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Literature summary extracted from

  • Ream, J.E.; Steinrücken, H.C.; Porter, C.A.; Sikorski, J.A.
    Purificatioin and properties of 5-enolpyruvylshikimate-3-phosphate synthase from dark-grown seedlings of Sorghum bicolor (1988), Plant Physiol., 87, 232-238.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.5.1.19 Cl- at about 100 mM Sorghum bicolor
2.5.1.19 F- stimulation Sorghum bicolor
2.5.1.19 NO3- stimulation at low concentrations, inhibition above 15 mM Sorghum bicolor
2.5.1.19 SO42- slight stimulation, inhibition above 50 mM Sorghum bicolor

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.19 ammonium heptamolybdate 1.1 mM, 50% inhibition, no inhibition below 0.1 mM Sorghum bicolor
2.5.1.19 CaCl2 above 100 mM Sorghum bicolor
2.5.1.19 Cu2+ 5 mM, complete inhibition Sorghum bicolor
2.5.1.19 Fe2+ 5 mM, 42% inhibition Sorghum bicolor
2.5.1.19 Fe3+ 5 mM, 68% inhibition Sorghum bicolor
2.5.1.19 MgCl2 above 100 mM Sorghum bicolor
2.5.1.19 Mn2+ 5 mM, 42% inhibition Sorghum bicolor
2.5.1.19 N-phosphonomethylglycine
-
 Sorghum bicolor
2.5.1.19 Pb2+ 5 mM, 97% inhibition Sorghum bicolor
2.5.1.19 SO42- inhibition above 50 mM, slight stimulation below Sorghum bicolor
2.5.1.19 Zn2+ 5 mM, complete inhibition Sorghum bicolor

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.19 0.007
-
 3-phosphoshikimate
-
 Sorghum bicolor
2.5.1.19 0.008
-
 phosphoenolpyruvate
-
 Sorghum bicolor

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.19 CaCl2 stimulation at 20 mM, inhibition above 100 mM Sorghum bicolor
2.5.1.19 MgCl2 stimulation at 20 mM, inhibition above 100 mM Sorghum bicolor

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.19 57000
-
 gel filtration Sorghum bicolor

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.19 Sorghum bicolor
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.19 ammonium sulfate, DEAE-cellulose, hydroxyapatite, phenylaggarose, Sephacryl S-200, isozymes I, II and III Sorghum bicolor

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.19 21.1
-
-
 Sorghum bicolor

Storage Stability

EC Number Storage Stability Organism
2.5.1.19 -20°C, 40% v/v glycerol, at least 1 year, no loss of activity Sorghum bicolor

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.19 phosphoenolpyruvate + 3-phosphoshikimate
-
 Sorghum bicolor phosphate + 5-enolpyruvylshikimate 3-phosphate i.e. 5-O-(1-carboxyvinyl)-3-phosphoshikimate r

Subunits

EC Number Subunits Comment Organism
2.5.1.19 monomer 1 * 50600-51900, 3 isozymes, SDS-PAGE Sorghum bicolor

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.19 30
-
 assay at Sorghum bicolor

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.19 7.4
-
 Tris-HCl buffer preferred Sorghum bicolor

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.5.1.19 6.5 8.5 approx. half-maximal activity at pH 6.5 and 8.5 Sorghum bicolor

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.5.1.19 0.00016
-
 N-phosphonomethylglycine vs. phosphoenolpyruvate Sorghum bicolor