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Literature summary extracted from

  • Asaoka, K.; Takahashi, K.
    Monkey glutathione S-aryltransferases. II. Properties of the major enzyme purified from the liver (1977), J. Biochem., 82, 1313-1323.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.18 2,4,6-Trinitrobenzenesulfonate
-
 Macaca mulatta
2.5.1.18 benzene hexachloride
-
 Macaca mulatta
2.5.1.18 Cd2+ 3.3 mM Macaca mulatta
2.5.1.18 Co2+ 3.3 mM Macaca mulatta
2.5.1.18 Cu2+ 3.3 mM Macaca mulatta
2.5.1.18 guanidine hydrochloride reversible denaturation at 3.2 M Macaca mulatta
2.5.1.18 Hg2+ 3.3 mM Macaca mulatta
2.5.1.18 iodoacetate weak Macaca mulatta
2.5.1.18 N-ethylmaleimide weak Macaca mulatta
2.5.1.18 Ni2+ 3.3 mM Macaca mulatta
2.5.1.18 Phenylglyoxal
-
 Macaca mulatta
2.5.1.18 Sodium dodecyl sulfate denaturates irreversibly at 0.03 M Macaca mulatta
2.5.1.18 Urea denaturates at 7.2 M, reversible Macaca mulatta
2.5.1.18 vinylpyridine weak Macaca mulatta
2.5.1.18 Zn2+ 3.3 mM Macaca mulatta

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.18 0.25
-
 glutathione
-
 Macaca mulatta
2.5.1.18 1.4
-
 1,2-dichloro-4-nitrobenzene
-
 Macaca mulatta

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.18 24000
-
 2 * 24000, SDS-PAGE Macaca mulatta
2.5.1.18 48000
-
 isoenzyme Macaca mulatta

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
2.5.1.18 Acetone stable for 2 h at 5% and 25% acetone Macaca mulatta
2.5.1.18 Butanol 90% inactivation at 25% butanol, stable for 2 h at 5% butanol Macaca mulatta
2.5.1.18 dimethyl sulfoxide 5% and 25% dimethyl sulfoxide: stable for 2 h Macaca mulatta
2.5.1.18 dimethylformamide stable for 2 h at 5% and 25% dimethylformamide Macaca mulatta
2.5.1.18 dioxane 5% dioxane: stable for 2 h Macaca mulatta
2.5.1.18 dioxane 25% dioxane: 25% loss of activity Macaca mulatta
2.5.1.18 Ethanol 5% and 25% ethanol: stable for 2 h Macaca mulatta
2.5.1.18 Methanol stable at 5% and 25% methanol for 2 h Macaca mulatta
2.5.1.18 methyl cellosolve stable at 5% and 25% methyl cellosolve for 2 h Macaca mulatta
2.5.1.18 propanol stable for 2 h at 5% propanol Macaca mulatta
2.5.1.18 propanol 90% loss of activity at 25% propanol Macaca mulatta

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.18 Macaca mulatta
-
 rhesus monkey
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.18
-
 Macaca mulatta

Renatured (Commentary)

EC Number Renatured (Comment) Organism
2.5.1.18 partial regeneration after urea and guanidine hydrochloride denaturation Macaca mulatta

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.5.1.18 liver
-
 Macaca mulatta
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.18 glutathione + 1,2-dichloro-4-nitrobenzene
-
 Macaca mulatta ?
-
 ?
2.5.1.18 glutathione + 4-nitrobenzyl chloride
-
 Macaca mulatta ?
-
 ?
2.5.1.18 glutathione + 4-nitrophenyl bromide
-
 Macaca mulatta ?
-
 ?
2.5.1.18 glutathione + trans-4-phenyl-3-buten-2-one
-
 Macaca mulatta ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.18 dimer 2 * 24000, SDS-PAGE Macaca mulatta

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.5.1.18 37
-
 pH 7.5, 3 h, stable Macaca mulatta
2.5.1.18 40
-
 10 min, pH 7.5, stable up to 40°C Macaca mulatta
2.5.1.18 40
-
 moderately stable below Macaca mulatta
2.5.1.18 50
-
 complete inactivation within 1 h Macaca mulatta

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.18 7.5 9 1,2-dichloro-4-nitrobenzene Macaca mulatta

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.5.1.18 4.2
-
 10 min at 37°C, complete loss of activity, at lower pH values somewhat more stable Macaca mulatta
2.5.1.18 7 10 10 min, 37°C, stable Macaca mulatta