BRENDA - Enzyme Database show

Adenosine diphosphate ribose transferase from baby-hamster kidney cells (BHK-21/C13). Characterization of the reaction and product

Furneaux, H.M.; Pearson, C.K.; Biochem. J. 187, 91-103 (1980)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.4.2.30
ATP
5-10 mM, 20-30% stimulation
Mesocricetus auratus
General Stability
EC Number
General Stability
Organism
2.4.2.30
unstable in absence of Mg2+
Mesocricetus auratus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.4.2.30
NEM
0.5 mM
Mesocricetus auratus
2.4.2.30
nicotinamide
-
Mesocricetus auratus
2.4.2.30
thymidine
-
Mesocricetus auratus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.4.2.30
additional information
-
additional information
kinetics for NAD+ utilization are not Michaelis-Menten type
Mesocricetus auratus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.4.2.30
cytosol
10% of the activity
Mesocricetus auratus
5829
-
2.4.2.30
nucleus
90% of the activity. Chromatin-bound
Mesocricetus auratus
5634
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.4.2.30
Mg2+
divalent cation required, Mg2+ preferred to Mn2+, optimal concentration 5 mM
Mesocricetus auratus
2.4.2.30
Mn2+
divalent cation required, Mg2+ preferred to Mn2+
Mesocricetus auratus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.4.2.30
Mesocricetus auratus
-
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.4.2.30
BHK-21 cell
-
Mesocricetus auratus
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.2.30
NAD+ + (ADP-D-ribosyl)n-acceptor
-
637995
Mesocricetus auratus
nicotinamide + (ADP-D-ribosyl)n+1-acceptor
-
-
-
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.4.2.30
25
-
-
Mesocricetus auratus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.4.2.30
8
-
-
Mesocricetus auratus
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.4.2.30
ATP
5-10 mM, 20-30% stimulation
Mesocricetus auratus
General Stability (protein specific)
EC Number
General Stability
Organism
2.4.2.30
unstable in absence of Mg2+
Mesocricetus auratus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.4.2.30
NEM
0.5 mM
Mesocricetus auratus
2.4.2.30
nicotinamide
-
Mesocricetus auratus
2.4.2.30
thymidine
-
Mesocricetus auratus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.4.2.30
additional information
-
additional information
kinetics for NAD+ utilization are not Michaelis-Menten type
Mesocricetus auratus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.4.2.30
cytosol
10% of the activity
Mesocricetus auratus
5829
-
2.4.2.30
nucleus
90% of the activity. Chromatin-bound
Mesocricetus auratus
5634
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.4.2.30
Mg2+
divalent cation required, Mg2+ preferred to Mn2+, optimal concentration 5 mM
Mesocricetus auratus
2.4.2.30
Mn2+
divalent cation required, Mg2+ preferred to Mn2+
Mesocricetus auratus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.4.2.30
BHK-21 cell
-
Mesocricetus auratus
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.2.30
NAD+ + (ADP-D-ribosyl)n-acceptor
-
637995
Mesocricetus auratus
nicotinamide + (ADP-D-ribosyl)n+1-acceptor
-
-
-
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.4.2.30
25
-
-
Mesocricetus auratus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.4.2.30
8
-
-
Mesocricetus auratus