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Literature summary extracted from

  • Rubin, J.L.; Gaines, C.G.; Jensen, R.A.
    Glyphosphate inhibition of 5-enolpyruvylshikimate 3-phosphate synthase from suspension-cultured cells of Nicotiana silvestris (1984), Plant Physiol., 75, 839-845.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.19 N-phosphonomethylglycine 0.0183 mM, 50% inhibition, 0.5 mM 96% inhibition, competitive vs. phosphoenolpyruvate, uncompetitive vs. shikimate 3-phosphate at pH 7.0, mechanism; pH-dependent Nicotiana sylvestris

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.19 Nicotiana sylvestris
-
 cv. Speg et Comes, aneuploid cell line ANS-1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.19 DEAE-cellulose, CM-cellulose, partial purification Nicotiana sylvestris

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.5.1.19 cell suspension culture
-
 Nicotiana sylvestris
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.19 16.9
-
-
 Nicotiana sylvestris

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.19 phosphoenolpyruvate + 3-phosphoshikimate
-
 Nicotiana sylvestris phosphate + 5-enolpyruvylshikimate 3-phosphate i.e. 5-O-(1-carboxyvinyl)-3-phosphoshikimate r

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.19 7 7.2 50% of maximal activity between pH 6.2 and pH 8.5 Nicotiana sylvestris

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.5.1.19 6.2 8.5 approx. half-maximal activity at pH 6.2 and 8.5 Nicotiana sylvestris

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.5.1.19 0.00125
-
 N-phosphonomethylglycine vs. phosphoenolpyruvate Nicotiana sylvestris
2.5.1.19 0.0183
-
 N-phosphonomethylglycine vs. shikimate 3-phosphate Nicotiana sylvestris