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Literature summary extracted from

  • Thomas, C.B.; Arnold, W.J.; Kelley, W.N.
    Human adenine phosphoribosyltransferase. Purification, subunit structure, and substrate specificity (1973), J. Biol. Chem., 248, 2529-2535.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.4.2.7 ammonium sulfate, 1 M, partially protects against inactivation during storage Homo sapiens
2.4.2.7 dithiothreitol, 10 mM, or dimethylsulfoxide, 5%, or 5-phospho-alpha-D-ribose 1-diphosphate, 1 mM, protects against inactivation during storage Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.2.7 ATP
-
 Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.2.7 Mg2+
-
 Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.2.7 11100
-
 3 * 11100, SDS-PAGE Homo sapiens
2.4.2.7 34000
-
 gel filtration, sucrose density gradient ultracentrifugation Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.7 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.7
-
 Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.2.7 erythrocyte
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.2.7 9.58
-
 purified enzyme Homo sapiens

Storage Stability

EC Number Storage Stability Organism
2.4.2.7 -70°C, purified enzyme, 5 mM MgCl2, 0.1 mM 5-phospho-alpha-D-ribose 1-diphosphate, stable for upt to 2 months Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.7 2,6-diaminopurine + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Homo sapiens 2,6-diaminopurine ribotide + diphosphate
-
 ?
2.4.2.7 4-amino-5-imidazolecarboxamide + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Homo sapiens 4-amino-5-imidazolecarboxamide ribotide + diphosphate
-
 ?
2.4.2.7 adenine + 5-phospho-alpha-D-ribose 1-diphosphate best acceptor substrate Homo sapiens AMP + diphosphate
-
 ?
2.4.2.7 additional information no donor substrates: D-ribose 5-phosphate, ribose 1-phosphate Homo sapiens ?
-
 ?
2.4.2.7 additional information at 0°C in the absence of Mg2+ but in presence of substrates the enzyme catalyzes a rapid and limited synthesis of AMP Homo sapiens ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.2.7 trimer 3 * 11100, SDS-PAGE Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.2.7 additional information
-
 at 0°C in the absence of Mg2+ but in presence of substrates enzyme catalyzes a rapid and limited synthesis of AMP Homo sapiens
2.4.2.7 37
-
 assay at Homo sapiens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.2.7 7.4 9.5 broad maximum Homo sapiens