BRENDA - Enzyme Database show

ADP-ribosyltransferase from Helix pomatia. Purification and characterization

Burtscher, H.J.; Klocker, H.; Schneider, R.; Auer, B.; Hirsch-Kauffmann, M.; Schweiger, M.; Biochem. J. 248, 859-864 (1987)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.4.2.30
3-aminobenzamide
1 mM, 98% inhibition
Helix pomatia
2.4.2.30
3-Methoxybenzamide
1 mM, 98% inhibition
Helix pomatia
2.4.2.30
Caffeine
1 mM, 23% inhibition
Helix pomatia
2.4.2.30
nicotinamide
1 mM, 91% inhibition
Helix pomatia
2.4.2.30
Theobromine
1 mM, 76% inhibition
Helix pomatia
2.4.2.30
theophylline
1 mM, 72% inhibition
Helix pomatia
2.4.2.30
thymidine
1 mM, 70% inhibition
Helix pomatia
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.4.2.30
0.0267
-
NAD+
-
Helix pomatia
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.4.2.30
Helix pomatia
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.4.2.30
partial
Helix pomatia
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.4.2.30
foot
-
Helix pomatia
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.4.2.30
0.0545
-
-
Helix pomatia
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.2.30
NAD+ + (ADP-D-ribosyl)n-acceptor
poly(ADP-ribosyl)ation of histone
637992
Helix pomatia
nicotinamide + (ADP-D-ribosyl)n+1-acceptor
-
-
-
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.4.2.30
18
-
-
Helix pomatia
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.4.2.30
8
-
-
Helix pomatia
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.4.2.30
3-aminobenzamide
1 mM, 98% inhibition
Helix pomatia
2.4.2.30
3-Methoxybenzamide
1 mM, 98% inhibition
Helix pomatia
2.4.2.30
Caffeine
1 mM, 23% inhibition
Helix pomatia
2.4.2.30
nicotinamide
1 mM, 91% inhibition
Helix pomatia
2.4.2.30
Theobromine
1 mM, 76% inhibition
Helix pomatia
2.4.2.30
theophylline
1 mM, 72% inhibition
Helix pomatia
2.4.2.30
thymidine
1 mM, 70% inhibition
Helix pomatia
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.4.2.30
0.0267
-
NAD+
-
Helix pomatia
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.4.2.30
partial
Helix pomatia
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.4.2.30
foot
-
Helix pomatia
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.4.2.30
0.0545
-
-
Helix pomatia
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.2.30
NAD+ + (ADP-D-ribosyl)n-acceptor
poly(ADP-ribosyl)ation of histone
637992
Helix pomatia
nicotinamide + (ADP-D-ribosyl)n+1-acceptor
-
-
-
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.4.2.30
18
-
-
Helix pomatia
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.4.2.30
8
-
-
Helix pomatia