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Literature summary extracted from

  • Kofler, B.; Wallraff, E.; Herzog, H.; Schneider, R.; Auer, B.; Schweiger, M.
    Purification and characterization of NAD+:ADP-ribosyltransferase (polymerizing) from Dictyostelium discoideum (1993), Biochem. J., 293, 275-281.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.2.30 DNA absolute requirement Dictyostelium discoideum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.2.30 2,3-dihydro-5-hydroxy-1,4-phthalazinedione 0.001 mM, 95% inhibition of the 116000 Da enzyme Dictyostelium discoideum
2.4.2.30 3-aminobenzamide
-
 Dictyostelium discoideum
2.4.2.30 3-Aminophthalhydrazide 0.1 mM, 98% inhibition of the 116000 Da enzyme Dictyostelium discoideum
2.4.2.30 3-Guanidinobenzamide 0.1 mM, 71% inhibition of the 116000 Da enzyme Dictyostelium discoideum
2.4.2.30 3-Hydroxybenzamide 0.1 mM, 89% inhibition of the 116000 Da enzyme Dictyostelium discoideum
2.4.2.30 3-Methoxybenzamide 0.01 mM, 85% inhibition of the 116000 Da enzyme, 84% inhibition of the 90000 Da enzyme; 1 mM, 96% inhibition of the 116000 Da enzyme, 95% inhibition of the 90000 Da enzyme Dictyostelium discoideum
2.4.2.30 Alpha-NAD+ 0.5 mM, 40% inhibition of the 116000 Da enzyme, 44% inhibition of the 90000 Da enzyme Dictyostelium discoideum
2.4.2.30 Caffeine 1 mM, 86% inhibition of the 116000 Da enzyme, 57% inhibition of the 90000 Da enzyme Dictyostelium discoideum
2.4.2.30 GTP(gammaS) in presence of Mg2+ Dictyostelium discoideum
2.4.2.30 KCl 100 mM, 85% inhibition of the 90000 Da enzyme Dictyostelium discoideum
2.4.2.30 nicotinamide 1 mM, 96% inhibition of the 116000 Da enzyme, 95% inhibition of the 90000 Da enzyme Dictyostelium discoideum
2.4.2.30 theophylline 1 mM, 68% inhibition of the 116000 Da enzyme, 39% inhibition of the 90000 Da enzyme Dictyostelium discoideum
2.4.2.30 thymidine 1 mM, 94% inhibition of the 116000 Da enzyme, 88% inhibition of the 90000 Da enzyme Dictyostelium discoideum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.30 0.02
-
 NAD+ 90000 Da protein Dictyostelium discoideum
2.4.2.30 0.077
-
 NAD+ 116000 Da protein Dictyostelium discoideum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.2.30 90000
-
 1 * 90000, two active proteins are detected: MW 90000 Da and 116000 Da, SDS-PAGE Dictyostelium discoideum
2.4.2.30 116000
-
 1 * 116000, two active proteins are detected: MW 90000 Da and 116000 Da, SDS-PAGE Dictyostelium discoideum

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.30 Dictyostelium discoideum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.30
-
 Dictyostelium discoideum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.2.30 0.0017
-
-
 Dictyostelium discoideum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.30 NAD+ + (ADP-D-ribosyl)n-acceptor poly(ADP-ribosyl)ation of histone H1 Dictyostelium discoideum nicotinamide + (ADP-D-ribosyl)n+1-acceptor
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.2.30 ? 1 * 90000, two active proteins are detected: MW 90000 Da and 116000 Da, SDS-PAGE Dictyostelium discoideum
2.4.2.30 ? 1 * 116000, two active proteins are detected: MW 90000 Da and 116000 Da, SDS-PAGE Dictyostelium discoideum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.2.30 5
-
 116000 Da enzyme Dictyostelium discoideum
2.4.2.30 10
-
 90000 Da enzyme Dictyostelium discoideum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.2.30 8
-
 in presence of DNA and histone H1 Dictyostelium discoideum