-phosphoshikimate | - |
Bacillus subtilis | phosphate + 5-enolpyruvylshikimate 3-phosphate | i.e. 5-O-(1-carboxyvinyl)-3-phosphoshikimate | r | |
2.5.1.19 | phosphoenolpyruvate + 3-phosphoshikimate | involved in aromatic acid biosynthesis | Bacillus subtilis | phosphate + 5-enolpyruvylshikimate 3-phosphate | - |
? |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.5.1.19 | 0.04 | - |
N-phosphonomethylglycine | H385L mutant enzyme | Bacillus subtilis | |
2.5.1.19 | 0.05 | - |
N-phosphonomethylglycine | R24D mutant enzyme | Bacillus subtilis | |
2.5.1.19 | 0.06 | - |
N-phosphonomethylglycine | wild-type enzyme | Bacillus subtilis | |
2.5.1.19 | 0.21 | - |
N-phosphonomethylglycine | P105S mutant enzyme | Bacillus subtilis |