BRENDA - Enzyme Database show

Trans-N-deoxyribosylase from Lactobacillus helveticus. Crystallization and properties

Uerkvitz, W.; Eur. J. Biochem. 23, 387-395 (1971)

Data extracted from this reference:

Crystallization (Commentary)
EC Number
Crystallization
Organism
2.4.2.6
microdiffusion by means of equilibrium dialysis is used to crystallize the enzyme
Lactobacillus helveticus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.4.2.6
82000
-
gel filtration
Lactobacillus helveticus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.4.2.6
Lactobacillus helveticus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.4.2.6
-
Lactobacillus helveticus
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.4.2.6
23.1
-
-
Lactobacillus helveticus
Storage Stability
EC Number
Storage Stability
Organism
2.4.2.6
-50°C, stable for several months either in the lyophilized form or disolved in freshly distilled water
Lactobacillus helveticus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.2.6
deoxycytidine + adenine
-
638046
Lactobacillus helveticus
cytosine + deoxyadenosine
-
-
-
r
2.4.2.6
deoxycytidine + guanine
-
638046
Lactobacillus helveticus
cytosine + deoxyguanosine
-
-
-
r
2.4.2.6
deoxycytidine + hypoxanthine
-
638046
Lactobacillus helveticus
cytosine + deoxyinosine
-
-
-
r
2.4.2.6
deoxyguanosine + adenine
-
638046
Lactobacillus helveticus
guanine + deoxyadenosine
-
-
-
r
2.4.2.6
deoxyinosine + adenine
-
638046
Lactobacillus helveticus
hypoxanthine + deoxyadenosine
-
-
-
r
2.4.2.6
deoxyuridine + adenine
-
638046
Lactobacillus helveticus
deoxyadenosine + uracil
-
-
-
?
2.4.2.6
deoxyuridine + guanine
-
638046
Lactobacillus helveticus
deoxyguanosine + uracil
-
-
-
?
2.4.2.6
deoxyuridine + hypoxanthine
-
638046
Lactobacillus helveticus
deoxyinosine + uracil
-
-
-
?
2.4.2.6
thymidine + adenine
-
638046
Lactobacillus helveticus
thymine + adenosine
-
-
-
?
2.4.2.6
thymidine + cytosine
-
638046
Lactobacillus helveticus
thymine + 2'-deoxycytidine
-
-
-
?
2.4.2.6
thymidine + guanine
-
638046
Lactobacillus helveticus
deoxyguanine + thymine
-
-
-
?
2.4.2.6
thymidine + hypoxanthine
-
638046
Lactobacillus helveticus
deoxyinosine + thymine
-
-
-
?
2.4.2.6
thymidine + uracil
-
638046
Lactobacillus helveticus
deoxyuridine + thymine
-
-
-
?
Crystallization (Commentary) (protein specific)
EC Number
Crystallization
Organism
2.4.2.6
microdiffusion by means of equilibrium dialysis is used to crystallize the enzyme
Lactobacillus helveticus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.4.2.6
82000
-
gel filtration
Lactobacillus helveticus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.4.2.6
-
Lactobacillus helveticus
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.4.2.6
23.1
-
-
Lactobacillus helveticus
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
2.4.2.6
-50°C, stable for several months either in the lyophilized form or disolved in freshly distilled water
Lactobacillus helveticus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.2.6
deoxycytidine + adenine
-
638046
Lactobacillus helveticus
cytosine + deoxyadenosine
-
-
-
r
2.4.2.6
deoxycytidine + guanine
-
638046
Lactobacillus helveticus
cytosine + deoxyguanosine
-
-
-
r
2.4.2.6
deoxycytidine + hypoxanthine
-
638046
Lactobacillus helveticus
cytosine + deoxyinosine
-
-
-
r
2.4.2.6
deoxyguanosine + adenine
-
638046
Lactobacillus helveticus
guanine + deoxyadenosine
-
-
-
r
2.4.2.6
deoxyinosine + adenine
-
638046
Lactobacillus helveticus
hypoxanthine + deoxyadenosine
-
-
-
r
2.4.2.6
deoxyuridine + adenine
-
638046
Lactobacillus helveticus
deoxyadenosine + uracil
-
-
-
?
2.4.2.6
deoxyuridine + guanine
-
638046
Lactobacillus helveticus
deoxyguanosine + uracil
-
-
-
?
2.4.2.6
deoxyuridine + hypoxanthine
-
638046
Lactobacillus helveticus
deoxyinosine + uracil
-
-
-
?
2.4.2.6
thymidine + adenine
-
638046
Lactobacillus helveticus
thymine + adenosine
-
-
-
?
2.4.2.6
thymidine + cytosine
-
638046
Lactobacillus helveticus
thymine + 2'-deoxycytidine
-
-
-
?
2.4.2.6
thymidine + guanine
-
638046
Lactobacillus helveticus
deoxyguanine + thymine
-
-
-
?
2.4.2.6
thymidine + hypoxanthine
-
638046
Lactobacillus helveticus
deoxyinosine + thymine
-
-
-
?
2.4.2.6
thymidine + uracil
-
638046
Lactobacillus helveticus
deoxyuridine + thymine
-
-
-
?