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Literature summary extracted from

  • Steinrücken, H.C.; Amrhein, N.
    5-Enolpyruvylshikimate-3-phosphate synthase of Klebsiella pneumoniae. 1. Purification and properties (1984), Eur. J. Biochem., 143, 341-349.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.5.1.19 Br- stimulation at low concentrations, inhibition above 200 mM Klebsiella pneumoniae
2.5.1.19 Cl- activation up to 100 mM, inhibition above Klebsiella pneumoniae
2.5.1.19 F- stimulation Klebsiella pneumoniae
2.5.1.19 I- stimulation at low concentrations, inhibition above 100 mM Klebsiella pneumoniae
2.5.1.19 NH4Cl stimulation, inhibition above 300 mM Klebsiella pneumoniae
2.5.1.19 NO3- stimulation at low concentrations, inhibition above 15 mM Klebsiella pneumoniae

General Stability

EC Number General Stability Organism
2.5.1.19 EDTA stabilizes Klebsiella pneumoniae
2.5.1.19 freezing inactivates, Polybuffer 74, operating range pH 7-4, 1 mM dithioerythritol stabilize Klebsiella pneumoniae

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.19 5-enolpyruvylshikimate 3-phosphate product inhibition Klebsiella pneumoniae
2.5.1.19 Al3+ 10 mM, 37% inhibition Klebsiella pneumoniae
2.5.1.19 Br- inhibition above 200 mM, stimulation below Klebsiella pneumoniae
2.5.1.19 Cl- inhibition above 400 mM, stimulation below Klebsiella pneumoniae
2.5.1.19 Co3+ 10 mM, 25% inhibition Klebsiella pneumoniae
2.5.1.19 Cu2+ 10 mM, 90-95% inhibition Klebsiella pneumoniae
2.5.1.19 Fe2+ 10 mM, complete inhibition Klebsiella pneumoniae
2.5.1.19 Fe3+ 10 mM, 60% inhibition Klebsiella pneumoniae
2.5.1.19 I- inhibition above 100 mM, stimulation below Klebsiella pneumoniae
2.5.1.19 La3+ 10 mM, complete inhibition Klebsiella pneumoniae
2.5.1.19 Mn2+ 10 mM, 16% inhibition Klebsiella pneumoniae
2.5.1.19 molybdate 10 mM, complete inhibition Klebsiella pneumoniae
2.5.1.19 NH4Cl inhibition above 300 mM, stimulation below Klebsiella pneumoniae
2.5.1.19 NO3- inhibition above 150 mM, stimulation below Klebsiella pneumoniae
2.5.1.19 Pb2+ 10 mM, 90-95% inhibition Klebsiella pneumoniae
2.5.1.19 phosphoenolpyruvate
-
 Klebsiella pneumoniae
2.5.1.19 shikimate 3-phosphate product inhibition Klebsiella pneumoniae
2.5.1.19 Zn2+ 10 mM, 90-95% inhibition Klebsiella pneumoniae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.19 additional information
-
 additional information kinetic study Klebsiella pneumoniae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.5.1.19 soluble
-
 Klebsiella pneumoniae
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.19 additional information not activated by Ba2+, Ca2+, Mg2+, Ni2+ or Sr2+ Klebsiella pneumoniae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.19 32400
-
 gel filtration Klebsiella pneumoniae
2.5.1.19 42900
-
 1 * 42900, glyphosate-resistant enzyme, SDS-PAGE Klebsiella pneumoniae

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.19 Klebsiella pneumoniae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.19
-
 Klebsiella pneumoniae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.19 37.8
-
-
 Klebsiella pneumoniae

Storage Stability

EC Number Storage Stability Organism
2.5.1.19 -18°C, in Polybuffer 74 from Pharmacia, operating range pH 7-4, 1 mM dithioerythritol, at least 9 months Klebsiella pneumoniae
2.5.1.19 0-4°C, 2 months Klebsiella pneumoniae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.19 phosphoenolpyruvate + 3-phosphoshikimate
-
 Klebsiella pneumoniae phosphate + 5-enolpyruvylshikimate 3-phosphate i.e. 5-O-(1-carboxyvinyl)-3-phosphoshikimate r

Subunits

EC Number Subunits Comment Organism
2.5.1.19 monomer 1 * 42900, glyphosate-resistant enzyme, SDS-PAGE Klebsiella pneumoniae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.19 30
-
 assay at Klebsiella pneumoniae
2.5.1.19 60
-
 wild-type enzyme Klebsiella pneumoniae

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.5.1.19 45 62 approx. half-maximal activity at 45°C and 62°C Klebsiella pneumoniae

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.5.1.19 60
-
 rapid inactivation above Klebsiella pneumoniae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.19 additional information
-
 pI: 4.6, wild-type enzyme Klebsiella pneumoniae
2.5.1.19 5.6
-
 phosphate + 5-enolpyruvylshikimate 3-phosphate, 2 maxima at pH 5.6 and pH 7.6 Klebsiella pneumoniae
2.5.1.19 7.6
-
 phosphate + 5-enolpyruvylshikimate 3-phosphate, 2 maxima, at pH 5.6 and pH 7.6 Klebsiella pneumoniae

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.5.1.19 5 8.5 approx. half-maximal activity at pH 5.0 and 8.5 Klebsiella pneumoniae
2.5.1.19 5.5 8.7 approx. half-maximal activity at pH 5.5 and 8.7, phosphate + 5-enolpyruvylshikimate 3-phosphate Klebsiella pneumoniae

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.5.1.19 0.0013
-
 5-enolpyruvylshikimate 3-phosphate
-
 Klebsiella pneumoniae
2.5.1.19 0.023
-
 shikimate 3-phosphate
-
 Klebsiella pneumoniae
2.5.1.19 0.044
-
 phosphoenolpyruvate
-
 Klebsiella pneumoniae