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Literature summary extracted from

  • Clark, A.G.; Drake, B.
    Purification and properties of glutathione S-transferases from larvae of Wiseana cervinata (1984), Biochem. J., 217, 41-50.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.18 NaCl 0.2 M, 50% inhibition Wiseana cervinata
2.5.1.18 S-(2,4-dinitrophenyl)glutathione
-
 Wiseana cervinata

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.18 22800
-
 2 * 22800 or 2 * 24600, SDS-PAGE Wiseana cervinata
2.5.1.18 24600
-
 2 * 22800 or 2 * 24600, SDS-PAGE Wiseana cervinata
2.5.1.18 45000 50000
-
 Wiseana cervinata

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.18 Wiseana cervinata
-
 porina moth
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.18
-
 Wiseana cervinata

Reaction

EC Number Reaction Comment Organism Reaction ID
2.5.1.18 RX + glutathione = HX + R-S-glutathione random steady state mechanism Wiseana cervinata
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.5.1.18 larva
-
 Wiseana cervinata
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.18 108
-
-
 Wiseana cervinata

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.18 glutathione + 1,2-dichloro-4-nitrobenzene
-
 Wiseana cervinata ?
-
 ?
2.5.1.18 glutathione + 1-chloro-2,4-dinitrobenzene
-
 Wiseana cervinata S-2,4-dinitrophenylglutathione + HCl
-
 ?
2.5.1.18 glutathione + 4-nitrobenzyl chloride
-
 Wiseana cervinata ?
-
 ?
2.5.1.18 glutathione + ethacrynic acid
-
 Wiseana cervinata ?
-
 ?
2.5.1.18 glutathione + indomethane
-
 Wiseana cervinata ?
-
 ?
2.5.1.18 glutathione + iodomethane
-
 Wiseana cervinata ?
-
 ?
2.5.1.18 additional information specificity Wiseana cervinata ?
-
 ?
2.5.1.18 additional information no substrate: bromosulfophthalein Wiseana cervinata ?
-
 ?
2.5.1.18 additional information no substrate: 4-nitrobenzyl chloride Wiseana cervinata ?
-
 ?
2.5.1.18 additional information no substrate: 4-nitropyridine N-oxide Wiseana cervinata ?
-
 ?
2.5.1.18 additional information no substrate: 1,2-epoxy-3-(4-nitrophenoxy)propane Wiseana cervinata ?
-
 ?
2.5.1.18 RX + glutathione RX: R: aliphatic, aromatic or heterocyclic, X: sulfate, nitrite or halide, enzyme also catalyzes: the addition of aliphatic epoxides and arene oxides to glutathione, the reduction of polyol nitrate by glutathione to polyol and nitrite, certain isomerization reactions and disulfide interchange Wiseana cervinata HX + R-S-glutathione
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.18 dimer 2 * 22800 or 2 * 24600, SDS-PAGE Wiseana cervinata

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.18 7.5 8.5
-
 Wiseana cervinata