BRENDA - Enzyme Database show

Deoxyribosyl transfer catalysis with trans-N-deoxyribosylase. Kinetic study of purine(pyrimidine) to pyrimidine(purine) trans-N-deoxyribosylase

Danzin, C.; Cardinaud, R.; Eur. J. Biochem. 62, 365-372 (1976)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.4.2.6
adenine
-
Lactobacillus helveticus
2.4.2.6
cytosine
-
Lactobacillus helveticus
2.4.2.6
deoxyadenosine
linear competitive inhibition of deoxycytidine
Lactobacillus helveticus
2.4.2.6
deoxycytidine
-
Lactobacillus helveticus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.4.2.6
0.0073
-
adenine
reaction with deoxyinosine
Lactobacillus helveticus
2.4.2.6
0.008
-
adenine
reaction with deoxyguanosine
Lactobacillus helveticus
2.4.2.6
0.019
-
adenine
reaction with deoxycytidine
Lactobacillus helveticus
2.4.2.6
0.021
-
adenine
reaction with deoxyadenosine
Lactobacillus helveticus
2.4.2.6
0.073
-
cytosine
reaction with deoxyinosine
Lactobacillus helveticus
2.4.2.6
0.077
-
cytosine
reaction with deoxyguanosine
Lactobacillus helveticus
2.4.2.6
0.09
-
deoxycytidine
reaction with adenine
Lactobacillus helveticus
2.4.2.6
0.092
-
deoxyadenosine
reaction with adenine
Lactobacillus helveticus
2.4.2.6
0.095
-
deoxycytidine
reaction with cytosine
Lactobacillus helveticus
2.4.2.6
0.12
-
deoxyadenosine
reaction with cytosine
Lactobacillus helveticus
2.4.2.6
0.17
-
cytosine
reaction with deoxycytidine
Lactobacillus helveticus
2.4.2.6
0.22
-
cytosine
reaction with deoxyadenosine
Lactobacillus helveticus
2.4.2.6
0.346
-
deoxyguanosine
reaction with adenine
Lactobacillus helveticus
2.4.2.6
0.37
-
deoxyguanosine
reaction with cytosine
Lactobacillus helveticus
2.4.2.6
3.4
-
deoxyinosine
reaction with adenine
Lactobacillus helveticus
2.4.2.6
3.5
-
deoxyinosine
reaction with cytosine
Lactobacillus helveticus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.4.2.6
Lactobacillus helveticus
-
-
-
Reaction
EC Number
Reaction
Commentary
Organism
2.4.2.6
2-deoxy-D-ribosyl-base1 + base2 = 2-deoxy-D-ribosyl-base2 + base1
ping-pong bi bi mechanism
Lactobacillus helveticus
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.4.2.6
0.8
-
reaction with deoxyinosine and adenine
Lactobacillus helveticus
2.4.2.6
7.75
-
reaction with thymidine and cytosine
Lactobacillus helveticus
2.4.2.6
43
-
reaction with deoxycytidine and adenine
Lactobacillus helveticus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.2.6
deoxyadenosine + adenine
-
638045
Lactobacillus helveticus
adenine + deoxyadenosine
-
-
-
?
2.4.2.6
deoxyadenosine + cytosine
-
638045
Lactobacillus helveticus
adenine + deoxycytidine
-
-
-
r
2.4.2.6
deoxycytidine + adenine
-
638045
Lactobacillus helveticus
cytosine + deoxyadenosine
-
-
-
r
2.4.2.6
deoxyguanosine + adenine
-
638045
Lactobacillus helveticus
guanine + deoxyadenosine
-
-
-
?
2.4.2.6
deoxyguanosine + cytosine
-
638045
Lactobacillus helveticus
guanine + deoxycytidine
-
-
-
r
2.4.2.6
deoxyinosine + adenine
-
638045
Lactobacillus helveticus
hypoxanthine + deoxyadenosine
-
-
-
r
2.4.2.6
deoxyinosine + cytosine
-
638045
Lactobacillus helveticus
hypoxanthine + deoxycytidine
-
-
-
?
2.4.2.6
thymidine + cytosine
-
638045
Lactobacillus helveticus
thymine + 2'-deoxycytidine
-
-
-
?
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.4.2.6
0.021
-
adenine
-
Lactobacillus helveticus
2.4.2.6
0.092
-
deoxyadenosine
-
Lactobacillus helveticus
2.4.2.6
0.095
-
deoxycytidine
-
Lactobacillus helveticus
2.4.2.6
0.17
-
cytosine
-
Lactobacillus helveticus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.4.2.6
adenine
-
Lactobacillus helveticus
2.4.2.6
cytosine
-
Lactobacillus helveticus
2.4.2.6
deoxyadenosine
linear competitive inhibition of deoxycytidine
Lactobacillus helveticus
2.4.2.6
deoxycytidine
-
Lactobacillus helveticus
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.4.2.6
0.021
-
adenine
-
Lactobacillus helveticus
2.4.2.6
0.092
-
deoxyadenosine
-
Lactobacillus helveticus
2.4.2.6
0.095
-
deoxycytidine
-
Lactobacillus helveticus
2.4.2.6
0.17
-
cytosine
-
Lactobacillus helveticus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.4.2.6
0.0073
-
adenine
reaction with deoxyinosine
Lactobacillus helveticus
2.4.2.6
0.008
-
adenine
reaction with deoxyguanosine
Lactobacillus helveticus
2.4.2.6
0.019
-
adenine
reaction with deoxycytidine
Lactobacillus helveticus
2.4.2.6
0.021
-
adenine
reaction with deoxyadenosine
Lactobacillus helveticus
2.4.2.6
0.073
-
cytosine
reaction with deoxyinosine
Lactobacillus helveticus
2.4.2.6
0.077
-
cytosine
reaction with deoxyguanosine
Lactobacillus helveticus
2.4.2.6
0.09
-
deoxycytidine
reaction with adenine
Lactobacillus helveticus
2.4.2.6
0.092
-
deoxyadenosine
reaction with adenine
Lactobacillus helveticus
2.4.2.6
0.095
-
deoxycytidine
reaction with cytosine
Lactobacillus helveticus
2.4.2.6
0.12
-
deoxyadenosine
reaction with cytosine
Lactobacillus helveticus
2.4.2.6
0.17
-
cytosine
reaction with deoxycytidine
Lactobacillus helveticus
2.4.2.6
0.22
-
cytosine
reaction with deoxyadenosine
Lactobacillus helveticus
2.4.2.6
0.346
-
deoxyguanosine
reaction with adenine
Lactobacillus helveticus
2.4.2.6
0.37
-
deoxyguanosine
reaction with cytosine
Lactobacillus helveticus
2.4.2.6
3.4
-
deoxyinosine
reaction with adenine
Lactobacillus helveticus
2.4.2.6
3.5
-
deoxyinosine
reaction with cytosine
Lactobacillus helveticus
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.4.2.6
0.8
-
reaction with deoxyinosine and adenine
Lactobacillus helveticus
2.4.2.6
7.75
-
reaction with thymidine and cytosine
Lactobacillus helveticus
2.4.2.6
43
-
reaction with deoxycytidine and adenine
Lactobacillus helveticus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.2.6
deoxyadenosine + adenine
-
638045
Lactobacillus helveticus
adenine + deoxyadenosine
-
-
-
?
2.4.2.6
deoxyadenosine + cytosine
-
638045
Lactobacillus helveticus
adenine + deoxycytidine
-
-
-
r
2.4.2.6
deoxycytidine + adenine
-
638045
Lactobacillus helveticus
cytosine + deoxyadenosine
-
-
-
r
2.4.2.6
deoxyguanosine + adenine
-
638045
Lactobacillus helveticus
guanine + deoxyadenosine
-
-
-
?
2.4.2.6
deoxyguanosine + cytosine
-
638045
Lactobacillus helveticus
guanine + deoxycytidine
-
-
-
r
2.4.2.6
deoxyinosine + adenine
-
638045
Lactobacillus helveticus
hypoxanthine + deoxyadenosine
-
-
-
r
2.4.2.6
deoxyinosine + cytosine
-
638045
Lactobacillus helveticus
hypoxanthine + deoxycytidine
-
-
-
?
2.4.2.6
thymidine + cytosine
-
638045
Lactobacillus helveticus
thymine + 2'-deoxycytidine
-
-
-
?