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Literature summary extracted from

  • Montero, C.; Llorente, P.
    Artemia purine phosphoribosyltransferases. Purification and characterization (1991), Biochem. J., 275, 327-334.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.2.7 AMP competitive against 5-phospho-alpha-D-ribose 1-diphosphate Artemia sp.
2.4.2.7 Ba2+
-
 Artemia sp.
2.4.2.7 Ca2+ strong Artemia sp.
2.4.2.7 guanine noncompetitive against 5-phosphoribose 1-diphosphate Artemia sp.
2.4.2.8 Ba2+
-
 Artemia sp.
2.4.2.8 Ca2+ strong Artemia sp.
2.4.2.8 GMP mixed inhibition Artemia sp.
2.4.2.8 guanine substrate inhibition Artemia sp.
2.4.2.8 hypoxanthine substrate inhibition Artemia sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.7 0.002
-
 adenine
-
 Artemia sp.
2.4.2.7 0.03
-
 5-phospho-alpha-D-ribose 1-diphosphate
-
 Artemia sp.
2.4.2.8 0.001
-
 guanine below Artemia sp.
2.4.2.8 0.001
-
 hypoxanthine below Artemia sp.
2.4.2.8 0.015
-
 5-phospho-alpha-D-ribose 1-diphosphate
-
 Artemia sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.2.7 cytosol
-
 Artemia sp. 5829
-
2.4.2.8 cytosol
-
 Artemia sp. 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.2.7 Ca2+ inhibition Artemia sp.
2.4.2.7 Mg2+ required Artemia sp.
2.4.2.7 Mg2+ 0.15-0.18 mM Artemia sp.
2.4.2.7 Mn2+ Km: 0.04-0.05 Artemia sp.
2.4.2.7 Mn2+ activity depends on presence of divalent cations, Mn2+ or Mg2+ activates Artemia sp.
2.4.2.7 Zn2+ activates, Km: 0.045-0.05 mM Artemia sp.
2.4.2.8 Mg2+ required Artemia sp.
2.4.2.8 Mg2+ maximal activity at: 1 mM Artemia sp.
2.4.2.8 Mg2+ Km: 0.04-0.05 mM Artemia sp.
2.4.2.8 Mn2+ can replace Mg2+ Artemia sp.
2.4.2.8 Mn2+ Km: 0.015-0.020 mM Artemia sp.
2.4.2.8 Zn2+ activates, can replace Mg2+, Km: 0.066-0.075 mM Artemia sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.2.7 15000
-
 2 * 15000, SDS-PAGE Artemia sp.
2.4.2.7 28000
-
 gel filtration Artemia sp.
2.4.2.8 19000
-
 4 * 19000, SDS-PAGE Artemia sp.
2.4.2.8 66000
-
 gel filtration Artemia sp.

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.7 Artemia sp.
-
-
-
2.4.2.8 Artemia sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.7
-
 Artemia sp.
2.4.2.8
-
 Artemia sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.2.7 cyst
-
 Artemia sp.
-
2.4.2.7 nauplius
-
 Artemia sp.
-
2.4.2.8 cyst
-
 Artemia sp.
-
2.4.2.8 nauplius
-
 Artemia sp.
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.2.7 14.2
-
 purified enzyme Artemia sp.
2.4.2.8 0.65
-
 purified enzyme Artemia sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.7 adenine + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Artemia sp. AMP + diphosphate
-
 ?
2.4.2.8 guanine + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Artemia sp. GMP + diphosphate
-
 ?
2.4.2.8 hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Artemia sp. IMP + diphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.2.7 dimer 2 * 15000, SDS-PAGE Artemia sp.
2.4.2.8 tetramer 4 * 19000, SDS-PAGE Artemia sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.2.7 37
-
 assay at Artemia sp.
2.4.2.8 37
-
 assay at Artemia sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.2.7 8 9
-
 Artemia sp.
2.4.2.8 7
-
 2 buffer-independent pH-optima: pH 7.0 and pH 9.5 Artemia sp.
2.4.2.8 9.5
-
 2 buffer-independent pH-optima: pH 7.0 and pH 9.5 Artemia sp.

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.4.2.8 0.01
-
 guanine
-
 Artemia sp.
2.4.2.8 0.08
-
 hypoxanthine
-
 Artemia sp.