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Literature summary extracted from

  • Mousdale, D.M.; Coggins, J.R.
    Purification and properties of 5-enolpyruvylshikimate 3-phosphate synthase from sedlings of Pisum sativum L. (1984), Planta, 160, 78-83.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.19 N-phosphonomethylglycine
-
 Pisum sativum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.19 0.0052
-
 phosphoenolpyruvate
-
 Pisum sativum
2.5.1.19 0.0052
-
 5-enolpyruvylshikimate 3-phosphate
-
 Pisum sativum
2.5.1.19 0.0077
-
 3-phosphoshikimate
-
 Pisum sativum
2.5.1.19 3.7
-
 phosphate
-
 Pisum sativum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.19 44000
-
 gel filtration Pisum sativum
2.5.1.19 50000
-
 1 * 50000, SDS-PAGE Pisum sativum

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.19 Pisum sativum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.19
-
 Pisum sativum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.5.1.19 seedling shoot tissue Pisum sativum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.19 5.67
-
 phosphate + 5-enolpyruvylshikimate 3-phosphate Pisum sativum
2.5.1.19 11.52
-
 forward reaction, i.e. phosphoenolpyruvate + 3-phosphoshikimate Pisum sativum

Storage Stability

EC Number Storage Stability Organism
2.5.1.19 -20°C, 50% glycerol, 10-15% loss of activity per month Pisum sativum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.19 phosphoenolpyruvate + 3-phosphoshikimate
-
 Pisum sativum phosphate + 5-enolpyruvylshikimate 3-phosphate i.e. 5-O-(1-carboxyvinyl)-3-phosphoshikimate r

Subunits

EC Number Subunits Comment Organism
2.5.1.19 monomer 1 * 50000, SDS-PAGE Pisum sativum

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.5.1.19 0.00008
-
 N-phosphonomethylglycine
-
 Pisum sativum