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Literature summary extracted from

  • Kang, K.R.; Wolff, E.C.; Park, M.H.; Folk, J.E.; Chung, S.I.
    Identification of YHR068w in Saccharomyces cerevisiae chromosome VIII as a gene for deoxyhypusine synthase. Expression and characterization of the enzyme (1995), J. Biol. Chem., 270, 18408-18412.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.46 0.0083
-
 spermidine yeast recombinant eIF-5Aa Saccharomyces cerevisiae
2.5.1.46 0.0125
-
 spermidine yeast recombinant eIF-5Ab Saccharomyces cerevisiae
2.5.1.46 0.56
-
 NAD+ yeast recombinant eIF-5Aa Saccharomyces cerevisiae
2.5.1.46 0.81
-
 NAD+ yeast recombinant eIF-5Ab Saccharomyces cerevisiae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.5.1.46 cytoplasm
-
 Saccharomyces cerevisiae 5737
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.46 43000
-
 4 * 43000, SDS-PAGE Saccharomyces cerevisiae
2.5.1.46 165000 172000 gel filtration sedimentation equilibrium centrifugation Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.46 Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.46
-
 Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.46 0.0131
-
-
 Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.46 spermidine reaction in absence of eIF5A-precursor Saccharomyces cerevisiae 1,3-diaminopropane + DELTA1-pyrroline
-
 ?
2.5.1.46 [eIF5A-precursor]-lysine + spermidine
-
 Saccharomyces cerevisiae [eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine i.e. [eIF5A-precursor]-N6-(4-aminobutyl)lysine ?

Subunits

EC Number Subunits Comment Organism
2.5.1.46 tetramer 4 * 43000, SDS-PAGE Saccharomyces cerevisiae
2.5.1.46 tetramer 4* 42998, MALDI mass spectrometry Saccharomyces cerevisiae

Cofactor

EC Number Cofactor Comment Organism Structure
2.5.1.46 NAD+
-
 Saccharomyces cerevisiae