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Literature summary extracted from

  • Moffatt, B.A.; Somerville, C.R.
    Purification of adenine phosphoribosyltransferase from Brassica juncea (1990), Arch. Biochem. Biophys., 283, 484-490.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.2.7 N-ethylmaleimide
-
 Brassica juncea
2.4.2.7 p-hydroxymercuribenzoate DTT Brassica juncea
2.4.2.7 sulfhydryl reagents
-
 Brassica juncea

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.7 0.0038
-
 adenine
-
 Brassica juncea
2.4.2.7 0.015
-
 5-phospho-alpha-D-ribose 1-diphosphate
-
 Brassica juncea

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.2.7 Mg2+ required Brassica juncea
2.4.2.7 Mg2+ 3 mM at 60°C, at 37°C activity varies little in the range 3-50 mM MgCl2 Brassica juncea

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.2.7 27000
-
 2 * 27000, SDS-PAGE Brassica juncea
2.4.2.7 54000
-
 gel filtration Brassica juncea

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.7 Brassica juncea
-
 cv. Green Wave
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.7
-
 Brassica juncea

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.2.7 leaf
-
 Brassica juncea
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.2.7 9.15
-
-
 Brassica juncea

Storage Stability

EC Number Storage Stability Organism
2.4.2.7 -70°C, 50 mM Tris-HCl, pH 7.4, 5 mM MgCl2, 10 mM KCl, 10% glycerol, stable Brassica juncea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.7 adenine + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Brassica juncea AMP + diphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.2.7 dimer 2 * 27000, SDS-PAGE Brassica juncea

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.2.7 37
-
 assay at Brassica juncea
2.4.2.7 60
-
-
 Brassica juncea

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.2.7 9.2
-
-
 Brassica juncea