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Literature summary extracted from

  • Chen, C.M.; Melitz, D.K.; Clough, F.W.
    Metabolism of cytokinin: phosphoribosylation of cytokinin bases by adenine phosphoribosyltransferase from wheat germ (1982), Arch. Biochem. Biophys., 214, 634-641.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.2.7 Ca2+ activation, competitive to Mg2+ Triticum aestivum
2.4.2.7 Hg2+ complete inhibition at 5 mM Triticum aestivum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.7 0.074
-
 adenine
-
 Triticum aestivum
2.4.2.7 0.11
-
 N6-furfuryladenine
-
 Triticum aestivum
2.4.2.7 0.13
-
 N6-(DELTA2-isopentenyl)adenine
-
 Triticum aestivum
2.4.2.7 0.154
-
 N6-benzyladenine
-
 Triticum aestivum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.2.7 cytosol
-
 Triticum aestivum 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.2.7 Ca2+ activates Triticum aestivum
2.4.2.7 Mg2+ required Triticum aestivum
2.4.2.7 Mg2+ optimal concentration: 5 mM Triticum aestivum
2.4.2.7 Mn2+ activity depends on presence of divalent cations, Mn2+ or Mg2+ activates Triticum aestivum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.2.7 23000
-
 gel filtration Triticum aestivum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.2.7 adenine + 5-phospho-alpha-D-ribose 1-diphosphate Triticum aestivum may play a role in maintaining the supply of adequate levels of active cytokinin AMP + diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.7 Triticum aestivum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.7 partial Triticum aestivum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.2.7 germ
-
 Triticum aestivum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.2.7 0.0413
-
-
 Triticum aestivum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.7 adenine + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Triticum aestivum AMP + diphosphate
-
 ?
2.4.2.7 adenine + 5-phospho-alpha-D-ribose 1-diphosphate may play a role in maintaining the supply of adequate levels of active cytokinin Triticum aestivum AMP + diphosphate
-
 ?
2.4.2.7 benzyladenine + 5-phospho-alpha-D-ribose 1-diphosphate N6-benzyladenine Triticum aestivum benzyladenosine 5'-phosphate + diphosphate
-
 ?
2.4.2.7 isopentenyladenine + 5-phospho-alpha-D-ribose 1-diphosphate N6-(delta-isopentenyl)adenine Triticum aestivum isopentenyladenosine 5'-phosphate + diphosphate
-
 ?
2.4.2.7 additional information no substrate: hypoxanthine,guanine Triticum aestivum ?
-
 ?
2.4.2.7 N6-furfuryladenine + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Triticum aestivum N6-furfuryladenosine 5'-phosphate + diphosphate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.2.7 37
-
 assay at Triticum aestivum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.2.7 7.5
-
-
 Triticum aestivum