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Literature summary extracted from

  • Joe, Y.A.; Wolff, E.C.; Park, M.H.
    Cloning and expression of human deoxyhypusine synthase cDNA: structure-function studies with the recombinant enzyme and mutant proteins (1995), J. Biol. Chem., 270, 22386-22392.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.46 expression in Escherichia coli, the deduced amino acid sequence shows a high degree of identity to that of yeast enzyme and to the known sequences of tryptic peptides from the rat and Neurospora enzymes Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.5.1.46 additional information variant recombinant proteins with i. a truncation of 48 or 97 NH2-terminal amino acids, ii. A truncation of 39 COOH-terminal amino acids, or iii. An internal deletion (ASp262-Ser317) are inactive. A chimeric protein consisting of the complete human sequence and 16 amino acids of the yeast sequence, Gln197-Asn212, not present in the human enzyme, inserted between Glu193 and Gln194 exhibit moderate activity Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.46 0.0006
-
 [eIF5A-precursor]-L-lysine
-
 Homo sapiens
2.5.1.46 0.0048
-
 NAD+
-
 Homo sapiens
2.5.1.46 0.0072
-
 spermidine
-
 Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.46 41000
-
 x * 41000, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.46 [eIF5A-precursor]-lysine + spermidine Homo sapiens first step in the biosynthesis of hypusine [eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.46 Homo sapiens P49366
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.46
-
 Homo sapiens
2.5.1.46 recombinant enzyme Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.46 0.015
-
-
 Homo sapiens
2.5.1.46 0.019
-
-
 Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.46 spermidine
-
 Homo sapiens 1,3-diaminopropane + DELTA1-pyrroline
-
 ?
2.5.1.46 [eIF5A-precursor]-L-lysine + spermidine
-
 Homo sapiens [eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine
-
 ?
2.5.1.46 [eIF5A-precursor]-lysine + spermidine
-
 Homo sapiens [eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine i.e. [eIF5A-precursor]-N6-(4-aminobutyl)lysine ?
2.5.1.46 [eIF5A-precursor]-lysine + spermidine first step in the biosynthesis of hypusine Homo sapiens [eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.46 ? x * 41000, SDS-PAGE Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
2.5.1.46 NAD+ strict requirement Homo sapiens
2.5.1.46 NADH
-
 Homo sapiens