Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Iwata-Reuyl, D.; Math, S.K.; Desai, S.B.; Poulter, C.D.
    Bacterial phytoene synthase: molecular cloning, expression, and characterization of Erwinia herbicola phytoene synthase (2003), Biochemistry, 42, 3359-3365.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.5.1.32 Tween 80 detergent required, maximal activity at 0.08% Pantoea agglomerans

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.32 expression in Escherichia coli Pantoea agglomerans

Protein Variants

EC Number Protein Variants Comment Organism
2.5.1.32 additional information to facilitate purification of the recombinant enzyme, the structural gene for Psase is modified by site-directed mutagenesis to incorporate a C-terminal Glu-Glu-Phe tripeptide to allow purification by immunoaffinity chromatography on an immobilized monoclonal anti-alpha-tubulin antibody YL1/2 column Pantoea agglomerans

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.5.1.32 soluble upon induction the recombinant enzyme constitutes 5-10% of the total soluble protein Pantoea agglomerans
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.32 Mn2+ required Pantoea agglomerans
2.5.1.32 Mn2+ maximal activity at 0.25 mM Pantoea agglomerans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.32 34500
-
 x * 34500, recombinant enzyme, SDS-PAGE Pantoea agglomerans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.32 geranylgeranyl diphosphate + geranylgeranyl diphosphate Pantoea agglomerans first pathway-specific step in carotenoid biosynthesis prephytoene diphosphate + diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.32 Pantoea agglomerans
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.32 to facilitate purification of the recombinant enzyme, the structural gene for Psase is modified by site-directed mutagenesis to incorporate a C-terminal Glu-Glu-Phe tripeptide to allow purification by immunoaffinity chromatography on an immobilized monoclonal anti-alpha-tubulin antibody YL1/2 column Pantoea agglomerans

Storage Stability

EC Number Storage Stability Organism
2.5.1.32 flash frozen in liquid N2 and stored at -80°C, stable for at least 6 months Pantoea agglomerans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.32 geranylgeranyl diphosphate + geranylgeranyl diphosphate the same enzyme catalyzes two consecutive reactions: 1. the synthesis of prephytoene diphosphate and 2. the synthesis of phytoene Pantoea agglomerans prephytoene diphosphate + diphosphate
-
 ?
2.5.1.32 geranylgeranyl diphosphate + geranylgeranyl diphosphate first pathway-specific step in carotenoid biosynthesis Pantoea agglomerans prephytoene diphosphate + diphosphate
-
 ?
2.5.1.32 prephytoene diphosphate the same enzyme catalyzes two consecutive reactions: 1. the synthesis of prephytoene diphosphate and 2. the synthesis of phytoene Pantoea agglomerans phytoene + diphosphate 15,15'-Z-phytoene is the sole product ?

Subunits

EC Number Subunits Comment Organism
2.5.1.32 ? x * 34500, recombinant enzyme, SDS-PAGE Pantoea agglomerans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.32 8.2
-
-
 Pantoea agglomerans

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.5.1.32 6.8 8.8 about 70% of maximal activity at pH 6.8 and pH 8.8 Pantoea agglomerans