BRENDA - Enzyme Database show

Purification and characterization of NAD+:ADP-ribosyltransferase (polymerizing) from Dictyostelium discoideum

Kofler, B.; Wallraff, E.; Herzog, H.; Schneider, R.; Auer, B.; Schweiger, M.; Biochem. J. 293, 275-281 (1993)
No PubMed abstract available

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.4.2.30
DNA
absolute requirement
Dictyostelium discoideum
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.4.2.30
2,3-dihydro-5-hydroxy-1,4-phthalazinedione
0.001 mM, 95% inhibition of the 116000 Da enzyme
Dictyostelium discoideum
2.4.2.30
3-aminobenzamide
-
Dictyostelium discoideum
2.4.2.30
3-Aminophthalhydrazide
0.1 mM, 98% inhibition of the 116000 Da enzyme
Dictyostelium discoideum
2.4.2.30
3-Guanidinobenzamide
0.1 mM, 71% inhibition of the 116000 Da enzyme
Dictyostelium discoideum
2.4.2.30
3-Hydroxybenzamide
0.1 mM, 89% inhibition of the 116000 Da enzyme
Dictyostelium discoideum
2.4.2.30
3-Methoxybenzamide
0.01 mM, 85% inhibition of the 116000 Da enzyme, 84% inhibition of the 90000 Da enzyme; 1 mM, 96% inhibition of the 116000 Da enzyme, 95% inhibition of the 90000 Da enzyme
Dictyostelium discoideum
2.4.2.30
Alpha-NAD+
0.5 mM, 40% inhibition of the 116000 Da enzyme, 44% inhibition of the 90000 Da enzyme
Dictyostelium discoideum
2.4.2.30
Caffeine
1 mM, 86% inhibition of the 116000 Da enzyme, 57% inhibition of the 90000 Da enzyme
Dictyostelium discoideum
2.4.2.30
GTP(gammaS)
in presence of Mg2+
Dictyostelium discoideum
2.4.2.30
KCl
100 mM, 85% inhibition of the 90000 Da enzyme
Dictyostelium discoideum
2.4.2.30
nicotinamide
1 mM, 96% inhibition of the 116000 Da enzyme, 95% inhibition of the 90000 Da enzyme
Dictyostelium discoideum
2.4.2.30
theophylline
1 mM, 68% inhibition of the 116000 Da enzyme, 39% inhibition of the 90000 Da enzyme
Dictyostelium discoideum
2.4.2.30
thymidine
1 mM, 94% inhibition of the 116000 Da enzyme, 88% inhibition of the 90000 Da enzyme
Dictyostelium discoideum
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.4.2.30
0.02
-
NAD+
90000 Da protein
Dictyostelium discoideum
2.4.2.30
0.077
-
NAD+
116000 Da protein
Dictyostelium discoideum
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.4.2.30
90000
-
1 * 90000, two active proteins are detected: MW 90000 Da and 116000 Da, SDS-PAGE
Dictyostelium discoideum
2.4.2.30
116000
-
1 * 116000, two active proteins are detected: MW 90000 Da and 116000 Da, SDS-PAGE
Dictyostelium discoideum
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.4.2.30
Dictyostelium discoideum
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.4.2.30
-
Dictyostelium discoideum
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.4.2.30
0.0017
-
-
Dictyostelium discoideum
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.2.30
NAD+ + (ADP-D-ribosyl)n-acceptor
poly(ADP-ribosyl)ation of histone H1
637993
Dictyostelium discoideum
nicotinamide + (ADP-D-ribosyl)n+1-acceptor
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
2.4.2.30
?
1 * 116000, two active proteins are detected: MW 90000 Da and 116000 Da, SDS-PAGE; 1 * 90000, two active proteins are detected: MW 90000 Da and 116000 Da, SDS-PAGE
Dictyostelium discoideum
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.4.2.30
5
-
116000 Da enzyme
Dictyostelium discoideum
2.4.2.30
10
-
90000 Da enzyme
Dictyostelium discoideum
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.4.2.30
8
-
in presence of DNA and histone H1
Dictyostelium discoideum
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.4.2.30
DNA
absolute requirement
Dictyostelium discoideum
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.4.2.30
2,3-dihydro-5-hydroxy-1,4-phthalazinedione
0.001 mM, 95% inhibition of the 116000 Da enzyme
Dictyostelium discoideum
2.4.2.30
3-aminobenzamide
-
Dictyostelium discoideum
2.4.2.30
3-Aminophthalhydrazide
0.1 mM, 98% inhibition of the 116000 Da enzyme
Dictyostelium discoideum
2.4.2.30
3-Guanidinobenzamide
0.1 mM, 71% inhibition of the 116000 Da enzyme
Dictyostelium discoideum
2.4.2.30
3-Hydroxybenzamide
0.1 mM, 89% inhibition of the 116000 Da enzyme
Dictyostelium discoideum
2.4.2.30
3-Methoxybenzamide
0.01 mM, 85% inhibition of the 116000 Da enzyme, 84% inhibition of the 90000 Da enzyme; 1 mM, 96% inhibition of the 116000 Da enzyme, 95% inhibition of the 90000 Da enzyme
Dictyostelium discoideum
2.4.2.30
Alpha-NAD+
0.5 mM, 40% inhibition of the 116000 Da enzyme, 44% inhibition of the 90000 Da enzyme
Dictyostelium discoideum
2.4.2.30
Caffeine
1 mM, 86% inhibition of the 116000 Da enzyme, 57% inhibition of the 90000 Da enzyme
Dictyostelium discoideum
2.4.2.30
GTP(gammaS)
in presence of Mg2+
Dictyostelium discoideum
2.4.2.30
KCl
100 mM, 85% inhibition of the 90000 Da enzyme
Dictyostelium discoideum
2.4.2.30
nicotinamide
1 mM, 96% inhibition of the 116000 Da enzyme, 95% inhibition of the 90000 Da enzyme
Dictyostelium discoideum
2.4.2.30
theophylline
1 mM, 68% inhibition of the 116000 Da enzyme, 39% inhibition of the 90000 Da enzyme
Dictyostelium discoideum
2.4.2.30
thymidine
1 mM, 94% inhibition of the 116000 Da enzyme, 88% inhibition of the 90000 Da enzyme
Dictyostelium discoideum
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.4.2.30
0.02
-
NAD+
90000 Da protein
Dictyostelium discoideum
2.4.2.30
0.077
-
NAD+
116000 Da protein
Dictyostelium discoideum
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.4.2.30
90000
-
1 * 90000, two active proteins are detected: MW 90000 Da and 116000 Da, SDS-PAGE
Dictyostelium discoideum
2.4.2.30
116000
-
1 * 116000, two active proteins are detected: MW 90000 Da and 116000 Da, SDS-PAGE
Dictyostelium discoideum
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.4.2.30
-
Dictyostelium discoideum
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.4.2.30
0.0017
-
-
Dictyostelium discoideum
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.2.30
NAD+ + (ADP-D-ribosyl)n-acceptor
poly(ADP-ribosyl)ation of histone H1
637993
Dictyostelium discoideum
nicotinamide + (ADP-D-ribosyl)n+1-acceptor
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.4.2.30
?
1 * 116000, two active proteins are detected: MW 90000 Da and 116000 Da, SDS-PAGE; 1 * 90000, two active proteins are detected: MW 90000 Da and 116000 Da, SDS-PAGE
Dictyostelium discoideum
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.4.2.30
5
-
116000 Da enzyme
Dictyostelium discoideum
2.4.2.30
10
-
90000 Da enzyme
Dictyostelium discoideum
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.4.2.30
8
-
in presence of DNA and histone H1
Dictyostelium discoideum