Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Majumder, K.; Selvapandiyan, A.; Fattah, F.A.; Arora, N.; Ahmad, S.; Bhatnagar, R.K.
    5-Enolpyruvylshikimate-3-phosphate synthase of Bacillus subtilis is an allosteric enzyme. Analysis of Arg24 -> Asp, Pro105 -> Ser and His385 -> Lys mutations suggests a hidden phosphoenolpyruvate-binding site (1995), Eur. J. Biochem., 229, 99-106.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.5.1.19 NH4Cl maximal activation at 100 mM Bacillus subtilis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.19 expression of wild-type and R24D, P105S, H385L mutant enzymes in Escherichia coli Bacillus subtilis

Protein Variants

EC Number Protein Variants Comment Organism
2.5.1.19 H385L 0.2% of wild-type activity, 2fold activation at 100 mM NH4Cl Bacillus subtilis
2.5.1.19 H385N 6% of wild-type activity Bacillus subtilis
2.5.1.19 P105S 69% of wild-type activity, 8fold activation at 100 mM NH4Cl Bacillus subtilis
2.5.1.19 R24D 0.8% of wild-type activity, 2fold activation at 100 mM NH4Cl Bacillus subtilis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.19 N-phosphonomethylglycine 0.25 mM, 50% inhibition of NH4Cl activated enzyme, 75 mM, 50% inhibition of nonactivated enzyme Bacillus subtilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.19 0.09
-
phosphoenolpyruvate wild-type enzyme Bacillus subtilis
2.5.1.19 0.16
-
shikimate 3-phosphate H385 mutant enzyme Bacillus subtilis
2.5.1.19 0.2
-
phosphoenolpyruvate R24D mutant enzyme Bacillus subtilis
2.5.1.19 0.2
-
shikimate 3-phosphate R24D mutant enzyme Bacillus subtilis
2.5.1.19 0.33
-
shikimate 3-phosphate wild-type enzyme Bacillus subtilis
2.5.1.19 0.43
-
shikimate 3-phosphate P105S mutant enzyme Bacillus subtilis
2.5.1.19 0.59
-
phosphoenolpyruvate P105S mutant enzyme Bacillus subtilis
2.5.1.19 1
-
phosphoenolpyruvate H385L mutant enzyme Bacillus subtilis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.19 48000
-
mixture of monomers and dimers in the absence of NH4Cl, only monomers in the presence of NH4Cl, gel filtration Bacillus subtilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.19 phosphoenolpyruvate + 3-phosphoshikimate Bacillus subtilis involved in aromatic acid biosynthesis phosphate + 5-enolpyruvylshikimate 3-phosphate
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.19 Bacillus subtilis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.19 recombinant enzyme, ammonium sulfate Bacillus subtilis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.19 10.4
-
-
Bacillus subtilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.19 phosphoenolpyruvate + 3-phosphoshikimate
-
Bacillus subtilis phosphate + 5-enolpyruvylshikimate 3-phosphate i.e. 5-O-(1-carboxyvinyl)-3-phosphoshikimate r
2.5.1.19 phosphoenolpyruvate + 3-phosphoshikimate involved in aromatic acid biosynthesis Bacillus subtilis phosphate + 5-enolpyruvylshikimate 3-phosphate
-
?

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.5.1.19 0.04
-
N-phosphonomethylglycine H385L mutant enzyme Bacillus subtilis
2.5.1.19 0.05
-
N-phosphonomethylglycine R24D mutant enzyme Bacillus subtilis
2.5.1.19 0.06
-
N-phosphonomethylglycine wild-type enzyme Bacillus subtilis
2.5.1.19 0.21
-
N-phosphonomethylglycine P105S mutant enzyme Bacillus subtilis