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Literature summary extracted from

  • Iwai, K.; Taguchi, H.
    Crystallization and properties of quinolinate phosphoribosyltransferase from hog liver (1980), Methods Enzymol., 66, 96-101.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.4.2.19
-
 Sus scrofa

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.2.19 ADP 2 mM, 25% inhibition Sus scrofa
2.4.2.19 ATP 2 mM, 82% inhibition Sus scrofa
2.4.2.19 Cu2+ 1 mM, 87% inhibition Sus scrofa
2.4.2.19 Fe2+ 1 mM, 93% inhibition Sus scrofa
2.4.2.19 Monoiodoacetic acid 5 mM, 50% inhibition Sus scrofa
2.4.2.19 PCMB 0.05 mM, complete inhibition Sus scrofa
2.4.2.19 Phthalic acid competitive to quinolinate Sus scrofa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.19 0.12
-
 quinolinate
-
 Sus scrofa
2.4.2.19 0.18
-
 5-phospho-alpha-D-ribose 1-diphosphate
-
 Sus scrofa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.2.19 Mg2+ optimal Mg2+ concentration: 1 mM Sus scrofa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.2.19 33500
-
 x * 33500, SDS-PAGE Sus scrofa
2.4.2.19 202000
-
 equilibrium sedimentation Sus scrofa
2.4.2.19 210000
-
 gel filtration Sus scrofa

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.19 Sus scrofa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.19
-
 Sus scrofa

Reaction

EC Number Reaction Comment Organism Reaction ID
2.4.2.19 beta-nicotinate D-ribonucleotide + diphosphate + CO2 = pyridine-2,3-dicarboxylate + 5-phospho-alpha-D-ribose 1-diphosphate formation of a ternary complex consisting the enzyme, pyridine-2,3-dicarboxylate and 5-phospho-alpha-D-ribose 1-diphosphate Sus scrofa
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.2.19 liver
-
 Sus scrofa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.2.19 0.05169
-
-
 Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.19 pyridine-2,3-dicarboxylate + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Sus scrofa nicotinate D-ribonucleotide + diphosphate + CO2 i.e. beta-niacin mononucleotide ?

Subunits

EC Number Subunits Comment Organism
2.4.2.19 ? x * 33500, SDS-PAGE Sus scrofa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.2.19 6.1
-
-
 Sus scrofa

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.4.2.19 5.5 10
-
 Sus scrofa

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.4.2.19 0.17
-
 Phthalic acid
-
 Sus scrofa