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Literature summary extracted from

  • Vander Jagt, D.L.; Hunsaker, L.A.; Garcia, K.B.; Royer, R.E.
    Isolation and characterization of the multiple glutathione S-transferases from human liver. Evidence for unique heme-binding sites (1985), J. Biol. Chem., 260, 11603-11610.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.18 bilirubin 50% inhibition at 0.01-0.02 mM Homo sapiens
2.5.1.18 Hematin non competitive with transferase activity Homo sapiens
2.5.1.18 N-ethylmaleimide completely inhibits form IX and X at 20 mM after 30 min, but not forms I-VIII Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.18 25200
-
 2 * 25200, form I-XII, SDS-PAGE Homo sapiens
2.5.1.18 25600
-
 2 * 25600, form XIII, SDS-PAGE Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.18 Homo sapiens
-
 13 forms
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.18 13 isoforms Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.5.1.18 erythrocyte
-
 Homo sapiens
-
2.5.1.18 liver
-
 Homo sapiens
-
2.5.1.18 placenta
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.18 1.5 8.3 with cumene hydroperoxide as substrate Homo sapiens
2.5.1.18 33 94 with 1-chloro-2,4-dinitrobenzene as substrate Homo sapiens
2.5.1.18 104
-
-
 Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.18 glutathione + 1-chloro-2,4-dinitrobenzene
-
 Homo sapiens S-2,4-dinitrophenylglutathione + HCl
-
 ?
2.5.1.18 glutathione + cumene hydroperoxide
-
 Homo sapiens ?
-
 ?
2.5.1.18 RX + glutathione RX: R: aliphatic, aromatic or heterocyclic, X: sulfate, nitrite or halide, enzyme also catalyzes: the addition of aliphatic epoxides and arene oxides to glutathione, the reduction of polyol nitrate by glutathione to polyol and nitrite, certain isomerization reactions and disulfide interchange Homo sapiens HX + R-S-glutathione
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.18 dimer 2 * 25600, form XIII, SDS-PAGE Homo sapiens
2.5.1.18 dimer 2 * 25200, form I-XII, SDS-PAGE Homo sapiens