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Literature summary extracted from

  • Lewendon, A.; Coggins, J.R.
    Purification of 5-enolpyruvylshikimate 3-phosphate synthase from Escherichia coli (1983), Biochem. J., 213, 187-191.
    View publication on PubMedView publication on EuropePMC

General Stability

EC Number General Stability Organism
2.5.1.19 dithiothreitol, 0.4 mM, stabilizes during purification Escherichia coli
2.5.1.19 glycerol, 50% v/v, stabilizes Escherichia coli

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.5.1.19 soluble
-
 Escherichia coli
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.19 49000
-
 1 * 49000 Escherichia coli
2.5.1.19 55000
-
 gel filtration Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.19 phosphoenolpyruvate + 3-phosphoshikimate Escherichia coli involved in chorismate biosynthesis phosphate + 5-enolpyruvylshikimate 3-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.19 Escherichia coli
-
 K-12
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.19
-
 Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.19 18
-
-
 Escherichia coli
2.5.1.19 62
-
-
 Escherichia coli

Storage Stability

EC Number Storage Stability Organism
2.5.1.19 -20°C, 50 mM Tris-HCl, pH 7.5, 50 mM KCl, 0.4 mM dithiothreitol, 50% v/v glycerol, at least 4 months, no loss of activity Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.19 phosphoenolpyruvate + 3-phosphoshikimate transfers enolpyruvate group from phosphoenolpyruvate to 5-hydroxyl group of 3-phosphoshikimate Escherichia coli phosphate + 5-enolpyruvylshikimate 3-phosphate i.e. 5-O-(1-carboxyvinyl)-3-phosphoshikimate r
2.5.1.19 phosphoenolpyruvate + 3-phosphoshikimate involved in chorismate biosynthesis Escherichia coli phosphate + 5-enolpyruvylshikimate 3-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.19 monomer 1 * 49000 Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.19 25
-
 assay at Escherichia coli