Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Steinrücken, H.C.; Amrhein, N.
    5-Enolpyruvylshikimate-3-phosphate synthase of Klebsiella pneumoniae 2. Inhibition by glyphosate [N-(phosphonomethyl)glycine] (1984), Eur. J. Biochem., 143, 351-357.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.19 3-Bromopyruvate glyphosate protects Klebsiella pneumoniae
2.5.1.19 N-ethylmaleimide glyphosate protects Klebsiella pneumoniae
2.5.1.19 N-phosphonomethylglycine competitive vs. phosphoenolpyruvate, noncompetitive vs. shikimate 3-phosphate, 5-enolpyruvylshikimate 3-phosphate and phosphate, not inhibited by non-herbicidal analogues of glyphosate, e.g. aminomethylphosphonic acid, glyphosine, i.e. bis-N-(phosphonomethyl)glycine, or iminodiacetic acid; pH-dependent Klebsiella pneumoniae
2.5.1.19 Phenylglyoxal glyphosate protects Klebsiella pneumoniae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.19 0.017
-
 phosphoenolpyruvate in the presence of glyphosate Klebsiella pneumoniae

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.19 Klebsiella pneumoniae
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.19 phosphoenolpyruvate + 3-phosphoshikimate
-
 Klebsiella pneumoniae phosphate + 5-enolpyruvylshikimate 3-phosphate i.e. 5-O-(1-carboxyvinyl)-3-phosphoshikimate r

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.19 30
-
 assay at Klebsiella pneumoniae

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.5.1.19 0.001
-
 N-phosphonomethylglycine vs. phosphoenolpyruvate Klebsiella pneumoniae
2.5.1.19 0.008
-
 N-phosphonomethylglycine vs. 5-enolpyruvylshikimate 3-phosphate Klebsiella pneumoniae
2.5.1.19 0.027
-
 N-phosphonomethylglycine vs. phosphate Klebsiella pneumoniae
2.5.1.19 0.22
-
 N-phosphonomethylglycine vs. shikimate 3-phosphate Klebsiella pneumoniae