BRENDA - Enzyme Database show

Purification and properties of the NAD+: protein ADP-ribosyltransferase responsible for the T4-phage-induced modification of the alpha subunit of DNA-dependent RNA polymerase of Escherichia coli

Skorko, R.; Zillig, W.; Rohrer, H.; Mailhammer, R.; Eur. J. Biochem. 79, 55-66 (1977)

Data extracted from this reference:

General Stability
EC Number
General Stability
Organism
2.4.2.31
unstable during dialysis, loss of activity can be strongly reduced by using buffers containing 40% glycerol
Escherichia virus T4
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.4.2.31
AMP
7.8 mM, decreases activity to 78% of the uninhibited control
Escherichia virus T4
2.4.2.31
nicotinamide
-
Escherichia virus T4
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.4.2.31
0.0143
-
NAD+
-
Escherichia virus T4
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.4.2.31
26000
-
gel filtration
Escherichia virus T4
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.4.2.31
Escherichia virus T4
-
Escherichia coli B/r infected with phage T4
-
Purification (Commentary)
EC Number
Commentary
Organism
2.4.2.31
-
Escherichia virus T4
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.4.2.31
additional information
-
-
Escherichia virus T4
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.2.31
polyarginine + NAD+
-
638106
Escherichia virus T4
nicotinamide + ?
-
-
-
ir
2.4.2.31
RNA polymerase + NAD+
alpha-subunit of RNA polymerase
638106
Escherichia virus T4
additional information
the amino acid carrying the ADP-ribosyl residue appears to be arginine
638106
Escherichia virus T4
ir
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.4.2.31
20
-
-
Escherichia virus T4
Temperature Range [°C]
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
2.4.2.31
20
37
20°C: optimum, 37°C: about 35% of maximal activity
Escherichia virus T4
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.4.2.31
7.5
-
-
Escherichia virus T4
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.4.2.31
6
9
pH 6: about 50% of maximal activity, pH 9: about 80% of maximal activity
Escherichia virus T4
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.4.2.31
4
-
nicotinamide
-
Escherichia virus T4
General Stability (protein specific)
EC Number
General Stability
Organism
2.4.2.31
unstable during dialysis, loss of activity can be strongly reduced by using buffers containing 40% glycerol
Escherichia virus T4
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.4.2.31
AMP
7.8 mM, decreases activity to 78% of the uninhibited control
Escherichia virus T4
2.4.2.31
nicotinamide
-
Escherichia virus T4
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.4.2.31
4
-
nicotinamide
-
Escherichia virus T4
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.4.2.31
0.0143
-
NAD+
-
Escherichia virus T4
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.4.2.31
26000
-
gel filtration
Escherichia virus T4
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.4.2.31
-
Escherichia virus T4
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.4.2.31
additional information
-
-
Escherichia virus T4
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.2.31
polyarginine + NAD+
-
638106
Escherichia virus T4
nicotinamide + ?
-
-
-
ir
2.4.2.31
RNA polymerase + NAD+
alpha-subunit of RNA polymerase
638106
Escherichia virus T4
additional information
the amino acid carrying the ADP-ribosyl residue appears to be arginine
638106
Escherichia virus T4
ir
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.4.2.31
20
-
-
Escherichia virus T4
Temperature Range [°C] (protein specific)
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
2.4.2.31
20
37
20°C: optimum, 37°C: about 35% of maximal activity
Escherichia virus T4
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.4.2.31
7.5
-
-
Escherichia virus T4
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.4.2.31
6
9
pH 6: about 50% of maximal activity, pH 9: about 80% of maximal activity
Escherichia virus T4