Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Sasaki, T.; Tanaka, T.; Nakagawa, S.; Kainuma, K.
    Purification and properties of Cellvibrio gilvus cellobiose phosphorylase (1983), Biochem. J., 209, 803-807.
    View publication on PubMedView publication on EuropePMC

General Stability

EC Number General Stability Organism
2.4.1.20 storage of purified enzyme at -20°C without thiol reagents causes rapid loss of activity Cellulomonas gilvus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.20 alpha-oxogluconate
-
 Cellulomonas gilvus
2.4.1.20 glucono-delta-lactone very weak Cellulomonas gilvus
2.4.1.20 N-ethylmaleimide 56% inhibition at 0.5 mM Cellulomonas gilvus
2.4.1.20 Nojirimycin competitive with respect to cellobiose Cellulomonas gilvus
2.4.1.20 p-chloromercuribenzoate complete inhibition at 0.02 mM Cellulomonas gilvus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.20 0.77
-
 phosphate
-
 Cellulomonas gilvus
2.4.1.20 1.25
-
 cellobiose
-
 Cellulomonas gilvus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.20 Mg2+ required Cellulomonas gilvus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.20 72000
-
 SDS-PAGE Cellulomonas gilvus
2.4.1.20 280000
-
 gel filtration Cellulomonas gilvus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.20 cellobiose + phosphate Cellulomonas gilvus
-
 alpha-D-glucose 1-phosphate + D-glucose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.20 Cellulomonas gilvus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.20
-
 Cellulomonas gilvus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.20 27.4
-
 purified enzyme Cellulomonas gilvus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.20 cellobiose + phosphate
-
 Cellulomonas gilvus alpha-D-glucose 1-phosphate + D-glucose
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.20 tetramer 4 * 72000, gel filtration, SDS-PAGE Cellulomonas gilvus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.20 40
-
 inactivated above Cellulomonas gilvus
2.4.1.20 60
-
 complete inactivation within 10 min Cellulomonas gilvus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.20 7.6
-
-
 Cellulomonas gilvus